AB118407

Anti-LMAN1 antibody [OTI1B8]

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(2 Publications)

Mouse Monoclonal LMAN1 antibody. Suitable for WB and reacts with Human, Transfected cell lysate, African green monkey, Dog, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LMAN1.

View Alternative Names

ERGIC53, F5F8D, LMAN1, Protein ERGIC-53, ER-Golgi intermediate compartment 53 kDa protein, Gp58, Intracellular mannose-specific lectin MR60, Lectin mannose-binding 1

4 Images

Lab

Western blot - Anti-LMAN1 antibody [OTI1B8] (AB118407)

Lanes 1 - 4 : Merged signal (red and green). Green - ab118407 observed at 55 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.

ab118407 was shown to react with LMAN1 in wild-type HEK-293T cells in western blot with loss of signal observed in LMAN1 knockout cell line ab266248 (LMAN1 knockout cell lysate ab257505). Wild-type and LMAN1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab118407 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 200 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-LMAN1 antibody [OTI1B8] (ab118407) at 1/200 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

LMAN1 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human LMAN1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-lman1-knockout-hek-293t-cell-line-ab266248'>ab266248</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Predicted band size: 58 kDa

Observed band size: 55 kDa

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Supplier Data

Western blot - Anti-LMAN1 antibody [OTI1B8] (AB118407)

Lanes 1 - 2:

Western blot - Anti-LMAN1 antibody [OTI1B8] (ab118407) at 1/1000 dilution

Lanes 3 - 4:

anti-HSP90AB1

Lanes 1 and 3:

Wild-type HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 10 µg

Lanes 2 and 4:

LMAN1-knockout HEK-293T cell lysate at 10 µg

Predicted band size: 58 kDa

false

Unknown

Western blot - Anti-LMAN1 antibody [OTI1B8] (AB118407)

All lanes:

Western blot - Anti-LMAN1 antibody [OTI1B8] (ab118407) at 1/200 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell extract at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg

Lane 3:

HT29 (human colorectal adenocarcinoma cell line) cell extract at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) cell extract at 35 µg

Lane 5:

COS-7 (african green monkey kidney fibroblast-like cell line) cell extract at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) cell extract at 35 µg

Lane 7:

MDCK (canine kidney cell line) cell extract at 35 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg

Lane 9:

MCF7 (human breast adenocarcinoma cell line) cell extract at 35 µg

Predicted band size: 58 kDa

false

Unknown

Western blot - Anti-LMAN1 antibody [OTI1B8] (AB118407)

All lanes:

Western blot - Anti-LMAN1 antibody [OTI1B8] (ab118407) at 1/200 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY LMAN1 cDNA at 5 µg

Predicted band size: 58 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1B8

Isotype

IgG1

Carrier free

Reacts with

Rat, Dog, Human, African green monkey

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human LMAN1.

P49257

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/500", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/500", "WB-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/500", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/500", "WB-species-notes": "" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/500", "WB-species-notes": "" } } }

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Product details

Clone OTI1B8 (formerly 1B8).

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from cell culture supernatant by affinity chromatography..

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LMAN1 also known as ERGIC-53 or lectin mannose-binding 1 is a protein involved in the transport and quality control of glycoproteins. It has a mass of approximately 53 kDa. LMAN1 primarily expresses in the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and is important for efficient cargo sorting and proper folding. It functions by binding to specific carbohydrate moieties on glycoproteins aiding their transport between the ER and Golgi apparatus.

Biological function summary

LMAN1 functions significantly within a protein complex known as the LMAN1-MCFD2 complex. It serves an essential role as a cargo receptor for glycoproteins particularly those with particular mannose-rich domains influencing their secretion and stability. This protein complex is integral in maintaining protein homeostasis and cellular physiology by ensuring the correct sorting and transport of glycoproteins from the ER to the Golgi.

Pathways

LMAN1 significantly participates in the secretory pathway and quality control mechanisms in the cell. It interacts with proteins such as MCFD2 to mediate the transport of specific glycoproteins like α1-antitrypsin through the secretory pathway. This pathway is important for the secretion of a variety of proteins and disruptions can lead to disorders associated with improper protein folding and trafficking.

Defects in LMAN1 can lead to bleeding disorders such as combined coagulation factor V and VIII deficiency. The protein's dysfunction impairs the transport of coagulation factors disrupting their secretion. Additionally proteins like MCFD2 work closely with LMAN1 in this context as mutations in either protein can cause a similar deficiency highlighting the significant role of LMAN1 in maintaining normal hemostatic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.

See full target information LMAN1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 27:271-287 PubMed37122901

2023

Cardiac patches made of brown adipose-derived stem cell sheets and conductive electrospun nanofibers restore infarcted heart for ischemic myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Xinbo Wei,Li Wang,Cuimi Duan,Kai Chen,Xia Li,Ximin Guo,Peng Chen,Haifeng Liu,Yubo Fan

Journal of virology 94: PubMed32554695

2020

Porcine Epidemic Diarrhea Virus ORF3 Protein Is Transported through the Exocytic Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fusheng Si,Bingqing Chen,Xiaoxia Hu,Ruisong Yu,Shijuan Dong,Ruiyang Wang,Zhen Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LMAN1 antibody [OTI1B8]

Western blot - Anti-LMAN1 antibody [OTI1B8] (AB118407)

Western blot - Anti-LMAN1 antibody [OTI1B8] (AB118407)

Western blot - Anti-LMAN1 antibody [OTI1B8] (AB118407)

Western blot - Anti-LMAN1 antibody [OTI1B8] (AB118407)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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