Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y] - BSA and Azide free (AB235996)

ab53008, at a 1/250 dilution, staining Human SOAT 1 in Liver using Immunohistochemistry, Paraffin embedded tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53008).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y] - BSA and Azide free (AB235996)

This data was developed using the same antibody clone in a different buffer formulation (ab53008).
Immunocytochemistry analysis of U-937 (human histiocytic lymphoma monocyte) labeling Liver Carboxylesterase 1/CES1 with purified ab53008 at 1/50 dilution (5 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.15 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control : PBS instead of the primary antibody.

• IP

Lab

Immunoprecipitation - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y] - BSA and Azide free (AB235996)

This data was developed using ab53008, the same antibody clone in a different buffer formulation.
Purified ab53008 at 1/100 dilution (2μg) immunoprecipitating Liver Carboxylesterase 1/CES1 in U-937 whole cell lysate.
Lane 1 (input) : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate 10μg
Lane 2 (+) : ab53008 + U-937 whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab53008 in U-937 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 60 kDa

All lanes:

Immunoprecipitation - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y] (<a href='/en-us/products/primary-antibodies/liver-carboxylesterase-1-ces1-antibody-ep1376y-ab53008'>ab53008</a>)

Predicted band size: 62 kDa

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