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AB249507

Anti-LMCD1 antibody [EPR11443(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal LMCD1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

LIM and cysteine-rich domains protein 1, Dyxin, LMCD1

2 Images
Immunoprecipitation - Anti-LMCD1 antibody [EPR11443(B)] - BSA and Azide free (AB249507)
  • IP

Supplier Data

Immunoprecipitation - Anti-LMCD1 antibody [EPR11443(B)] - BSA and Azide free (AB249507)

This data was developed using ab169760, the same antibody clone in a different buffer formulation.

Detection of LMCD1 by Western Blot of Immunprecipitate.

Human skeletal muscle lysate immunoprecipitated using ab169760 at 1/10 dilution (lane 1) or a 1 X PBS negative control (lane 2) and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-LMCD1 antibody [EPR11443(B)] (<a href='/en-us/products/primary-antibodies/lmcd1-antibody-epr11443b-ab169760'>ab169760</a>)

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

Western blot - Anti-LMCD1 antibody [EPR11443(B)] - BSA and Azide free (AB249507)
  • WB

Supplier Data

Western blot - Anti-LMCD1 antibody [EPR11443(B)] - BSA and Azide free (AB249507)

This data was developed using ab169760, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LMCD1 antibody [EPR11443(B)] (<a href='/en-us/products/primary-antibodies/lmcd1-antibody-epr11443b-ab169760'>ab169760</a>) at 1/1000 dilution

Lane 1:

Human skeletal muscle lysate at 10 µg

Lane 2:

SH-SY5Y lysate at 10 µg

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11443(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab249507 is the carrier-free version of ab169760.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LMCD1 also known as LIM and cysteine-rich domains protein 1 is a protein that plays several roles at the cell level. This protein has a mass of approximately 39 kDa. LMCD1 is expressed in various tissues in the body including the heart and liver. It contains LIM domains which are important for protein-protein interactions and cysteine-rich domains which contribute to its function.
Biological function summary

LMCD1 regulates gene expression by acting as a transcriptional co-regulator. It is not part of a large complex but interacts with other proteins to modulate cardiac and hepatic functions. By affecting transcription LMCD1 influences development and maintenance of specific tissue structures including heart muscle and liver tissues.

Pathways

LMCD1 plays a role in the regulation of the Wnt signaling pathway vital for cardiac and hepatic development. It interacts with beta-catenin a central protein in this pathway impacting cell growth and differentiation. LMCD1 also has connections to the TGF-beta signaling pathway where it associates with Smad proteins that mediate cellular responses.

LMCD1 links to cardiac hypertrophy and hepatocellular carcinoma. In cardiac hypertrophy it interacts with GATA4 a transcription factor involved in heart muscle growth. In hepatocellular carcinoma changes in LMCD1 expression can disrupt normal cell proliferation by altering TGF-beta signaling affecting several downstream targets including SMAD proteins.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity). Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T-cells signaling pathway.
See full target information LMCD1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com