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AB7366

Anti-liver FABP antibody [L2B10]

4

(10 Reviews)

|

(19 Publications)

Anti-liver FABP antibody [L2B10] (ab7366) is a mouse monoclonal antibody detecting liver FABP in Western Blot, Flow Cytometry, IHC-P, IHC-Fr, FuncS. Suitable for African green monkey, Cow, Human, Mouse, Rat.

- Over 10 publications
- Trusted since 2002

View Alternative Names

FABPL, FABP1, Fatty acid-binding protein 1, Liver-type fatty acid-binding protein, L-FABP

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (AB7366)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (AB7366)

Immunohistochemistry (Paraffin-embedded sections) analysis of human kidney tissue labelling liver FABP with ab7366 at 1/1000. Antigen retrieval was performed using glycine buffer. Magnification : 200X.

Immunohistochemistry (Frozen sections) - Anti-liver FABP antibody [L2B10] (AB7366)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-liver FABP antibody [L2B10] (AB7366)

Immunohistochemical analysis of human L-FABP in frozen human kidney tissue with ab7366 at 1.9 μg/ml.

Flow Cytometry - Anti-liver FABP antibody [L2B10] (AB7366)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-liver FABP antibody [L2B10] (AB7366)

Overlay histogram showing HepG2 cells stained with ab110336 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110336, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (AB7366)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (AB7366)

Immunohistochemistry (Paraffin-embedded sections) analysis of human kidney tissue labelling liver FABP with ab7366 at 1/1000. Antigen retrieval was performed using glycine buffer. Magnification : 50X.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

L2B10

Isotype

IgG1

Carrier free

No

Reacts with

Human, African green monkey, Cow, Mouse, Rat

Applications

IHC-P, IHC-Fr, Flow Cyt, WB, FuncS

applications

Specificity

This monoclonal antibody binds to human liver FABP of both natural and recombinant origin.No reactivity to human intestinal or heart FABP.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FuncS" : {"fullname" : "Functional Studies", "shortname":"FuncS"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p>Liver FABP is a sensitive marker for cell damage of liver cells in vivo and useful for example for in vitro toxicology tests. Cytotoxicity protocol: * Culture 100 ml of 1.105 HUH-7 cells per ml in 96-wells plate. * T=0: Add 100 ml of 2x test chemicals to 96-wells plate. * Incubate 37 degree C, 5% CO2. * T=24: measure L-FABP in supernatant.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p>Liver FABP is a sensitive marker for cell damage of liver cells in vivo and useful for example for in vitro toxicology tests. Cytotoxicity protocol: * Culture 100 ml of 1.105 HUH-7 cells per ml in 96-wells plate. * T=0: Add 100 ml of 2x test chemicals to 96-wells plate. * Incubate 37 degree C, 5% CO2. * T=24: measure L-FABP in supernatant.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p>Liver FABP is a sensitive marker for cell damage of liver cells in vivo and useful for example for in vitro toxicology tests. Cytotoxicity protocol: * Culture 100 ml of 1.105 HUH-7 cells per ml in 96-wells plate. * T=0: Add 100 ml of 2x test chemicals to 96-wells plate. * Incubate 37 degree C, 5% CO2. * T=24: measure L-FABP in supernatant.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "African green monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p>Liver FABP is a sensitive marker for cell damage of liver cells in vivo and useful for example for in vitro toxicology tests. Cytotoxicity protocol: * Culture 100 ml of 1.105 HUH-7 cells per ml in 96-wells plate. * T=0: Add 100 ml of 2x test chemicals to 96-wells plate. * Incubate 37 degree C, 5% CO2. * T=24: measure L-FABP in supernatant.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p>Liver FABP is a sensitive marker for cell damage of liver cells in vivo and useful for example for in vitro toxicology tests. Cytotoxicity protocol: * Culture 100 ml of 1.105 HUH-7 cells per ml in 96-wells plate. * T=0: Add 100 ml of 2x test chemicals to 96-wells plate. * Incubate 37 degree C, 5% CO2. * T=24: measure L-FABP in supernatant.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Vervet monkey": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FuncS-species-checked": "notRecommended", "FuncS-species-dilution-info": "", "FuncS-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-liver FABP antibody [L2B10] (ab7366) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Functional Studies in African green monkey, Cow, Human, Mouse, Rat samples.

What is the molecular weight of liver FABP?
Anti-liver FABP [L2B10] (ab7366) specifically detects a band for liver FABP (UniProt: P07148) at a molecular weight of 14kDa.

Trusted by the scientific community
Anti-liver FABP [L2B10] (ab7366) was first used in a scientific publication in 2002 and has been cited over 10 times in peer-reviewed journals.

Reviewed by scientists
Anti-liver FABP [L2B10] (ab7366) has over 5 independent reviews from customers.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.1% Sodium azide Constituents: 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Liver fatty acid binding protein (liver FABP) also known as L-FABP or FABP1 is a small protein with a mass of approximately 14 kilodaltons. It functions mainly in the liver where it binds free fatty acids and other lipophilic substances facilitating their transport within cells. This protein is highly expressed in hepatocytes and also found in the small intestine and kidneys. Its role in binding fatty acids positions it as an important mediator in lipid metabolism making it of interest in a variety of metabolic studies.
Biological function summary

Liver FABP is essential for maintaining lipid homeostasis within cells. It is not part of a larger complex but acts by itself to regulate the intracellular concentration of lipids and protect cells from lipotoxicity. By sequestering fatty acids it prevents these molecules from disrupting cellular membranes or signaling pathways. Liver FABP also participates in the uptake transport and metabolic conversion of fatty acids and their metabolites influencing energy homeostasis and other vital processes.

