AB53008

Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal Liver Carboxylesterase 1/CES1 antibody. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CES2, SES1, CES1, Liver carboxylesterase 1, Acyl-coenzyme A:cholesterol acyltransferase, Brain carboxylesterase hBr1, Carboxylesterase 1, Cholesteryl ester hydrolase, Cocaine carboxylesterase, Egasyn, HMSE, Methylumbelliferyl-acetate deacetylase 1, Monocyte/macrophage serine esterase, Retinyl ester hydrolase, Serine esterase 1, Triacylglycerol hydrolase, ACAT, CE-1, hCE-1, CEH, REH, TGH

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y] (AB53008)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y] (AB53008)

ab53008, at a 1/250 dilution, staining Human SOAT 1 in Liver using Immunohistochemistry, Paraffin embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y] (AB53008)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y] (AB53008)

Immunocytochemistry analysis of U-937 (human histiocytic lymphoma monocyte) labeling Liver Carboxylesterase 1/CES1 with purified ab53008 at 1/50 dilution (5 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.15 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control : PBS instead of the primary antibody.

Lab

Immunoprecipitation - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y] (AB53008)

Purified ab53008 at 1/100 dilution (2μg) immunoprecipitating Liver Carboxylesterase 1/CES1 in U-937 whole cell lysate.
Lane 1 (input) : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate 10μg
Lane 2 (+) : ab53008 + U-937 whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab53008 in U-937 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 60 kDa

All lanes:

Immunoprecipitation - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y] (ab53008)

Predicted band size: 62 kDa

false

Unknown

Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y] (AB53008)

All lanes:

Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y] (ab53008) at 1/10000 dilution

All lanes:

U937 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP labelled at 1/2000 dilution

Predicted band size: 62 kDa

Observed band size: 60 kDa

false

Carrier free

Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1376Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs (PubMed : 18762277, PubMed : 7980644, PubMed : 9169443, PubMed : 9490062). Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester (PubMed : 18762277, PubMed : 7980644, PubMed : 9169443, PubMed : 9490062). Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine (PubMed : 7980644). Catalyzes the transesterification of cocaine to form cocaethylene (PubMed : 7980644). Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate (PubMed : 7980644). Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes of 2-arachidonoylglycerol and prostaglandins (PubMed : 21049984). Hydrolyzes cellular cholesteryl esters to free cholesterols and promotes reverse cholesterol transport (RCT) by facilitating both the initial and final steps in the process (PubMed : 11015575, PubMed : 16024911, PubMed : 16971496, PubMed : 18762277). First of all, allows free cholesterol efflux from macrophages to extracellular cholesterol acceptors and secondly, releases free cholesterol from lipoprotein-delivered cholesteryl esters in the liver for bile acid synthesis or direct secretion into the bile (PubMed : 16971496, PubMed : 18599737, PubMed : 18762277).

See full target information CES1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:785 PubMed39472448

2024

Nicotine-induced CHRNA5 activation modulates CES1 expression, impacting head and neck squamous cell carcinoma recurrence and metastasis via MEK/ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Feng,Wei Mao,Chenyang Yuan,Pin Dong,Yuying Liu

Acta pharmaceutica Sinica. B 13:618-631 PubMed36873183

2023

Carboxylesterase 1 family knockout alters drug disposition and lipid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Changpei Gan,Jing Wang,Alejandra Martínez-Chávez,Michel Hillebrand,Niels de Vries,Joke Beukers,Els Wagenaar,Yaogeng Wang,Maria C Lebre,Hilde Rosing,Sjoerd Klarenbeek,Rahmen Bin Ali,Colin Pritchard,Ivo Huijbers,Jos H Beijnen,Alfred H Schinkel

Cell systems 5:564-577.e12 PubMed29128334

2017

A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-κB Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ramy H Malty,Hiroyuki Aoki,Ashwani Kumar,Sadhna Phanse,Shahreen Amin,Qingzhou Zhang,Zoran Minic,Florian Goebels,Gabriel Musso,Zhuoran Wu,Hosam Abou-Tok,Michael Meyer,Viktor Deineko,Sandy Kassir,Vishaldeep Sidhu,Matthew Jessulat,Nichollas E Scott,Xuejian Xiong,James Vlasblom,Bhanu Prasad,Leonard J Foster,Tiziana Alberio,Barbara Garavaglia,Haiyuan Yu,Gary D Bader,Ken Nakamura,John Parkinson,Mohan Babu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y] (AB53008)

Immunocytochemistry/ Immunofluorescence - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y] (AB53008)

Immunoprecipitation - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y] (AB53008)

Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y] (AB53008)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (3)

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