Rabbit Polyclonal LKB1 phospho S428 antibody. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human STK11 phospho S428.
Images
Publications
Filter by
- Bioengineered 13:13667-136792022lncRNA NBR2 attenuates angiotensin II-induced myocardial hypertrophy through repressing ER stress via activating LKB1/AMPK/Sirt1 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCansheng Zhu,Min Wang,Xianguan Yu,Xing Shui,Leile Tang,Zefeng Chen,Zhaojun XiongPubMed 35703318
- Bioengineered 13:12409-124202022Wnt family member 1 (Wnt1) overexpression-induced M2 polarization of microglia alleviates inflammation-sensitized neonatal brain injuries.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinzhi Gao,Hao Xu,Zhihui Rong,Ling ChenPubMed 35603707
- Frontiers in pharmacology 12:7549762021Ferulic Acid Ameliorates Hepatic Inflammation and Fibrotic Liver Injury by Inhibiting PTP1B Activity and Subsequent Promoting AMPK Phosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianzhi Wu,Xiaoyong Xue,Guifang Fan,Yiqing Gu,Fei Zhou,Qi Zheng,Runping Liu,Yajing Li,Boning Ma,Shuo Li,Guangrui Huang,Lin Ma,Xiaojiaoyang LiPubMed 34566665
- Cell biology international 45:305-3192020PSPH induces cell autophagy and promotes cell proliferation and invasion in the hepatocellular carcinoma cell line Huh7 via the AMPK/mTOR/ULK1 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianli Zhang,Erhao Wang,Lei Zhang,Bo ZhouPubMed 33079432
- Nature medicine 25:620-6272019Protective autophagy elicited by RAF→MEK→ERK inhibition suggests a treatment strategy for RAS-driven cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesConan G Kinsey et. al.PubMed 30833748
- Cardiovascular research 114:1738-17512018PCSK9 expression in the ischaemic heart and its relationship to infarct size, cardiac function, and development of autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZufeng Ding,Xianwei Wang,Shijie Liu,Jiwani Shahanawaz,Sue Theus,Yubo Fan,Xiaoyan Deng,Sichang Zhou,Jawahar L MehtaPubMed 29800228
- Molecular medicine reports 17:2837-28442017Activation of the LKB1‑SIK1 signaling pathway inhibits the TGF‑β‑mediated epithelial‑mesenchymal transition and apoptosis resistance of ovarian carcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Hong,Jianmei Zhang,Wenlan YangPubMed 29257268
- Scientific reports 6:189492016BRAF V600E inhibition stimulates AMP-activated protein kinase-mediated autophagy in colorectal cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesToshinori Sueda et. al.PubMed 26750638
- Oncotarget 6:23857-732015Lovastatin overcomes gefitinib resistance through TNF-α signaling in human cholangiocarcinomas with different LKB1 statuses in vitro and in vivo.Applications:IHC, WBReactive species:Unspecified reactive speciesSheng-Huei Yang,Hung-Yun Lin,Vincent H S Chang,Chien-Chung Chen,Yun-Ru Liu,Jinghan Wang,Keqiang Zhang,Xiaoqing Jiang,Yun YenPubMed 26160843
- Cell 155:688-982013Cyclic dinucleotides trigger ULK1 (ATG1) phosphorylation of STING to prevent sustained innate immune signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroyasu Konno et. al.PubMed 24119841
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human STK11 phospho S428. The exact immunogen used to generate this antibody is proprietary information. Database link Q15831
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Mouse | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, thus promoting their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neuron polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1. Also acts as a mediator of p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to the mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with NUAK1, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair (PubMed:25329316). Isoform 2. Has a role in spermiogenesis.
Alternative names
LKB1, PJS, STK11, Serine/threonine-protein kinase STK11, Liver kinase B1, Renal carcinoma antigen NY-REN-19, hLKB1
Recommended products
Rabbit Polyclonal LKB1 phospho S428 antibody. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human STK11 phospho S428.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human STK11 phospho S428. The exact immunogen used to generate this antibody is proprietary information. Database link Q15831
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab63473 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LKB1 also known as STK11 is a serine/threonine kinase with a molecular weight of approximately 48 kDa. This protein is widely expressed in various tissues including the liver pancreas and skeletal muscle. LKB1 plays an essential role in cellular energy homeostasis by phosphorylating and activating a group of at least 14 different proteins within the AMP-activated protein kinase (AMPK) family. The LKB1 protein acts as a master upstream kinase ensuring proper cellular energy balance and metabolism under conditions of nutrient stress.
- Biological function summary
LKB1 is involved in controlling cell growth polarity and metabolism. It forms a complex with STRAD and MO25 which are necessary for its activation and function. By influencing these biological processes LKB1 regulates cell cycle arrest in response to stress signals and contributes to maintaining cellular integrity. The regulation of these processes positions LKB1 as an important factor in managing how cells respond to metabolic challenges.
- Pathways
Its function integrates into major signaling networks like the AMPK and mTOR pathways. LKB1 activates AMPK which in turn affects mTOR signaling a pathway critical for cell growth and autophagy regulation. LKB1's interaction with AMPK links cellular energy levels to the control of biosynthetic pathways and cell cycle progression. The LKB1-AMPK-mTOR axis for instance highlights the protein's role in managing how cells balance growth-related activities and energy conservation.
- Associated diseases and disorders
LKB1 mutations or malfunctions are linked to Peutz-Jeghers syndrome and various forms of cancer such as lung cancer. Altered LKB1 activity affects its ability to suppress tumors primarily through its pathway interactions with AMPK and mTOR. Dysfunction in this pathway leads to unregulated cell growth and subsequently tumorigenesis. Understanding LKB1's role in these conditions aids in developing targeted therapies and diagnostic tools to manage associated risks and interventions effectively.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-LKB1 (phospho S428) antibody (ab63473)
All lanes: Western blot - Anti-LKB1 (phospho S428) antibody (ab63473) at 1/500 dilution
Lane 1: mouse brain extracts at 30 µg
Lane 2: mouse brain extracts at 30 µg with immunising peptide
Predicted band size: 48 kDa
Observed band size: 45 kDa, 48 kDa
Immunocytochemistry/ Immunofluorescence - Anti-LKB1 (phospho S428) antibody (ab63473)
ab63473 at 1/500 dilution staining LKB1 in NIH3T3 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com