Pathways

Liver FABP plays a significant role in the fatty acid metabolism and peroxisome proliferator-activated receptor (PPAR) signaling pathways. It acts by modulating the availability of lipid ligands necessary for PPAR activation linking it functionally to these nuclear receptors that control gene expression involved in lipid and glucose metabolism. Liver FABP's association with proteins like FABP2 and FABP4 within these pathways provides insight into its broader metabolic network highlighting its interactions in fatty acid transport and metabolism.

Liver FABP shows a strong connection to metabolic conditions particularly non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes. Its dysregulation is often observed in these disorders which include altered lipid profiles and insulin resistance. Liver FABP is also linked to certain forms of cancer with aberrant expression levels found in some tumor types. The interactions of liver FABP with proteins such as FABP5 in these diseases suggest a potential role in the pathogenesis of metabolic disorders and tumor development making it a candidate for further research and therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PubMed : 25732850). Binds cholesterol (PubMed : 25732850). Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport (By similarity).
See full target information FABP1
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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:540 PubMed40169914

2025

Functional maturation of preterm intestinal epithelium through CFTR activation.

Applications

Unspecified application

Species

Unspecified reactive species

Jihyun Kim,Hyunji Park,Na-Young Park,Se In Hwang,Young Eun Kim,Se In Sung,Yun Sil Chang,Ara Koh

Toxins 15: PubMed37505679

2023

Cyanotoxins Increase Cytotoxicity and Promote Nonalcoholic Fatty Liver Disease Progression by Enhancing Cell Steatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Suryakant Niture,Sashi Gadi,Qi Qi,Leslimar Rios-Colon,Sabin Khatiwada, Vandana,Reshan A Fernando,Keith E Levine,Deepak Kumar

Cancers 15: PubMed36980601

2023

MicroRNA-483-5p Inhibits Hepatocellular Carcinoma Cell Proliferation, Cell Steatosis, and Fibrosis by Targeting PPARα and TIMP2.

Applications

Unspecified application

Species

Unspecified reactive species

Suryakant Niture,Sashi Gadi,Qi Qi,Maxwell Afari Gyamfi,Rency S Varghese,Leslimar Rios-Colon,Uchechukwu Chimeh, Vandana,Habtom W Ressom,Deepak Kumar

PloS one 18:e0281414 PubMed36827398

2023

Ganglioside GM3 prevents high fat diet-induced hepatosteatosis via attenuated insulin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Orie Tajima,Yuki Fujita,Yuhsuke Ohmi,Koichi Furukawa,Keiko Furukawa

EMBO molecular medicine 13:e14123 PubMed34409732

2021

Mitogen-activated protein kinase activity drives cell trajectories in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Florian Uhlitz,Philip Bischoff,Stefan Peidli,Anja Sieber,Alexandra Trinks,Mareen Lüthen,Benedikt Obermayer,Eric Blanc,Yana Ruchiy,Thomas Sell,Soulafa Mamlouk,Roberto Arsie,Tzu-Ting Wei,Kathleen Klotz-Noack,Roland F Schwarz,Birgit Sawitzki,Carsten Kamphues,Dieter Beule,Markus Landthaler,Christine Sers,David Horst,Nils Blüthgen,Markus Morkel

Nutrients 13: PubMed34444927

2021

Decreased Fatty Acid Transporter FABP1 and Increased Isoprostanes and Neuroprostanes in the Human Term Placenta: Implications for Inflammation and Birth Weight in Maternal Pre-Gestational Obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Livia Belcastro,Carolina S Ferreira,Marcelle A Saraiva,Daniela B Mucci,Antonio Murgia,Carla Lai,Claire Vigor,Camille Oger,Jean-Marie Galano,Gabriela D A Pinto,Julian L Griffin,Alexandre G Torres,Thierry Durand,Graham J Burton,Fátima L C Sardinha,Tatiana El-Bacha

Cell death & disease 11:178 PubMed32152268

2020

TNFAIP8 regulates autophagy, cell steatosis, and promotes hepatocellular carcinoma cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Suryakant Niture,Maxwell A Gyamfi,Minghui Lin,Uchechukwu Chimeh,Xialan Dong,Weifan Zheng,John Moore,Deepak Kumar

Annals of hepato-biliary-pancreatic surgery 23:109-114 PubMed31225410

2019

Molecular classification of hepatocellular adenoma: A single-center experience.

Applications

Unspecified application

Species

Unspecified reactive species

Xue-Yin Shen,Xu-Guang Hu,Young-Bae Kim,Mi-Na Kim,Sung-Yeon Hong,Bong-Wan Kim,Hee-Jung Wang

PLoS biology 16:e2002417 PubMed29596476

2018

The colonic epithelium plays an active role in promoting colitis by shaping the tissue cytokine profile.

Applications

Unspecified application

Species

Unspecified reactive species

Jesse Lyons,Phaedra C Ghazi,Alina Starchenko,Alessio Tovaglieri,Katherine R Baldwin,Emily J Poulin,Jessica J Gierut,Casie Genetti,Vijay Yajnik,David T Breault,Douglas A Lauffenburger,Kevin M Haigis

Molecular and clinical oncology 5:267-270 PubMed27446562

2016

Histopathological characteristics of glutamine synthetase-positive hepatic tumor lesions in a mouse model of spontaneous metabolic syndrome (TSOD mouse).

Applications

IHC-P

Species

Mouse

Tetsuyuki Takahashi,Takeshi Nishida,Hayato Baba,Hideki Hatta,Johji Imura,Mitsuko Sutoh,Syunji Toyohara,Ryoji Hokao,Syunsuke Watanabe,Hirohisa Ogawa,Hisanori Uehara,Koichi Tsuneyama
View all publications
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Product promise

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