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AB32583

Anti-LMAN1 antibody

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(1 Publication)

Rabbit Polyclonal LMAN1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Rat Lman1 aa 150-200.

View Alternative Names

ERGIC53, F5F8D, LMAN1, Protein ERGIC-53, ER-Golgi intermediate compartment 53 kDa protein, Gp58, Intracellular mannose-specific lectin MR60, Lectin mannose-binding 1

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-LMAN1 antibody (AB32583)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LMAN1 antibody (AB32583)

ab32583 labelling LMAN1 in HMVEC cells by Immunocytochemistry/Immunofluorescence.

Immunocytochemistry/ Immunofluorescence - Anti-LMAN1 antibody (AB32583)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LMAN1 antibody (AB32583)

ab32583 labelling LMAN1 in A549 cells by Immunocytochemistry/Immunofluorescence.

Immunocytochemistry/ Immunofluorescence - Anti-LMAN1 antibody (AB32583)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LMAN1 antibody (AB32583)

ab32583 labelling LMAN1 in p19 cells by Immunocytochemistry/Immunofluorescence.

Western blot - Anti-LMAN1 antibody (AB32583)
  • WB

Unknown

Western blot - Anti-LMAN1 antibody (AB32583)

All lanes:

Western blot - Anti-LMAN1 antibody (ab32583) at 1/1000 dilution

Lane 1:

Rat brain lysate

Lane 2:

Rat brain lysate with Immunising peptide

Predicted band size: 58 kDa

Observed band size: 57.5 kDa

false

Western blot - Anti-LMAN1 antibody (AB32583)
  • WB

Supplier Data

Western blot - Anti-LMAN1 antibody (AB32583)

All lanes:

Western blot - Anti-LMAN1 antibody (ab32583) at 1/2000 dilution

Lane 1:

HepG2 lysate at 30 µg

Lane 2:

HeLa lysate at 30 µg

Lane 3:

PANC-1 lysate at 30 µg

Lane 4:

MCF7 lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human, Mouse

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Rat Lman1 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

Q62902

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LMAN1 also known as ERGIC-53 or lectin mannose-binding 1 is a protein involved in the transport and quality control of glycoproteins. It has a mass of approximately 53 kDa. LMAN1 primarily expresses in the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and is important for efficient cargo sorting and proper folding. It functions by binding to specific carbohydrate moieties on glycoproteins aiding their transport between the ER and Golgi apparatus.
Biological function summary

LMAN1 functions significantly within a protein complex known as the LMAN1-MCFD2 complex. It serves an essential role as a cargo receptor for glycoproteins particularly those with particular mannose-rich domains influencing their secretion and stability. This protein complex is integral in maintaining protein homeostasis and cellular physiology by ensuring the correct sorting and transport of glycoproteins from the ER to the Golgi.

Pathways

LMAN1 significantly participates in the secretory pathway and quality control mechanisms in the cell. It interacts with proteins such as MCFD2 to mediate the transport of specific glycoproteins like α1-antitrypsin through the secretory pathway. This pathway is important for the secretion of a variety of proteins and disruptions can lead to disorders associated with improper protein folding and trafficking.

Defects in LMAN1 can lead to bleeding disorders such as combined coagulation factor V and VIII deficiency. The protein's dysfunction impairs the transport of coagulation factors disrupting their secretion. Additionally proteins like MCFD2 work closely with LMAN1 in this context as mutations in either protein can cause a similar deficiency highlighting the significant role of LMAN1 in maintaining normal hemostatic processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.
See full target information LMAN1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 7: PubMed29334356

2018

p53 orchestrates DNA replication restart homeostasis by suppressing mutagenic RAD52 and POLθ pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Sunetra Roy,Karl-Heinz Tomaszowski,Jessica W Luzwick,Soyoung Park,Jun Li,Maureen Murphy,Katharina Schlacher
View all publications
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Product promise

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