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AB244998

Anti-LKB1 antibody [EPR19490-176] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal LKB1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
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Sandwich ELISA - Anti-LKB1 antibody [EPR19490-176] - BSA and Azide free (Detector) (AB244998)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-LKB1 antibody [EPR19490-176] - BSA and Azide free (Detector) (AB244998)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab233612).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19490-176

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab244998 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human LKB1. The recommended pair for sandwich ELISA is:
Capture: ab244730, Human LKB1 Capture Antibody (unconjugated)
Detector: ab244998, Human LKB1 Detector Antibody (unconjugated)
The reference range value is 3.13 - 200 ng/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LKB1 also known as STK11 is a serine/threonine kinase with a molecular weight of approximately 48 kDa. This protein is widely expressed in various tissues including the liver pancreas and skeletal muscle. LKB1 plays an essential role in cellular energy homeostasis by phosphorylating and activating a group of at least 14 different proteins within the AMP-activated protein kinase (AMPK) family. The LKB1 protein acts as a master upstream kinase ensuring proper cellular energy balance and metabolism under conditions of nutrient stress.
Biological function summary

LKB1 is involved in controlling cell growth polarity and metabolism. It forms a complex with STRAD and MO25 which are necessary for its activation and function. By influencing these biological processes LKB1 regulates cell cycle arrest in response to stress signals and contributes to maintaining cellular integrity. The regulation of these processes positions LKB1 as an important factor in managing how cells respond to metabolic challenges.

Pathways

Its function integrates into major signaling networks like the AMPK and mTOR pathways. LKB1 activates AMPK which in turn affects mTOR signaling a pathway critical for cell growth and autophagy regulation. LKB1's interaction with AMPK links cellular energy levels to the control of biosynthetic pathways and cell cycle progression. The LKB1-AMPK-mTOR axis for instance highlights the protein's role in managing how cells balance growth-related activities and energy conservation.

LKB1 mutations or malfunctions are linked to Peutz-Jeghers syndrome and various forms of cancer such as lung cancer. Altered LKB1 activity affects its ability to suppress tumors primarily through its pathway interactions with AMPK and mTOR. Dysfunction in this pathway leads to unregulated cell growth and subsequently tumorigenesis. Understanding LKB1's role in these conditions aids in developing targeted therapies and diagnostic tools to manage associated risks and interventions effectively.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, thus promoting their activity : phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including : inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neuron polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response : interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1. Also acts as a mediator of p53/TP53-dependent apoptosis via interaction with p53/TP53 : translocates to the mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with NUAK1, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair (PubMed : 25329316).. Isoform 2. Has a role in spermiogenesis.
See full target information STK11

Alternative Names

LKB1, PJS, STK11, Serine/threonine-protein kinase STK11, Liver kinase B1, Renal carcinoma antigen NY-REN-19, hLKB1
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Product promise

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