Anti-liver FABP antibody [EPR20464]

• mIHC

Lab

Multiplex immunohistochemistry - Anti-liver FABP antibody [EPR20464] (AB222517)

Fluorescence multiplex immunohistochemical analysis of the human colon (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-liver FABP (ab240401 gray; Opal™690), anti-CD3 epsilon (ab16669 green; Opal™520) and anti-CD68 (ab213363 red; Opal™570) on human colon. Panel B : anti-liver FABP stained on enterocytes. Panel C : anti-CD3 epsilon stained on T cells. Panel D : anti-CD68 stained on macrophages. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining : in the order of ab240401 (1/8000 dilution), ab16669 (1/150 dilution) and ab213363 (1/500 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [EPR20464] (AB222517)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling liver FABP with ab222517 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and nuclear staining on human colon cancer (PMID : 15138477). Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [EPR20464] (AB222517)

Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling liver FABP with ab222517 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear and cytoplasmic staining on human gastric cancer (PMID : 15051923). Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-liver FABP antibody [EPR20464] (AB222517)

Fluorescence multiplex immunohistochemical analysis of the human colon (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-Villin (ab245749, gray; Opal™690), anti-liver FABP (ab240401, green; Opal™520) and anti-MUC2 (ab272692, red; Opal™570) on human colon. Panel B : anti-liver FABP stained on enterocytes. Panel C : anti-Villin stained on apical border. Panel D : anti-MUC2 stained on goblet cells. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining : in the order of ab245749 (1/1000 dilution), ab240401 (1/8000 dilution), and ab272692 (1/5000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-liver FABP antibody [EPR20464] (AB222517)

Fluorescence multiplex immunohistochemical analysis of the human duodenum (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-liver FABP (ab240401 gray; Opal™690), anti-CD3 epsilon (ab16669 green; Opal™520) and anti-CD68 (ab213363 red; Opal™570) on human duodenum. Panel B : anti-liver FABP stained on enterocytes. Panel C : anti-CD3 epsilon stained on T cells. Panel D : anti-CD68 stained on macrophages. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining : in the order of ab240401 (1/8000 dilution), ab16669 (1/150 dilution) and ab213363 (1/500 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [EPR20464] (AB222517)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling liver FABP with ab222517 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and weak nuclear staining on human hepatocellular carcinoma. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-liver FABP antibody [EPR20464] (AB222517)

Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% tritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling liver FABP with ab222517 at 1/100 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on HepG2 cell line. Nuclear counterstain DAPI (blue). Counterstain Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 dilution (red).

The negative control was secondary antibody only.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [EPR20464] (AB222517)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling liver FABP with ab222517 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on human hepatocytes and sinusoids (PMID : 3123629). Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [EPR20464] (AB222517)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling liver FABP with ab222517 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and weak nuclear staining on human colon (PMID : 15138477). Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-liver FABP antibody [EPR20464] (AB222517)

Fluorescence multiplex immunohistochemical analysis of the human duodenum (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-liver FABP (ab240401, gray; Opal™690), anti-Lysozyme (ab242430, green; Opal™520) and anti-Chromogranin A (ab254557, red; Opal™570) on human duodenum. Panel B : anti-liver FABP stained on enterocytes. Panel C : anti-Lysozyme stained on Paneth cells. Panel D : anti-Chromogranin A stained on neuroendocrine cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining : in the order of ab240401 (1/8000 dilution), ab242430 (1 : 250 dilution), and ab254557 (1/5000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [EPR20464] (AB222517)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling liver FABP with ab222517 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear and cytoplasmic staining on rat liver is observed. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

• WB

Lab

Western blot - Anti-liver FABP antibody [EPR20464] (AB222517)

Blocking buffer : 5% NFDM/TBST.

Exposure time :

Lanes 1-2 : 1 minute

Lane 3 : 30 seconds

Lane 4 : 5 seconds

Lane 5 : 1 second

The molecular weight observed is consistent with the literature (PMID : 21504868).

Lanes 1 - 4:

Western blot - Anti-liver FABP antibody [EPR20464] (ab222517) at 1/1000 dilution

Lane 5:

Western blot - Anti-liver FABP antibody [EPR20464] (ab222517) at 1/2000 dilution

Lane 1:

Human fetal colon lysate at 20 µg

Lane 2:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

Human fetal liver lysate at 20 µg

Lane 4:

Rat liver lysate at 20 µg

Lane 5:

Mouse liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 14 kDa

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