AB68190

Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y]

Name: Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (ab68190) | Abcam
Brand: Abcam Limited
SKU: AB68190
Price: 545 USD
Availability: InStock
Rating: 2 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(5 Publications)

Rabbit Recombinant Monoclonal Liver Carboxylesterase 1/CES1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 5 publications.

View Alternative Names

CES2, SES1, CES1, Liver carboxylesterase 1, Acyl-coenzyme A:cholesterol acyltransferase, Brain carboxylesterase hBr1, Carboxylesterase 1, Cholesteryl ester hydrolase, Cocaine carboxylesterase, Egasyn, HMSE, Methylumbelliferyl-acetate deacetylase 1, Monocyte/macrophage serine esterase, Retinyl ester hydrolase, Serine esterase 1, Triacylglycerol hydrolase, ACAT, CE-1, hCE-1, CEH, REH, TGH

2 Images

Flow Cytometry (Intracellular) - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (AB68190)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (AB68190)

Intracellular Flow Cytometry analysis of U-937 (Human histiocytic lymphoma monocyte) cells labeling Liver Carboxylesterase 1/CES1 with Purified ab68190 at 1/70 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Unknown

Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (AB68190)

The molecular weight observed is consistent with what has been described in the literature (PMID : 21049984 and PMID : 30513753).

Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (ab68190) at 1/50000 dilution

Lane 1:

U-937 (Human histiocytic lymphoma monocyte) whole cell lysate at 15 µg

Lane 2:

Mouse kidney lysate at 15 µg

Lane 3:

Rat kidney lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 62 kDa

Observed band size: 60 kDa

false

Carrier free

Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1375Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/25000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/70", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/25000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/25000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs (PubMed : 18762277, PubMed : 7980644, PubMed : 9169443, PubMed : 9490062). Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester (PubMed : 18762277, PubMed : 7980644, PubMed : 9169443, PubMed : 9490062). Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine (PubMed : 7980644). Catalyzes the transesterification of cocaine to form cocaethylene (PubMed : 7980644). Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate (PubMed : 7980644). Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes of 2-arachidonoylglycerol and prostaglandins (PubMed : 21049984). Hydrolyzes cellular cholesteryl esters to free cholesterols and promotes reverse cholesterol transport (RCT) by facilitating both the initial and final steps in the process (PubMed : 11015575, PubMed : 16024911, PubMed : 16971496, PubMed : 18762277). First of all, allows free cholesterol efflux from macrophages to extracellular cholesterol acceptors and secondly, releases free cholesterol from lipoprotein-delivered cholesteryl esters in the liver for bile acid synthesis or direct secretion into the bile (PubMed : 16971496, PubMed : 18599737, PubMed : 18762277).

See full target information CES1

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Proteomics 22:e2100028 PubMed35234362

2022

Effects of ABCG1 knockout on proteomic composition of HDL in mice on a chow diet and a high-fat diet.

Applications

Unspecified application

Species

Unspecified reactive species

Yanxiang Wu,Lianfeng Chen,Ziyan Xie,Chenyu Wang,Jiahao Zhang,Xiaowei Yan

Cells 11: PubMed35053380

2022

Phosphorylation-Induced Ubiquitination and Degradation of PXR through CDK2-TRIM21 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyao Qin,Yu Xin,Yong Bian,Xuan Yang,Tao Xi,Jing Xiong

British journal of pharmacology 179:46-64 PubMed34415054

2021

Is platelet responsiveness to clopidogrel attenuated in overweight or obese patients and why? A reverse translational study in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Ping Jiang,Jin-Zi Ji,Peng-Xin Ge,Ting Zhu,Qiong-Yu Mi,Ting Tai,Yi-Fei Li,Hong-Guang Xie

Nature chemical biology 16:50-59 PubMed31819276

2019

CPSF3-dependent pre-mRNA processing as a druggable node in AML and Ewing's sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Nathan T Ross,Felix Lohmann,Seth Carbonneau,Aleem Fazal,Wilhelm A Weihofen,Scott Gleim,Michael Salcius,Frederic Sigoillot,Martin Henault,Sarah H Carl,Juan B Rodríguez-Molina,Howard R Miller,Scott M Brittain,Jason Murphy,Mark Zambrowski,Geoffrey Boynton,Yuan Wang,Aye Chen,Gregory J Molind,Johannes H Wilbertz,Caroline G Artus-Revel,Min Jia,Favour A Akinjiyan,Jonathan Turner,Judith Knehr,Walter Carbone,Sven Schuierer,John S Reece-Hoyes,Kevin Xie,Chitra Saran,Eric T Williams,Guglielmo Roma,Matt Spencer,Jeremy Jenkins,Elizabeth L George,Jason R Thomas,Gregory Michaud,Markus Schirle,John Tallarico,Lori A Passmore,Jeffrey A Chao,Rohan E J Beckwith

Toxicology reports 3:98-104 PubMed28959527

2015

Sodium sulfite promotes the assembly and secretion of very low-density lipoprotein in HL-7702 hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jianying Bai,Peiyu Lei,Chunyan Zhao,Youping Wang,Dandan Yan,Shuyun Yang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y]

Flow Cytometry (Intracellular) - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (AB68190)

Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (AB68190)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise