Rabbit Recombinant Monoclonal Liver Carboxylesterase 1/CES1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 5 publications.
Images
![Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (AB68190), expandable thumbnail](https://content.abcam.com/products/images/liver-carboxylesterase-1-ces1-antibody-ep1375y-ab68190--western-blot-img20571.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (AB68190)")
![Flow Cytometry (Intracellular) - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (AB68190), expandable thumbnail](https://content.abcam.com/products/images/liver-carboxylesterase-1-ces1-antibody-ep1375y-ab68190--flow-cytometry-intracellular-img29291.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (AB68190)")
Publications
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- Proteomics 22:e21000282022Effects of ABCG1 knockout on proteomic composition of HDL in mice on a chow diet and a high-fat diet.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanxiang Wu,Lianfeng Chen,Ziyan Xie,Chenyu Wang,Jiahao Zhang,Xiaowei YanPubMed 35234362
- Cells 11:2022Phosphorylation-Induced Ubiquitination and Degradation of PXR through CDK2-TRIM21 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengyao Qin,Yu Xin,Yong Bian,Xuan Yang,Tao Xi,Jing XiongPubMed 35053380
- British journal of pharmacology 179:46-642021Is platelet responsiveness to clopidogrel attenuated in overweight or obese patients and why? A reverse translational study in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Ping Jiang,Jin-Zi Ji,Peng-Xin Ge,Ting Zhu,Qiong-Yu Mi,Ting Tai,Yi-Fei Li,Hong-Guang XiePubMed 34415054
- Nature chemical biology 16:50-592019CPSF3-dependent pre-mRNA processing as a druggable node in AML and Ewing's sarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNathan T Ross,Felix Lohmann,Seth Carbonneau,Aleem Fazal,Wilhelm A Weihofen,Scott Gleim,Michael Salcius,Frederic Sigoillot,Martin Henault,Sarah H Carl,Juan B Rodríguez-Molina,Howard R Miller,Scott M Brittain,Jason Murphy,Mark Zambrowski,Geoffrey Boynton,Yuan Wang,Aye Chen,Gregory J Molind,Johannes H Wilbertz,Caroline G Artus-Revel,Min Jia,Favour A Akinjiyan,Jonathan Turner,Judith Knehr,Walter Carbone,Sven Schuierer,John S Reece-Hoyes,Kevin Xie,Chitra Saran,Eric T Williams,Guglielmo Roma,Matt Spencer,Jeremy Jenkins,Elizabeth L George,Jason R Thomas,Gregory Michaud,Markus Schirle,John Tallarico,Lori A Passmore,Jeffrey A Chao,Rohan E J BeckwithPubMed 31819276
- Toxicology reports 3:98-1042015Sodium sulfite promotes the assembly and secretion of very low-density lipoprotein in HL-7702 hepatocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianying Bai,Peiyu Lei,Chunyan Zhao,Youping Wang,Dandan Yan,Shuyun YangPubMed 28959527
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Tested | Not recommended | Not recommended | Expected |
| Rat | Tested | Not recommended | Not recommended | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/25000 - 1/50000 | Notes - |
Species Rat | Dilution info 1/25000 - 1/50000 | Notes - |
Species Human | Dilution info 1/25000 - 1/50000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/70 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs (PubMed:18762277, PubMed:7980644, PubMed:9169443, PubMed:9490062). Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester (PubMed:18762277, PubMed:7980644, PubMed:9169443, PubMed:9490062). Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine (PubMed:7980644). Catalyzes the transesterification of cocaine to form cocaethylene (PubMed:7980644). Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate (PubMed:7980644). Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes of 2-arachidonoylglycerol and prostaglandins (PubMed:21049984). Hydrolyzes cellular cholesteryl esters to free cholesterols and promotes reverse cholesterol transport (RCT) by facilitating both the initial and final steps in the process (PubMed:11015575, PubMed:16024911, PubMed:16971496, PubMed:18762277). First of all, allows free cholesterol efflux from macrophages to extracellular cholesterol acceptors and secondly, releases free cholesterol from lipoprotein-delivered cholesteryl esters in the liver for bile acid synthesis or direct secretion into the bile (PubMed:16971496, PubMed:18599737, PubMed:18762277).
Alternative names
CES2, SES1, CES1, Liver carboxylesterase 1, Acyl-coenzyme A:cholesterol acyltransferase, Brain carboxylesterase hBr1, Carboxylesterase 1, Cholesteryl ester hydrolase, Cocaine carboxylesterase, Egasyn, HMSE, Methylumbelliferyl-acetate deacetylase 1, Monocyte/macrophage serine esterase, Retinyl ester hydrolase, Serine esterase 1, Triacylglycerol hydrolase, ACAT, CE-1, hCE-1, CEH, REH, TGH
Recommended products
Rabbit Recombinant Monoclonal Liver Carboxylesterase 1/CES1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP1375Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (ab68190), expandable thumbnail](https://content.abcam.com/products/images/liver-carboxylesterase-1-ces1-antibody-ep1375y-ab68190--western-blot-img20571.jpg "Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (ab68190)")
Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (ab68190)
The molecular weight observed is consistent with what has been described in the literature (PMID: 21049984 and PMID: 30513753).
Blocking Buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (ab68190) at 1/50000 dilution
Lane 1: U-937 (Human histiocytic lymphoma monocyte) whole cell lysate at 15 µg
Lane 2: Mouse kidney lysate at 15 µg
Lane 3: Rat kidney lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 60 kDa
![Flow Cytometry (Intracellular) - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (ab68190), expandable thumbnail](https://content.abcam.com/products/images/liver-carboxylesterase-1-ces1-antibody-ep1375y-ab68190--flow-cytometry-intracellular-img29291.jpg "Flow Cytometry (Intracellular) - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (ab68190)")
Flow Cytometry (Intracellular) - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (ab68190)
Intracellular Flow Cytometry analysis of U-937 (Human histiocytic lymphoma monocyte) cells labeling Liver Carboxylesterase 1/CES1 with Purified ab68190 at 1/70 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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