Anti-liver FABP antibody [EPR20464] - BSA and Azide free

11 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [EPR20464] - BSA and Azide free (ab240401)

  Immunohistochemical analysis of paraffin-embedded human liver tissue labeling liver FABP with Anti-liver FABP antibody [EPR20464] ab222517 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on human hepatocytes and sinusoids (PMID: 3123629). Counter stained with Hematoxylin.

  Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-liver FABP antibody [EPR20464] ab222517).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [EPR20464] - BSA and Azide free (ab240401)

  Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling liver FABP with Anti-liver FABP antibody [EPR20464] ab222517 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and weak nuclear staining on human hepatocellular carcinoma. Counter stained with Hematoxylin.

  Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-liver FABP antibody [EPR20464] ab222517).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [EPR20464] - BSA and Azide free (ab240401)

  Immunohistochemical analysis of paraffin-embedded human colon tissue labeling liver FABP with Anti-liver FABP antibody [EPR20464] ab222517 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and weak nuclear staining on human colon (PMID: 15138477). Counter stained with Hematoxylin.

  Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-liver FABP antibody [EPR20464] ab222517).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [EPR20464] - BSA and Azide free (ab240401)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling liver FABP with Anti-liver FABP antibody [EPR20464] ab222517 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and nuclear staining on human colon cancer (PMID: 15138477). Counter stained with Hematoxylin.

  Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-liver FABP antibody [EPR20464] ab222517).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [EPR20464] - BSA and Azide free (ab240401)

  Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling liver FABP with Anti-liver FABP antibody [EPR20464] ab222517 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear and cytoplasmic staining on human gastric cancer (PMID: 15051923). Counter stained with Hematoxylin.

  Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-liver FABP antibody [EPR20464] ab222517).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [EPR20464] - BSA and Azide free (ab240401)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling liver FABP with Anti-liver FABP antibody [EPR20464] ab222517 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear and cytoplasmic staining on rat liver is observed. Counter stained with Hematoxylin.

  Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-liver FABP antibody [EPR20464] ab222517).

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  Immunocytochemistry/ Immunofluorescence - Anti-liver FABP antibody [EPR20464] - BSA and Azide free (ab240401)

  Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% tritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling liver FABP with Anti-liver FABP antibody [EPR20464] ab222517 at 1/100 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on HepG2 cell line. Nuclear counterstain DAPI (blue). Counterstain Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 dilution (red).

  The negative control was secondary antibody only.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-liver FABP antibody [EPR20464] ab222517).

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  Multiplex immunohistochemistry - Anti-liver FABP antibody [EPR20464] - BSA and Azide free (ab240401)

  Fluorescence multiplex immunohistochemical analysis of the human colon (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-Villin (Anti-Villin antibody [SP145] - BSA and Azide free ab245749, gray; Opal™690), anti-liver FABP (ab240401, green; Opal™520) and anti-MUC2 (Anti-MUC2 antibody [EPR23479-47] ab272692, red; Opal™570) on human colon. Panel B: anti-liver FABP stained on enterocytes. Panel C: anti-Villin stained on apical border. Panel D: anti-MUC2 stained on goblet cells. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-Villin antibody [SP145] - BSA and Azide free ab245749 (1/1000 dilution), ab240401 (1/8000 dilution), and Anti-MUC2 antibody [EPR23479-47] ab272692 (1/5000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
  
  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-liver FABP antibody [EPR20464] ab222517).

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  Multiplex immunohistochemistry - Anti-liver FABP antibody [EPR20464] - BSA and Azide free (ab240401)

  Liver FABP immunohistochemistry staining of human duodenum using rabbit anti-liver FABP antibody
  
  

  Fluorescence multiplex immunohistochemical analysis of the human duodenum (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-liver FABP (ab240401 gray; Opal™690), anti-CD3 epsilon (Anti-CD3 epsilon antibody [SP7] ab16669 green; Opal™520) and anti-CD68 (Anti-CD68 antibody [EPR20545] ab213363 red; Opal™570) on human duodenum. Panel B: anti-liver FABP stained on enterocytes. Panel C: anti-CD3 epsilon stained on T cells. Panel D: anti-CD68 stained on macrophages. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of ab240401 (1/8000 dilution), Anti-CD3 epsilon antibody [SP7] ab16669 (1/150 dilution) and Anti-CD68 antibody [EPR20545] ab213363 (1/500 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-liver FABP antibody [EPR20464] ab222517).

• 

  Multiplex immunohistochemistry - Anti-liver FABP antibody [EPR20464] - BSA and Azide free (ab240401)

  Fluorescence multiplex immunohistochemical analysis of the human duodenum (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-liver FABP (ab240401, gray; Opal™690), anti-Lysozyme (Anti-Lysozyme antibody [SP350] - BSA and Azide free ab242430, green; Opal™520) and anti-Chromogranin A (Anti-Chromogranin A antibody [EPR22537-249] ab254557, red; Opal™570) on human duodenum. Panel B: anti-liver FABP stained on enterocytes. Panel C: anti-Lysozyme stained on Paneth cells. Panel D: anti-Chromogranin A stained on neuroendocrine cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of ab240401 (1/8000 dilution), Anti-Lysozyme antibody [SP350] - BSA and Azide free ab242430 (1:250 dilution), and Anti-Chromogranin A antibody [EPR22537-249] ab254557 (1/5000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-liver FABP antibody [EPR20464] ab222517).

• 

  Multiplex immunohistochemistry - Anti-liver FABP antibody [EPR20464] - BSA and Azide free (ab240401)

  Liver FABP immunohistochemistry staining of human colon using rabbit anti-liver FABP antibody
  
  

  Fluorescence multiplex immunohistochemical analysis of the human colon (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-liver FABP (ab240401 gray; Opal™690), anti-CD3 epsilon (Anti-CD3 epsilon antibody [SP7] ab16669 green; Opal™520) and anti-CD68 (Anti-CD68 antibody [EPR20545] ab213363 red; Opal™570) on human colon. Panel B: anti-liver FABP stained on enterocytes. Panel C: anti-CD3 epsilon stained on T cells. Panel D: anti-CD68 stained on macrophages. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of ab240401 (1/8000 dilution), Anti-CD3 epsilon antibody [SP7] ab16669 (1/150 dilution) and Anti-CD68 antibody [EPR20545] ab213363 (1/500 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-liver FABP antibody [EPR20464] ab222517).