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AB247530

Anti-LMO2 antibody [EP3257] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal LMO2 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

RBTN2, RBTNL1, RHOM2, TTG2, LMO2, Rhombotin-2, Cysteine-rich protein TTG-2, LIM domain only protein 2, T-cell translocation protein 2, LMO-2

3 Images
Immunoprecipitation - Anti-LMO2 antibody [EP3257] - BSA and Azide free (AB247530)
  • IP

Unknown

Immunoprecipitation - Anti-LMO2 antibody [EP3257] - BSA and Azide free (AB247530)

This data was developed using ab91652, the same antibody clone in a different buffer formulation.

ab91652 (purified) at 1 : 30 dilution (2μg) immunoprecipitating LMO2 in Raji whole cell lysate.
Lane 1 (input) : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10μg
Lane 2 (+) : ab91652 & Raji whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab91652 in Raji whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-LMO2 antibody [EP3257] (<a href='/en-us/products/primary-antibodies/lmo2-antibody-ep3257-ab91652'>ab91652</a>)

Predicted band size: 18 kDa

false

Western blot - Anti-LMO2 antibody [EP3257] - BSA and Azide free (AB247530)
  • WB

Unknown

Western blot - Anti-LMO2 antibody [EP3257] - BSA and Azide free (AB247530)

This data was developed using ab91652, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LMO2 antibody [EP3257] (<a href='/en-us/products/primary-antibodies/lmo2-antibody-ep3257-ab91652'>ab91652</a>) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

Fetal kidney lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 18 kDa

false

Western blot - Anti-LMO2 antibody [EP3257] - BSA and Azide free (AB247530)
  • WB

Unknown

Western blot - Anti-LMO2 antibody [EP3257] - BSA and Azide free (AB247530)

This data was developed using ab91652, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-LMO2 antibody [EP3257] (<a href='/en-us/products/primary-antibodies/lmo2-antibody-ep3257-ab91652'>ab91652</a>) at 0.3 µg/mL

Lane 1:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 15 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg

Lane 3:

Mouse E14 embryo tissue lysates at 15 µg

Lane 4:

Rat E14 embryo tissue lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 18 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP3257

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab247530 is the carrier-free version of ab91652.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LMO2 also known as LIM Domain Only 2 is a transcription factor with a molecular weight of approximately 24 kDa. It contains two LIM domains which enable protein-protein interactions. LMO2 expression is mostly found in hematopoietic tissues and the vascular endothelium. This protein does not directly bind DNA but forms complexes with other proteins to exert its regulatory roles.
Biological function summary

This transcription factor is important in hematopoiesis and angiogenesis. LMO2 forms multiprotein complexes that include TAL1 GATA1 and E47 which are necessary for the transcriptional activation of genes involved in blood cell differentiation and formation. Its role in angiogenesis involves participating in the transcriptional regulation of genes essential for blood vessel formation.

Pathways

LMO2 plays a significant role in the development of the hematopoietic system and vascular network. It is part of the TAL1-LMO2-LDB1 transcriptional complex within the hematopoietic transcriptional network which includes other key proteins like GATA1 and E47. These pathways ensure proper development and differentiation of various blood cell lineages and endothelial cells.

LMO2 has a known association with T-cell acute lymphoblastic leukemia (T-ALL) and some other hematological malignancies. Abnormal expression of LMO2 can lead to oncogenic transformation in hematopoietic cells. Within these disease contexts LMO2 interacts with proteins like TAL1 and LDB1 contributing to leukemogenic processes. Understanding its role helps in the development of therapeutic strategies targeting these pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state.
See full target information LMO2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Obesity (Silver Spring, Md.) 30:666-679 PubMed35170865

2022

Exosomal miR-122 promotes adipogenesis and aggravates obesity through the VDR/SREBF1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Yan Huang,Ji-Xiong Chen,Yi Ren,Li-Chun Fan,Wei Xiang,Xiao-Jie He
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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