AB229226

Anti-LMO4 antibody

Name: Anti-LMO4 antibody (ab229226) | Abcam
Brand: Abcam Limited
SKU: AB229226
Rating: 3 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal LMO4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LMO4.

View Alternative Names

LIM domain transcription factor LMO4, Breast tumor autoantigen, LIM domain only protein 4, LMO-4, LMO4

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO4 antibody (AB229226)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO4 antibody (AB229226)

Paraffin-embedded human breast carcinoma tissue stained for LMO4 using ab229226 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-LMO4 antibody (AB229226)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-LMO4 antibody (ab229226) at 1/5000 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

LMO4-transfected HEK-293T whole cell extract at 30 µg

Predicted band size: 17 kDa

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Supplier Data

Western blot - Anti-LMO4 antibody (AB229226)

Samples were separated by 15% SDS-PAGE. The signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-LMO4 antibody (ab229226) at 1/1000 dilution

Lane 1:

T47D whole cell extracts at 30 µg

Lane 2:

MCF-7 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human LMO4.

P61968

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LIM-only protein 4 (LMO4) is a transcriptional regulator distinguished by its two LIM domains. It has an estimated molecular weight of approximately 22 kDa. LMO4 is expressed in several tissues but is notably present in the nervous system particularly the developing brain and spinal cord. The protein is also referred to as LMO4a in some contexts and plays a significant role in cellular processes by modulating gene expression.

Biological function summary

LMO4 participates in various developmental processes and acts as a cofactor within transcriptional complexes. It interacts with other proteins to regulate the transcription of genes critical for neural development and differentiation. LMO4 does not function in isolation but as part of larger multiprotein complexes influencing various signaling pathways linked to cell growth and differentiation.

Pathways

The LMO4 protein has strong connections to key signaling pathways including the Notch and Wnt pathways. These pathways are essential in neurogenesis and cellular differentiation processes. LMO4 interacts with proteins such as GATA3 and neurogenin acting as an intermediary that influences the transcriptional activity required for cellular development.

Scientists have linked alterations in LMO4 expression to conditions like breast cancer and neurodevelopmental disorders. Abnormal levels of LMO4 expression can disrupt cellular proliferation and differentiation contributing to pathogenesis. In breast cancer LMO4 interacts with the protein estrogen receptor promoting tumor growth and malignancy. Understanding these interactions helps unravel the molecular mechanisms underlying these diseases and offers targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription cofactor. Plays a role in establishing motor neuron identity, in concert with MNX1, acting, at least in part, to disrupt LDB1-LHX3 complexes thereby negatively modulating interneuron genes in motor neurons.

See full target information LMO4

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 23:77 PubMed39934774

2025

sdRNA-D43 derived from small nucleolar RNA snoRD43 improves chondrocyte senescence and osteoarthritis progression by negatively regulating PINK1/Parkin-mediated mitophagy pathway via dual-targeting NRF1 and WIPI2.

Applications

Unspecified application

Species

Unspecified reactive species

Zengfa Deng,Changzhao Li,Shu Hu,Yanlin Zhong,Wei Li,Zhencan Lin,Xiaolin Mo,Ming Li,Dongliang Xu,Dianbo Long,Guping Mao,Yan Kang

Neural plasticity 2022:3172861 PubMed35237315

2022

Preferential Involvement of BRCA1/BARD1, Not Tip60/Fe65, in DNA Double-Strand Break Repair in Presenilin-1 P117L Alzheimer Models.

Applications

Unspecified application

Species

Unspecified reactive species

Marcella M Authiat,Emmanuelle Gruz-Gibelli,Julien Colas,Estelle Bianchi,Marta Garcia-Arauzo,Pascale Marin,François R Herrmann,Armand Savioz

Frontiers in oncology 11:658712 PubMed34447693

2021

FOXM1 Promotes Head and Neck Squamous Cell Carcinoma Activation of the Linc-ROR/LMO4/AKT/PI3K Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Ma,Hong Zhang,Qian Li,Erik Schiferle,Yao Qin,Suifang Xiao,Tiancheng Li

Cancer management and research 12:9459-9470 PubMed33061633

2020

Circ_0058124 Aggravates the Progression of Papillary Thyroid Carcinoma by Activating LMO4 Expression via Targeting miR-370-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Liu,Chaohui Yan,Shudong Tao,Hailing Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LMO4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO4 antibody (AB229226)

Western blot - Anti-LMO4 antibody (AB229226)

Western blot - Anti-LMO4 antibody (AB229226)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise