Rabbit Recombinant Monoclonal LMCD1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Images
![Immunoprecipitation - Anti-LMCD1 antibody [EPR11443(B)] - BSA and Azide free (AB249507), expandable thumbnail](https://content.abcam.com/products/images/lmcd1-antibody-epr11443b-bsa-and-azide-free-ab249507--immunoprecipitation-img73507.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-LMCD1 antibody [EPR11443(B)] - BSA and Azide free (AB249507)")
![Western blot - Anti-LMCD1 antibody [EPR11443(B)] - BSA and Azide free (AB249507), expandable thumbnail](https://content.abcam.com/products/images/lmcd1-antibody-epr11443b-bsa-and-azide-free-ab249507--western-blot-img57333.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LMCD1 antibody [EPR11443(B)] - BSA and Azide free (AB249507)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity). Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T-cells signaling pathway.
Alternative names
LIM and cysteine-rich domains protein 1, Dyxin, LMCD1
Recommended products
Rabbit Recombinant Monoclonal LMCD1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR11443(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249507 is the carrier-free version of Anti-LMCD1 antibody [EPR11443(B)] ab169760.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LMCD1 also known as LIM and cysteine-rich domains protein 1 is a protein that plays several roles at the cell level. This protein has a mass of approximately 39 kDa. LMCD1 is expressed in various tissues in the body including the heart and liver. It contains LIM domains which are important for protein-protein interactions and cysteine-rich domains which contribute to its function.
- Biological function summary
LMCD1 regulates gene expression by acting as a transcriptional co-regulator. It is not part of a large complex but interacts with other proteins to modulate cardiac and hepatic functions. By affecting transcription LMCD1 influences development and maintenance of specific tissue structures including heart muscle and liver tissues.
- Pathways
LMCD1 plays a role in the regulation of the Wnt signaling pathway vital for cardiac and hepatic development. It interacts with beta-catenin a central protein in this pathway impacting cell growth and differentiation. LMCD1 also has connections to the TGF-beta signaling pathway where it associates with Smad proteins that mediate cellular responses.
- Associated diseases and disorders
LMCD1 links to cardiac hypertrophy and hepatocellular carcinoma. In cardiac hypertrophy it interacts with GATA4 a transcription factor involved in heart muscle growth. In hepatocellular carcinoma changes in LMCD1 expression can disrupt normal cell proliferation by altering TGF-beta signaling affecting several downstream targets including SMAD proteins.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunoprecipitation - Anti-LMCD1 antibody [EPR11443(B)] - BSA and Azide free (ab249507), expandable thumbnail](https://content.abcam.com/products/images/lmcd1-antibody-epr11443b-bsa-and-azide-free-ab249507--immunoprecipitation-img73507.jpg "Immunoprecipitation - Anti-LMCD1 antibody [EPR11443(B)] - BSA and Azide free (ab249507)")
Immunoprecipitation - Anti-LMCD1 antibody [EPR11443(B)] - BSA and Azide free (ab249507)
This data was developed using Anti-LMCD1 antibody [EPR11443(B)] ab169760, the same antibody clone in a different buffer formulation.
Detection of LMCD1 by Western Blot of Immunprecipitate.
Human skeletal muscle lysate immunoprecipitated using Anti-LMCD1 antibody [EPR11443(B)] ab169760 at 1/10 dilution (lane 1) or a 1 X PBS negative control (lane 2) and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes: Immunoprecipitation - Anti-LMCD1 antibody [EPR11443(B)] (Anti-LMCD1 antibody [EPR11443(B)] ab169760)
Predicted band size: 41 kDa
Observed band size: 41 kDa
![Western blot - Anti-LMCD1 antibody [EPR11443(B)] - BSA and Azide free (ab249507), expandable thumbnail](https://content.abcam.com/products/images/lmcd1-antibody-epr11443b-bsa-and-azide-free-ab249507--western-blot-img57333.jpg "Western blot - Anti-LMCD1 antibody [EPR11443(B)] - BSA and Azide free (ab249507)")
Western blot - Anti-LMCD1 antibody [EPR11443(B)] - BSA and Azide free (ab249507)
This data was developed using Anti-LMCD1 antibody [EPR11443(B)] ab169760, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-LMCD1 antibody [EPR11443(B)] (Anti-LMCD1 antibody [EPR11443(B)] ab169760) at 1/1000 dilution
Lane 1: Human skeletal muscle lysate at 10 µg
Lane 2: SH-SY5Y lysate at 10 µg
Predicted band size: 41 kDa
Downloads
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