Anti-Liver Arginase antibody [RM1096]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [RM1096] (AB315110)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Liver Arginase with ab315110 at 1/5000 (0.093 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive cytoplasmic and nuclear staining on human liver. The section was incubated with ab315110 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [RM1096] (AB315110)

Immunohistochemical analysis of paraffin-embedded human brain tissue labeling liver arginase with ab315110 at 1/5000 (0.093 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human brain. The section was incubated with ab315110 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-Liver Arginase antibody [RM1096] (AB315110)

Liver Arginase was immunoprecipitated from 0.35 mg human liver tissue lysate with ab315110 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315110 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Human liver tissue lysate Lane 2 : ab315110 IP in Human liver tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab315110 in human liver whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Liver Arginase antibody [RM1096] (ab315110) at 1/30 dilution

All lanes:

Human liver tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [RM1096] (AB315110)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling liver arginase with ab315110 at 1/5000 (0.093 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive cytoplasmic and nuclear staining on rat liver. The section was incubated with ab315110 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [RM1096] (AB315110)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling liver arginase with ab315110 at 1/5000 (0.093 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse spleen. The section was incubated with ab315110 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [RM1096] (AB315110)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Liver Arginase with ab315110 at 1/5000 (0.093 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive cytoplasmic and nuclear staining on mouse liver. The section was incubated with ab315110 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [RM1096] (AB315110)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling liver arginase with ab315110 at 1/5000 (0.093 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on rat spleen. The section was incubated with ab315110 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-Liver Arginase antibody [RM1096] (AB315110)

Liver Arginase was immunoprecipitated from 0.35 mg mouse liver tissue lysate with ab315110 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315110 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Mouse liver tissue lysate Lane 2 : ab315110 IP in Mouse liver tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab315110 in mouse liver whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Liver Arginase antibody [RM1096] (ab315110) at 1/30 dilution

All lanes:

Mouse liver tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 32s

• IP

Supplier Data

Immunoprecipitation - Anti-Liver Arginase antibody [RM1096] (AB315110)

Liver Arginase was immunoprecipitated from 0.35 mg rat liver tissue lysate with ab315110 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315110 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Rat liver tissue lysate Lane 2 : ab315110 IP in Rat liver tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab315110 in rat liver whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Liver Arginase antibody [RM1096] (ab315110) at 1/30 dilution

All lanes:

Rat liver tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 32s

• WB

Supplier Data

Western blot - Anti-Liver Arginase antibody [RM1096] (AB315110)

Blocking and diluting buffer and concentration : Intercept® (TBS) Blocking Buffer diluted with an equal volume of TBS. Western blot : Anti-Liver Arginase antibody (ab315110) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5](ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab315110 was shown to bind specifically to Liver Arginase. Target of interest was observed at 35 kDa in wild-type HepG2 cell lysates (lane 1) with no signal observed at this size in ARG1 knockout cell line (lane 2, knockout cell line ab281603/ knockout cell lysate ab283099). To generate this image, samples were first run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in a fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed in TBS-T, incubated with secondary antibodies Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution for 1 h at room temperature, washed again then imaged.

All lanes:

Western blot - Anti-Liver Arginase antibody [RM1096] (ab315110) at 1/1000 dilution

Lane 1:

Wild-type HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Western blot - Human ARG1 knockout Hep G2 cell lysate (ab283099) at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (800CW) and Goat Anti-Mouse IgG H&L (680RD) at 1/20000 dilution

Observed band size: 35 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-Liver Arginase antibody [RM1096] (AB315110)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : spleen (PMID : 21975054). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Liver Arginase antibody [RM1096] (ab315110) at 1/1000 dilution

Lane 1:

Human liver tissue lysates at 20 µg

Lane 2:

Human spleen tissue lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 35 kDa,36 kDa

false

Exposure time: 6s

• WB

Supplier Data

Western blot - Anti-Liver Arginase antibody [RM1096] (AB315110)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : kidney and spleen (PMID : 21975054). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Liver Arginase antibody [RM1096] (ab315110) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysates at 20 µg

Lane 2:

Mouse kidney tissue lysates at 20 µg

Lane 3:

Mouse spleen tissue lysates at 20 µg

Lane 4:

Rat liver tissue lysates at 20 µg

Lane 5:

Rat kidney tissue lysates at 20 µg

Lane 6:

Rat spleen tissue lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 35 kDa,36 kDa

false

Exposure time: 15s