AB91652

Anti-LMO2 antibody [EP3257]

RabMAb
Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal LMO2 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

RBTN2, RBTNL1, RHOM2, TTG2, LMO2, Rhombotin-2, Cysteine-rich protein TTG-2, LIM domain only protein 2, T-cell translocation protein 2, LMO-2

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Immunoprecipitation - Anti-LMO2 antibody [EP3257] (AB91652)

ab91652 (purified) at 1 : 30 dilution (2μg) immunoprecipitating LMO2 in Raji whole cell lysate.
Lane 1 (input) : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10μg
Lane 2 (+) : ab91652 & Raji whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab91652 in Raji whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-LMO2 antibody [EP3257] (ab91652)

Predicted band size: 18 kDa

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Western blot - Anti-LMO2 antibody [EP3257] (AB91652)

All lanes:

Western blot - Anti-LMO2 antibody [EP3257] (ab91652) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

Fetal kidney lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 18 kDa

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Western blot - Anti-LMO2 antibody [EP3257] (AB91652)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-LMO2 antibody [EP3257] (ab91652) at 0.3 µg/mL

Lane 1:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 15 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg

Lane 3:

Mouse E14 embryo tissue lysates at 15 µg

Lane 4:

Rat E14 embryo tissue lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 18 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP3257

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "For unpurified use at 1/100 - 1/500", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LMO2 also known as LIM Domain Only 2 is a transcription factor with a molecular weight of approximately 24 kDa. It contains two LIM domains which enable protein-protein interactions. LMO2 expression is mostly found in hematopoietic tissues and the vascular endothelium. This protein does not directly bind DNA but forms complexes with other proteins to exert its regulatory roles.

Biological function summary

This transcription factor is important in hematopoiesis and angiogenesis. LMO2 forms multiprotein complexes that include TAL1 GATA1 and E47 which are necessary for the transcriptional activation of genes involved in blood cell differentiation and formation. Its role in angiogenesis involves participating in the transcriptional regulation of genes essential for blood vessel formation.

Pathways

LMO2 plays a significant role in the development of the hematopoietic system and vascular network. It is part of the TAL1-LMO2-LDB1 transcriptional complex within the hematopoietic transcriptional network which includes other key proteins like GATA1 and E47. These pathways ensure proper development and differentiation of various blood cell lineages and endothelial cells.

LMO2 has a known association with T-cell acute lymphoblastic leukemia (T-ALL) and some other hematological malignancies. Abnormal expression of LMO2 can lead to oncogenic transformation in hematopoietic cells. Within these disease contexts LMO2 interacts with proteins like TAL1 and LDB1 contributing to leukemogenic processes. Understanding its role helps in the development of therapeutic strategies targeting these pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state.

See full target information LMO2

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 57:1457-1468 PubMed39757769

2025

MYB represses ζ-globin expression through upregulating ETO2.

Applications

Unspecified application

Species

Unspecified reactive species

Zejun Dong,Yuhua Ye,Wei Zhang,Hualei Luo,Jialong Li,Qianqian Zhang,Xinhua Zhang,Xiang Guo,Xiangmin Xu

Cell death & disease 14:518 PubMed37573405

2023

LMO2 promotes the development of AML through interaction with transcription co-regulator LDB1.

Applications

Unspecified application

Species

Unspecified reactive species

Lihui Lu,Jianwei Wang,Fang Fang,Ailian Guo,Shuting Jiang,Yanfang Tao,Yongping Zhang,Yan Li,Kunlong Zhang,Zimu Zhang,Ran Zhuo,Xinran Chu,Xiaolu Li,Yuanyuan Tian,Li Ma,Xu Sang,Yanling Chen,Juanjuan Yu,Yang Yang,Haibo Cao,Jizhao Gao,Jun Lu,Shaoyan Hu,Jian Pan,Hailong He

Science advances 8:eabm3548 PubMed36351009

2022

Identification of a minority population of LMO2 breast cancer cells that integrate into the vasculature and initiate metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Shaheen S Sikandar,Gunsagar S Gulati,Jane Antony,Isobel Fetter,Angera H Kuo,William Hai Dang Ho,Veronica Haro-Acosta,Soumyashree Das,Chloé B Steen,Thiago Almeida Pereira,Dalong Qian,Philip A Beachy,Frederick M Dirbas,Kristy Red-Horse,Terence H Rabbitts,Jean Paul Thiery,Aaron M Newman,Michael F Clarke

eLife 10: PubMed34382935

2021

LMO2 is essential to maintain the ability of progenitors to differentiate into T-cell lineage in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ken-Ichi Hirano,Hiroyuki Hosokawa,Maria Koizumi,Yusuke Endo,Takashi Yahata,Kiyoshi Ando,Katsuto Hozumi

eLife 7: PubMed29555020

2018

Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Isabelle Bergiers,Tallulah Andrews,Özge Vargel Bölükbaşı,Andreas Buness,Ewa Janosz,Natalia Lopez-Anguita,Kerstin Ganter,Kinga Kosim,Cemre Celen,Gülce Itır Perçin,Paul Collier,Bianka Baying,Vladimir Benes,Martin Hemberg,Christophe Lancrin

Medical science monitor : international medical journal of experimental and clinical research 23:695-703 PubMed28170369

2017

LMO2 Enhances Lamellipodia/Filopodia Formation in Basal-Type Breast Cancer Cells by Mediating ARP3-Profilin1 Interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Liu,Chao Wu,Tianhui Zhu,Wei Sun

View all publications

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