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AB308022

Anti-LMO2 antibody [RP23040039]

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Rabbit Recombinant Multiclonal LMO2 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human LMO2.

View Alternative Names

RBTN2, RBTNL1, RHOM2, TTG2, LMO2, Rhombotin-2, Cysteine-rich protein TTG-2, LIM domain only protein 2, T-cell translocation protein 2, LMO-2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-LMO2 antibody [RP23040039] (AB308022)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LMO2 antibody [RP23040039] (AB308022)

Immunofluorescent analysis of LMO2 in U2OS cells using ab308022 (1-3 µg/mL) followed by detection using an Alexa Fluor® 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (blue) (Image B). Actin stained with Alexa Fluor® 594 phalloidin (red) (image C). Image D is a composite image showing nuclear localization of LMO2.

Western blot - Anti-LMO2 antibody [RP23040039] (AB308022)
  • WB

Supplier Data

Western blot - Anti-LMO2 antibody [RP23040039] (AB308022)

Western blot analysis of LMO2 in HEK293 whole cell extracts using ab308022 at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~18kDa.

All lanes:

Western blot - Anti-LMO2 antibody [RP23040039] (ab308022) at 2 µg/mL

All lanes:

HEK293 whole cell lysate

Predicted band size: 18 kDa

Observed band size: 18 kDa

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Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040039

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human LMO2.

P25791

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-3 µg/mL", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LMO2 also known as LIM Domain Only 2 is a transcription factor with a molecular weight of approximately 24 kDa. It contains two LIM domains which enable protein-protein interactions. LMO2 expression is mostly found in hematopoietic tissues and the vascular endothelium. This protein does not directly bind DNA but forms complexes with other proteins to exert its regulatory roles.
Biological function summary

This transcription factor is important in hematopoiesis and angiogenesis. LMO2 forms multiprotein complexes that include TAL1 GATA1 and E47 which are necessary for the transcriptional activation of genes involved in blood cell differentiation and formation. Its role in angiogenesis involves participating in the transcriptional regulation of genes essential for blood vessel formation.

Pathways

LMO2 plays a significant role in the development of the hematopoietic system and vascular network. It is part of the TAL1-LMO2-LDB1 transcriptional complex within the hematopoietic transcriptional network which includes other key proteins like GATA1 and E47. These pathways ensure proper development and differentiation of various blood cell lineages and endothelial cells.

LMO2 has a known association with T-cell acute lymphoblastic leukemia (T-ALL) and some other hematological malignancies. Abnormal expression of LMO2 can lead to oncogenic transformation in hematopoietic cells. Within these disease contexts LMO2 interacts with proteins like TAL1 and LDB1 contributing to leukemogenic processes. Understanding its role helps in the development of therapeutic strategies targeting these pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state.
See full target information LMO2
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Product promise

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For full details, please see our Terms & Conditions

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