AB131030

Anti-LMO4 antibody [EPR6731(2)]

RabMAb
Recombinant
What is this?

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(6 Publications)

Rabbit Recombinant Monoclonal LMO4 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 6 publications.

View Alternative Names

LIM domain transcription factor LMO4, Breast tumor autoantigen, LIM domain only protein 4, LMO-4, LMO4

5 Images

Lab

Immunoprecipitation - Anti-LMO4 antibody [EPR6731(2)] (AB131030)

Purified ab131030 at 1/20 immunoprecipitating LMO4 in Jurkat whole cell lysate observed at 18 KDa (lanes 1 and 2).

Lane 1 (input) : Jurkat whole cell lysate 10ug

Lane 2 (+) : ab131030 + Jurkat whole cell lysate.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab131030 in Jurkat whole cell lysate

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Blocking/Diluting buffer 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-LMO4 antibody [EPR6731(2)] (ab131030)

Predicted band size: 17 kDa

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Unknown

Western blot - Anti-LMO4 antibody [EPR6731(2)] (AB131030)

Actual band size : 17 kDa

All lanes:

Western blot - Anti-LMO4 antibody [EPR6731(2)] (ab131030) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Lane 4:

NIH3T3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 17 kDa

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Unknown

Western blot - Anti-LMO4 antibody [EPR6731(2)] (AB131030)

Blocking/Diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-LMO4 antibody [EPR6731(2)] (ab131030) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Jurkat (human acute T cell leukemia) whole cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Unknown

Western blot - Anti-LMO4 antibody [EPR6731(2)] (AB131030)

Blocking/Diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-LMO4 antibody [EPR6731(2)] (ab131030) at 1/1000 dilution

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 15 µg

Lane 2:

Mouse cerebral cortex tissue lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

OI-RD Scanning - Anti-LMO4 antibody [EPR6731(2)] (AB131030)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-LMO4 antibody [EPR6731(2)] (AB131030)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6731(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.13% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LIM-only protein 4 (LMO4) is a transcriptional regulator distinguished by its two LIM domains. It has an estimated molecular weight of approximately 22 kDa. LMO4 is expressed in several tissues but is notably present in the nervous system particularly the developing brain and spinal cord. The protein is also referred to as LMO4a in some contexts and plays a significant role in cellular processes by modulating gene expression.

Biological function summary

LMO4 participates in various developmental processes and acts as a cofactor within transcriptional complexes. It interacts with other proteins to regulate the transcription of genes critical for neural development and differentiation. LMO4 does not function in isolation but as part of larger multiprotein complexes influencing various signaling pathways linked to cell growth and differentiation.

Pathways

The LMO4 protein has strong connections to key signaling pathways including the Notch and Wnt pathways. These pathways are essential in neurogenesis and cellular differentiation processes. LMO4 interacts with proteins such as GATA3 and neurogenin acting as an intermediary that influences the transcriptional activity required for cellular development.

Scientists have linked alterations in LMO4 expression to conditions like breast cancer and neurodevelopmental disorders. Abnormal levels of LMO4 expression can disrupt cellular proliferation and differentiation contributing to pathogenesis. In breast cancer LMO4 interacts with the protein estrogen receptor promoting tumor growth and malignancy. Understanding these interactions helps unravel the molecular mechanisms underlying these diseases and offers targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription cofactor. Plays a role in establishing motor neuron identity, in concert with MNX1, acting, at least in part, to disrupt LDB1-LHX3 complexes thereby negatively modulating interneuron genes in motor neurons.

See full target information LMO4

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurobiology 62:10652-10669 PubMed40229456

2025

Remote Ischemic Postconditioning Improve Cerebral Ischemia-Reperfusion Injury Induced Cognitive Dysfunction through Suppressing Mitochondrial Apoptosis in Hippocampus via TK/BK/B2R-Mediated PI3K/AKT.

Applications

Unspecified application

Species

Unspecified reactive species

Haocheng Qin,Lu Su,Bao Zhou,Pengkun Yang,Yu-Lian Zhu,Dan Liang

Disease markers 2021:3977189 PubMed34925640

2021

CircLDLR Promotes Papillary Thyroid Carcinoma Tumorigenicity by Regulating miR-637/LMO4 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan-Ming Jiang,Wei Liu,Ling Jiang,Hongbin Chang

Mediators of inflammation 2021:1629783 PubMed34725544

2021

MicroRNA-139-5p Alleviates High Glucose-Triggered Human Retinal Pigment Epithelial Cell Injury by Targeting LIM-Only Factor 4.

Applications

Unspecified application

Species

Unspecified reactive species

Kan Shao,Gong Chen,Lili Xia,Cheng Chen,Shan Huang

Frontiers in oncology 11:658712 PubMed34447693

2021

FOXM1 Promotes Head and Neck Squamous Cell Carcinoma Activation of the Linc-ROR/LMO4/AKT/PI3K Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Ma,Hong Zhang,Qian Li,Erik Schiferle,Yao Qin,Suifang Xiao,Tiancheng Li

Oncology reports 43:1503-1515 PubMed32323846

2020

Long non‑coding RNA SNHG1 promotes breast cancer progression by regulation of LMO4.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Xiong,Yeqian Feng,Lun Li,Jia Yao,Meirong Zhou,Piao Zhao,Feilong Huang,Liyun Zeng,Liqin Yuan

Experimental cell research 381:105-111 PubMed31078568

2019

Inhibition of protein nitration prevents cisplatin-induced inactivation of STAT3 and promotes anti-apoptotic signaling in organ of Corti cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rita Rosati,Monazza Shahab,William L Neumann,Samson Jamesdaniel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LMO4 antibody [EPR6731(2)]

Immunoprecipitation - Anti-LMO4 antibody [EPR6731(2)] (AB131030)

Western blot - Anti-LMO4 antibody [EPR6731(2)] (AB131030)

Western blot - Anti-LMO4 antibody [EPR6731(2)] (AB131030)

Western blot - Anti-LMO4 antibody [EPR6731(2)] (AB131030)

OI-RD Scanning - Anti-LMO4 antibody [EPR6731(2)] (AB131030)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise