Anti-liver FABP antibody [2G4]

• WB

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Western blot - Anti-liver FABP antibody [2G4] (AB82157)

All lanes:

Western blot - Anti-liver FABP antibody [2G4] (ab82157) at 1/1000 dilution

Lane 1:

FABP1 Recombinant protein

Lane 2:

HepG2 cell lysate at 40 µg

Lane 3:

Liver tissues lysate at 40 µg

Secondary

All lanes:

goat anti-mouse secondary antibody conjugated to HRP

Predicted band size: 14 kDa

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• Flow Cyt

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Flow Cytometry - Anti-liver FABP antibody [2G4] (AB82157)

Flow cytometry analysis of FABP1 in Hep3B cells. The cell was stained with ab82157 at 2-5μg for 1x10⁶ cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-liver FABP antibody [2G4] (AB82157)

ab82157 staining liver FABP in Hep3B cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Samples were incubated with primary antibody at 1 : 100 dilution. An Alexa Fluor^® 488 goat anti-mouse IgG was used as the secondary antibody. DAPI was used as a nuclear counterstain.

This image was produced using the ascites version of this antibody.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-liver FABP antibody [2G4] (AB82157)

Immunocytochemistry/ Immunofluorescence analysis of FABP1 in Hep3B cells. The cell was stained with ab82157 (1 : 100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

• Flow Cyt

Unknown

Flow Cytometry - Anti-liver FABP antibody [2G4] (AB82157)

Overlay histogram showing HepG2 cells stained with ab82157 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82157, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

This image was produced using the ascites version of this antibody.

• WB

Unknown

Western blot - Anti-liver FABP antibody [2G4] (AB82157)

This image was produced using the ascites version of this antibody.

All lanes:

Western blot - Anti-liver FABP antibody [2G4] (ab82157) at 1/1000 dilution

All lanes:

HepG2 cell lysate at 30 µg

Secondary

All lanes:

goat anti-mouse HRP

Predicted band size: 14 kDa

Observed band size: 14 kDa

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