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Anti-liver FABP antibody [2G4] (ab82157) is a mouse monoclonal antibody detecting liver FABP in Western Blot, Flow Cytometry, ICC/IF. Suitable for Human,.



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Images

Western blot - Anti-liver FABP antibody [2G4] (AB82157), expandable thumbnail
  • Flow Cytometry - Anti-liver FABP antibody [2G4] (AB82157), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-liver FABP antibody [2G4] (AB82157), expandable thumbnail
  • Flow Cytometry - Anti-liver FABP antibody [2G4] (AB82157), expandable thumbnail
  • Western blot - Anti-liver FABP antibody [2G4] (AB82157), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 10% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human FABP1. Database link P07148

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IF
Human
Tested
Tested
Tested
Recombinant full length protein - Human
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Human, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PubMed:25732850). Binds cholesterol (PubMed:25732850). Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport (By similarity).

Alternative names

FABPL, FABP1, Fatty acid-binding protein 1, Liver-type fatty acid-binding protein, L-FABP

Recommended products

Anti-liver FABP antibody [2G4] (ab82157) is a mouse monoclonal antibody detecting liver FABP in Western Blot, Flow Cytometry, ICC/IF. Suitable for Human,.



- Trusted since 2009

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human FABP1. Database link P07148
Clone number
2G4
Purification technique
Affinity purification Protein A
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Notes

What is this antibody validated in?


Anti-liver FABP antibody [2G4] (ab82157) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, samples.

What is the molecular weight of liver FABP?


Anti-liver FABP [2G4] (ab82157) specifically detects a band for liver FABP (UniProt: P07148) at a molecular weight of 14kDa.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Liver fatty acid binding protein (liver FABP) also known as L-FABP or FABP1 is a small protein with a mass of approximately 14 kilodaltons. It functions mainly in the liver where it binds free fatty acids and other lipophilic substances facilitating their transport within cells. This protein is highly expressed in hepatocytes and also found in the small intestine and kidneys. Its role in binding fatty acids positions it as an important mediator in lipid metabolism making it of interest in a variety of metabolic studies.

Biological function summary

Liver FABP is essential for maintaining lipid homeostasis within cells. It is not part of a larger complex but acts by itself to regulate the intracellular concentration of lipids and protect cells from lipotoxicity. By sequestering fatty acids it prevents these molecules from disrupting cellular membranes or signaling pathways. Liver FABP also participates in the uptake transport and metabolic conversion of fatty acids and their metabolites influencing energy homeostasis and other vital processes.

Pathways

Liver FABP plays a significant role in the fatty acid metabolism and peroxisome proliferator-activated receptor (PPAR) signaling pathways. It acts by modulating the availability of lipid ligands necessary for PPAR activation linking it functionally to these nuclear receptors that control gene expression involved in lipid and glucose metabolism. Liver FABP's association with proteins like FABP2 and FABP4 within these pathways provides insight into its broader metabolic network highlighting its interactions in fatty acid transport and metabolism.

Associated diseases and disorders

Liver FABP shows a strong connection to metabolic conditions particularly non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes. Its dysregulation is often observed in these disorders which include altered lipid profiles and insulin resistance. Liver FABP is also linked to certain forms of cancer with aberrant expression levels found in some tumor types. The interactions of liver FABP with proteins such as FABP5 in these diseases suggest a potential role in the pathogenesis of metabolic disorders and tumor development making it a candidate for further research and therapeutic targeting.

Product promise

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6 product images

  • Western blot - Anti-liver FABP antibody [2G4] (ab82157), expandable thumbnail

    Western blot - Anti-liver FABP antibody [2G4] (ab82157)

    All lanes: Western blot - Anti-liver FABP antibody [2G4] (ab82157) at 1/1000 dilution

    Lane 1: FABP1 Recombinant protein

    Lane 2: HepG2 cell lysate at 40 µg

    Lane 3: Liver tissues lysate at 40 µg

    Secondary

    All lanes: goat anti-mouse secondary antibody conjugated to HRP

    Predicted band size: 14 kDa

  • Flow Cytometry - Anti-liver FABP antibody [2G4] (ab82157), expandable thumbnail

    Flow Cytometry - Anti-liver FABP antibody [2G4] (ab82157)

    Flow cytometry analysis of FABP1 in Hep3B cells. The cell was stained with ab82157 at 2-5μg for 1x106 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).

  • Immunocytochemistry/ Immunofluorescence - Anti-liver FABP antibody [2G4] (ab82157), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-liver FABP antibody [2G4] (ab82157)

    Immunocytochemistry/ Immunofluorescence analysis of FABP1 in Hep3B cells. The cell was stained with ab82157 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

  • Flow Cytometry - Anti-liver FABP antibody [2G4] (ab82157), expandable thumbnail

    Flow Cytometry - Anti-liver FABP antibody [2G4] (ab82157)

    Overlay histogram showing HepG2 cells stained with ab82157 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82157, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This image was produced using the ascites version of this antibody.

  • Western blot - Anti-liver FABP antibody [2G4] (ab82157), expandable thumbnail

    Western blot - Anti-liver FABP antibody [2G4] (ab82157)

    This image was produced using the ascites version of this antibody.

    All lanes: Western blot - Anti-liver FABP antibody [2G4] (ab82157) at 1/1000 dilution

    All lanes: HepG2 cell lysate at 30 µg

    Secondary

    All lanes: goat anti-mouse HRP

    Developed using the ECL technique.

    Predicted band size: 14 kDa

    Observed band size: 14 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-liver FABP antibody [2G4] (ab82157), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-liver FABP antibody [2G4] (ab82157)

    ab82157 staining liver FABP in Hep3B cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Samples were incubated with primary antibody at 1:100 dilution. An Alexa Fluor® 488 goat anti-mouse IgG was used as the secondary antibody. DAPI was used as a nuclear counterstain.

    This image was produced using the ascites version of this antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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