AB183333

Anti-Liver Arginase antibody [SP156]

RabMAb
Recombinant
What is this?

(1 Review)

(6 Publications)

Rabbit Recombinant Monoclonal Liver Arginase antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 6 publications.

View Alternative Names

Arginase-1, Liver-type arginase, Type I arginase, ARG1

13 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemical analysis of paraffin-embedded Human liver cirrhosis tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemical analysis of paraffin-embedded Human liver cirrhosis tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocyte cancer tissue sections labeling Liver Arginase with Purified ab183333 at 1/100 dilution (0.54 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

Lab

Western blot - Anti-Liver Arginase antibody [SP156] (AB183333)

Exposure time :
Lane1 & 2 is 3min
Lane 3 & 4 is 4 s

All lanes:

Western blot - Anti-Liver Arginase antibody [SP156] (ab183333) at 1.866 µg/mL

Lane 1:

Mouse liver tissue at 20 µg

Lane 2:

Mouse spleen tissue at 20 µg

Lane 3:

Human liver tissue at 20 µg

Lane 4:

Human spleen tissue at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL

Predicted band size: 35 kDa

Observed band size: 37 kDa

false

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Liver Arginase antibody [SP156]
Carrier free

Anti-Liver Arginase antibody [SP156] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP156

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1.86 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Boil tissue section in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1.86 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by protein A/G.

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed : 15546957, PubMed : 16709924, PubMed : 19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.

See full target information ARG1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 20:835 PubMed40999516

2025

Neuregulin 4 inhibits synovial macrophage pro-inflammatory polarization via ErbB4/Stat5b/NF-κB signaling to alleviate osteoarthritis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Daoliang Jiang,Xiaoli Xu,Wenjing Yang,Mengjia Tang,Yuping Zhang,Lingfeng Shi,Jiongyu Hu

American journal of cancer research 14:1139-1156 PubMed38590399

2024

PU.1 induces tumor-associated macrophages promoting glioma progression through BTK-mediated Akt/mTOR pathway activation.

Applications

Unspecified application

Species

Unspecified reactive species

Gu Song,Zeyu Zhang,Yan Chen,Weiliang Hou,Weiwei Zhong,Yuhang Zhou,Anke Zhang,Yuanzhi Xu

Journal of immunology research 2022:4903333 PubMed35879950

2022

Flavokawain B Weakens Gastric Cancer Progression via the TGF-1/SMAD4 Pathway and Attenuates M2 Macrophage Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Yongzhao Zhu,Weining Fan,Yuanzhen Wang,Huan Ding,Shaoqi Yang,Fang He

Clinical and experimental dermatology 47:675-683 PubMed34669971

2021

Interleukin-17E, inducible nitric oxide synthase and arginase1 as new biomarkers in the identification of neutrophilic dermatoses.

Applications

Unspecified application

Species

Unspecified reactive species

R Stalder,N Brembilla,C Conrad,N Yawalkar,A Navarini,W H Boehncke,G Kaya

Experimental dermatology 29:71-78 PubMed31721311

2019

The Janus Kinase inhibitor tofacitinib impacts human dendritic cell differentiation and favours M1 macrophage development.

Applications

Unspecified application

Species

Unspecified reactive species

Romaine Stalder,Bin Zhang,Ludovic Jean Wrobel,Wolf-Henning Boehncke,Nicolo Costantino Brembilla

Tissue engineering. Part C, Methods 23:754-762 PubMed28762881

2017

Porcine Ischemic Wound-Healing Model for Preclinical Testing of Degradable Biomaterials.

Applications

Unspecified application

Species

Unspecified reactive species

Prarthana Patil,John R Martin,Samantha M Sarett,Alonda C Pollins,Nancy L Cardwell,Jeffrey M Davidson,Scott A Guelcher,Lillian B Nanney,Craig L Duvall

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Liver Arginase antibody [SP156]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333)

Western blot - Anti-Liver Arginase antibody [SP156] (AB183333)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

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