Anti-Liver Arginase antibody [SP156] (ab183333)

Rabbit Monoclonal Liver Arginase antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 4 publications.

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (AB183333), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P
Human	Tested	Tested
Mouse	Tested	Expected
Cow	Predicted	Predicted
Pig	Predicted	Predicted
Rabbit	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1.86 µg/mL	Notes -
Species Human	Dilution info 1.86 µg/mL	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rabbit, Cow, Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes Boil tissue section in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rabbit, Cow, Pig	Dilution info -	Notes -

Associated Products

Select an associated product type

13 products for Alternative Product

Target data

See full target information ARG1

Function

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed:15546957, PubMed:16709924, PubMed:19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.

Alternative names

Arginase-1, Liver-type arginase, Type I arginase, ARG1

Recommended products

Rabbit Monoclonal Liver Arginase antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 4 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: SP156
Purification technique: Affinity purification Protein A/G
Concentration
Purification notes: Purified from TCS by protein A/G.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What are the advantages of a recombinant monoclonal antibody?

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocyte cancer tissue sections labeling Liver Arginase with Purified ab183333 at 1/100 dilution (0.54 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-Liver Arginase antibody [SP156] (ab183333)
Exposure time:
Lane1 & 2 is 3min
Lane 3 & 4 is 4 s
All lanes: Western blot - Anti-Liver Arginase antibody [SP156] (ab183333) at 1.866 µg/mL
Lane 1: Mouse liver tissue at 20 µg
Lane 2: Mouse spleen tissue at 20 µg
Lane 3: Human liver tissue at 20 µg
Lane 4: Human spleen tissue at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 0.05 µg/mL
Predicted band size: 35 kDa
Observed band size: 37 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)
Immunohistochemical analysis of paraffin-embedded Human liver cirrhosis tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)
Immunohistochemical analysis of paraffin-embedded Human liver cirrhosis tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [SP156] (ab183333)
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.