Anti-LMO 4 antibody [20F8] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO 4 antibody [20F8] - BSA and Azide free (AB255746)

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling LMO 4 with ab243139 at 1/1000 dilution, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on mouse hippocampus tissue is observed. The section was incubated with ab243139 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243139).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO 4 antibody [20F8] - BSA and Azide free (AB255746)

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling LMO 4 with ab243139 at 1/1000 dilution, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on rat hippocampus tissue is observed. The section was incubated with ab243139 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243139).

• WB

Supplier Data

Western blot - Anti-LMO 4 antibody [20F8] - BSA and Azide free (AB255746)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1,2 : 15 secs; Lane 3 : 3 secs

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243139).

All lanes:

Western blot - Anti-LMO 4 antibody [20F8] - BSA and Azide free (ab255746) at 1.354 µg/mL

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate at 20 µg

Lane 3:

Mouse embryo tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution

false