Mouse Monoclonal liver FABP antibody. Carrier free. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FABP1 aa 1 to C-terminus.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [FABP1/3482] - BSA and Azide free (AB268176), expandable thumbnail](https://content.abcam.com/products/images/liver-fabp-antibody-fabp1-3482-bsa-and-azide-free-ab268176--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65436.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [FABP1/3482] - BSA and Azide free (AB268176)")
![Western blot - Anti-liver FABP antibody [FABP1/3482] - BSA and Azide free (AB268176), expandable thumbnail](https://content.abcam.com/products/images/liver-fabp-antibody-fabp1-3482-bsa-and-azide-free-ab268176--western-blot-img29034.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-liver FABP antibody [FABP1/3482] - BSA and Azide free (AB268176)")
![Protein Array - Anti-liver FABP antibody [FABP1/3482] - BSA and Azide free (AB268176), expandable thumbnail](https://content.abcam.com/products/images/liver-fabp-antibody-fabp1-3482-bsa-and-azide-free-ab268176--protein-array-img140007.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-liver FABP antibody [FABP1/3482] - BSA and Azide free (AB268176)")
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Human FABP1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P07148
Reactivity data
Select an application| WB | Protein Array | IHC-P | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
6 products for Alternative Product
Target data
Function
Plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PubMed:25732850). Binds cholesterol (PubMed:25732850). Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport (By similarity).
Alternative names
Fatty acid-binding protein 1, Liver-type fatty acid-binding protein, L-FABP, FABP1, FABPL
Recommended products
Mouse Monoclonal liver FABP antibody. Carrier free. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FABP1 aa 1 to C-terminus.
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human FABP1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P07148
- Carrier free
Yes
- Clone number
FABP1/3482
- Purification technique
Affinity purification Protein A/G
- Light chain type
kappa
- Concentration
- Purification notes
Purified from Bioreactor concentrate
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
ab268176 is the carrier-free version of Anti-liver FABP antibody [FABP1/3482] ab268055.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Liver fatty acid binding protein (liver FABP) also known as L-FABP or FABP1 is a small protein with a mass of approximately 14 kilodaltons. It functions mainly in the liver where it binds free fatty acids and other lipophilic substances facilitating their transport within cells. This protein is highly expressed in hepatocytes and also found in the small intestine and kidneys. Its role in binding fatty acids positions it as an important mediator in lipid metabolism making it of interest in a variety of metabolic studies.
- Biological function summary
Liver FABP is essential for maintaining lipid homeostasis within cells. It is not part of a larger complex but acts by itself to regulate the intracellular concentration of lipids and protect cells from lipotoxicity. By sequestering fatty acids it prevents these molecules from disrupting cellular membranes or signaling pathways. Liver FABP also participates in the uptake transport and metabolic conversion of fatty acids and their metabolites influencing energy homeostasis and other vital processes.
- Pathways
Liver FABP plays a significant role in the fatty acid metabolism and peroxisome proliferator-activated receptor (PPAR) signaling pathways. It acts by modulating the availability of lipid ligands necessary for PPAR activation linking it functionally to these nuclear receptors that control gene expression involved in lipid and glucose metabolism. Liver FABP's association with proteins like FABP2 and FABP4 within these pathways provides insight into its broader metabolic network highlighting its interactions in fatty acid transport and metabolism.
- Associated diseases and disorders
Liver FABP shows a strong connection to metabolic conditions particularly non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes. Its dysregulation is often observed in these disorders which include altered lipid profiles and insulin resistance. Liver FABP is also linked to certain forms of cancer with aberrant expression levels found in some tumor types. The interactions of liver FABP with proteins such as FABP5 in these diseases suggest a potential role in the pathogenesis of metabolic disorders and tumor development making it a candidate for further research and therapeutic targeting.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [FABP1/3482] - BSA and Azide free (ab268176), expandable thumbnail](https://content.abcam.com/products/images/liver-fabp-antibody-fabp1-3482-bsa-and-azide-free-ab268176--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65436.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [FABP1/3482] - BSA and Azide free (ab268176)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [FABP1/3482] - BSA and Azide free (ab268176)
Formalin-fixed, paraffin-embedded human liver carcinoma tissue stained for liver FABP using Anti-liver FABP antibody [FABP1/3482] ab268055 at 2 μg/ml for 30 minutes at room temperature in immunohistochemical analysis.
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-liver FABP antibody [FABP1/3482] ab268055).
![Western blot - Anti-liver FABP antibody [FABP1/3482] - BSA and Azide free (ab268176), expandable thumbnail](https://content.abcam.com/products/images/liver-fabp-antibody-fabp1-3482-bsa-and-azide-free-ab268176--western-blot-img29034.jpg "Western blot - Anti-liver FABP antibody [FABP1/3482] - BSA and Azide free (ab268176)")
Western blot - Anti-liver FABP antibody [FABP1/3482] - BSA and Azide free (ab268176)
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-liver FABP antibody [FABP1/3482] ab268055).
All lanes: Western blot - Anti-liver FABP antibody [FABP1/3482] (Anti-liver FABP antibody [FABP1/3482] ab268055) at 2 µg/mL
All lanes: Human kidney tissue lysate
Predicted band size: 14 kDa
![Protein Array - Anti-liver FABP antibody [FABP1/3482] - BSA and Azide free (ab268176), expandable thumbnail](https://content.abcam.com/products/images/liver-fabp-antibody-fabp1-3482-bsa-and-azide-free-ab268176--protein-array-img140007.jpg "Protein Array - Anti-liver FABP antibody [FABP1/3482] - BSA and Azide free (ab268176)")
Protein Array - Anti-liver FABP antibody [FABP1/3482] - BSA and Azide free (ab268176)
Protein Array containing more than 19,000 full-length human proteins using Anti-liver FABP antibody [FABP1/3482] ab268055. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-liver FABP antibody [FABP1/3482] ab268055)
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