Rabbit Recombinant Monoclonal Liver Carboxylesterase 1/CES1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] - BSA and Azide free (AB188033), expandable thumbnail](https://content.abcam.com/products/images/liver-carboxylesterase-1-ces1-antibody-ep1375y-bsa-and-azide-free-ab188033--western-blot-img20571.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] - BSA and Azide free (AB188033)")
![Flow Cytometry (Intracellular) - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] - BSA and Azide free (AB188033), expandable thumbnail](https://content.abcam.com/products/images/liver-carboxylesterase-1-ces1-antibody-ep1375y-bsa-and-azide-free-ab188033--flow-cytometry-intracellular-img29291.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] - BSA and Azide free (AB188033)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Tested | Not recommended | Not recommended | Expected |
| Rat | Tested | Not recommended | Not recommended | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs (PubMed:18762277, PubMed:7980644, PubMed:9169443, PubMed:9490062). Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester (PubMed:18762277, PubMed:7980644, PubMed:9169443, PubMed:9490062). Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine (PubMed:7980644). Catalyzes the transesterification of cocaine to form cocaethylene (PubMed:7980644). Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate (PubMed:7980644). Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes of 2-arachidonoylglycerol and prostaglandins (PubMed:21049984). Hydrolyzes cellular cholesteryl esters to free cholesterols and promotes reverse cholesterol transport (RCT) by facilitating both the initial and final steps in the process (PubMed:11015575, PubMed:16024911, PubMed:16971496, PubMed:18762277). First of all, allows free cholesterol efflux from macrophages to extracellular cholesterol acceptors and secondly, releases free cholesterol from lipoprotein-delivered cholesteryl esters in the liver for bile acid synthesis or direct secretion into the bile (PubMed:16971496, PubMed:18599737, PubMed:18762277).
Alternative names
CES2, SES1, CES1, Liver carboxylesterase 1, Acyl-coenzyme A:cholesterol acyltransferase, Brain carboxylesterase hBr1, Carboxylesterase 1, Cholesteryl ester hydrolase, Cocaine carboxylesterase, Egasyn, HMSE, Methylumbelliferyl-acetate deacetylase 1, Monocyte/macrophage serine esterase, Retinyl ester hydrolase, Serine esterase 1, Triacylglycerol hydrolase, ACAT, CE-1, hCE-1, CEH, REH, TGH
Recommended products
Rabbit Recombinant Monoclonal Liver Carboxylesterase 1/CES1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP1375Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab188033 is the carrier-free version of Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] ab68190.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] - BSA and Azide free (ab188033), expandable thumbnail](https://content.abcam.com/products/images/liver-carboxylesterase-1-ces1-antibody-ep1375y-bsa-and-azide-free-ab188033--western-blot-img20571.jpg "Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] - BSA and Azide free (ab188033)")
Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] - BSA and Azide free (ab188033)
This data was developed using Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] ab68190, the same antibody clone in a different buffer formulation.
The molecular weight observed is consistent with what has been described in the literature (PMID: 21049984 and PMID: 30513753).
Blocking Buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] ab68190) at 1/50000 dilution
Lane 1: U-937 (Human histiocytic lymphoma monocyte) whole cell lysate at 15 µg
Lane 2: Mouse kidney lysate at 15 µg
Lane 3: Rat kidney lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 60 kDa
![Flow Cytometry (Intracellular) - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] - BSA and Azide free (ab188033), expandable thumbnail](https://content.abcam.com/products/images/liver-carboxylesterase-1-ces1-antibody-ep1375y-bsa-and-azide-free-ab188033--flow-cytometry-intracellular-img29291.jpg "Flow Cytometry (Intracellular) - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] - BSA and Azide free (ab188033)")
Flow Cytometry (Intracellular) - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] - BSA and Azide free (ab188033)
This data was developed using Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] ab68190, the same antibody clone in a different buffer formulation.Intracellular Flow Cytometry analysis of U-937 (Human histiocytic lymphoma monocyte) cells labeling Liver Carboxylesterase 1/CES1 with Purified ab188033 at 1/70 dilution (10μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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