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AB129203

Anti-liver FABP antibody [EPR5895(2)]

5

(1 Review)

|

(3 Publications)

Rabbit Recombinant Monoclonal liver FABP antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 3 publications.

View Alternative Names

FABPL, FABP1, Fatty acid-binding protein 1, Liver-type fatty acid-binding protein, L-FABP

3 Images
Flow Cytometry (Intracellular) - Anti-liver FABP antibody [EPR5895(2)] (AB129203)
  • Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-liver FABP antibody [EPR5895(2)] (AB129203)

ab129203, at 1/10 dilution, staining FABP in permeabilized HepG2 cells by intracellular flow cytometry (Red). Green represents rabbit IgG negative control.

Western blot - Anti-liver FABP antibody [EPR5895(2)] (AB129203)
  • WB

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Western blot - Anti-liver FABP antibody [EPR5895(2)] (AB129203)

All lanes:

Western blot - Anti-liver FABP antibody [EPR5895(2)] (ab129203) at 1/1000 dilution

Lane 1:

Fetal liver tissue lysate at 10 µg

Lane 2:

Mouse liver tissue lysate at 10 µg

Lane 3:

Human small intestine lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 14 kDa

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OI-RD Scanning - Anti-liver FABP antibody [EPR5895(2)] (AB129203)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-liver FABP antibody [EPR5895(2)] (AB129203)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-liver FABP antibody [EPR5895(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5895(2)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Liver fatty acid binding protein (liver FABP) also known as L-FABP or FABP1 is a small protein with a mass of approximately 14 kilodaltons. It functions mainly in the liver where it binds free fatty acids and other lipophilic substances facilitating their transport within cells. This protein is highly expressed in hepatocytes and also found in the small intestine and kidneys. Its role in binding fatty acids positions it as an important mediator in lipid metabolism making it of interest in a variety of metabolic studies.
Biological function summary

Liver FABP is essential for maintaining lipid homeostasis within cells. It is not part of a larger complex but acts by itself to regulate the intracellular concentration of lipids and protect cells from lipotoxicity. By sequestering fatty acids it prevents these molecules from disrupting cellular membranes or signaling pathways. Liver FABP also participates in the uptake transport and metabolic conversion of fatty acids and their metabolites influencing energy homeostasis and other vital processes.

Pathways

Liver FABP plays a significant role in the fatty acid metabolism and peroxisome proliferator-activated receptor (PPAR) signaling pathways. It acts by modulating the availability of lipid ligands necessary for PPAR activation linking it functionally to these nuclear receptors that control gene expression involved in lipid and glucose metabolism. Liver FABP's association with proteins like FABP2 and FABP4 within these pathways provides insight into its broader metabolic network highlighting its interactions in fatty acid transport and metabolism.

Liver FABP shows a strong connection to metabolic conditions particularly non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes. Its dysregulation is often observed in these disorders which include altered lipid profiles and insulin resistance. Liver FABP is also linked to certain forms of cancer with aberrant expression levels found in some tumor types. The interactions of liver FABP with proteins such as FABP5 in these diseases suggest a potential role in the pathogenesis of metabolic disorders and tumor development making it a candidate for further research and therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PubMed : 25732850). Binds cholesterol (PubMed : 25732850). Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport (By similarity).
See full target information FABP1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Clinical proteomics 15:34 PubMed30386187

2018

Proteomic profiling of liver tissue from the - mouse model of Duchenne muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Murphy,Margit Zweyer,Michael Henry,Paula Meleady,Rustam R Mundegar,Dieter Swandulla,Kay Ohlendieck

Nature 531:53-8 PubMed26935695

2016

High-fat diet enhances stemness and tumorigenicity of intestinal progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Semir Beyaz,Miyeko D Mana,Jatin Roper,Dmitriy Kedrin,Assieh Saadatpour,Sue-Jean Hong,Khristian E Bauer-Rowe,Michael E Xifaras,Adam Akkad,Erika Arias,Luca Pinello,Yarden Katz,Shweta Shinagare,Monther Abu-Remaileh,Maria M Mihaylova,Dudley W Lamming,Rizkullah Dogum,Guoji Guo,George W Bell,Martin Selig,G Petur Nielsen,Nitin Gupta,Cristina R Ferrone,Vikram Deshpande,Guo-Cheng Yuan,Stuart H Orkin,David M Sabatini,Ömer H Yilmaz

Experimental and therapeutic medicine 10:1777-1783 PubMed26640549

2015

Liraglutide reduces fatty degeneration in hepatic cells via the AMPK/SREBP1 pathway.

Applications

WB

Species

Human

Yan-Gui Wang,Tian-Lun Yang
View all publications
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