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Rabbit Recombinant Monoclonal LMO4 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications.

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Images

Western blot - Anti-LMO4 antibody [EPR6731(2)] (AB131030), expandable thumbnail
  • OI-RD Scanning - Anti-LMO4 antibody [EPR6731(2)] (AB131030), expandable thumbnail
  • Immunoprecipitation - Anti-LMO4 antibody [EPR6731(2)] (AB131030), expandable thumbnail
  • Western blot - Anti-LMO4 antibody [EPR6731(2)] (AB131030), expandable thumbnail
  • Western blot - Anti-LMO4 antibody [EPR6731(2)] (AB131030), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.13% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-P
Human
Not recommended
Tested
Tested
Not recommended
Mouse
Not recommended
Expected
Tested
Not recommended

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20 - 1/100
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Transcription cofactor. Plays a role in establishing motor neuron identity, in concert with MNX1, acting, at least in part, to disrupt LDB1-LHX3 complexes thereby negatively modulating interneuron genes in motor neurons.

Alternative names

LIM domain transcription factor LMO4, Breast tumor autoantigen, LIM domain only protein 4, LMO-4, LMO4

Recommended products

Rabbit Recombinant Monoclonal LMO4 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR6731(2)
Purification technique
Affinity purification Protein A
Dissociation constant
7.9 x 10-12 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The LIM-only protein 4 (LMO4) is a transcriptional regulator distinguished by its two LIM domains. It has an estimated molecular weight of approximately 22 kDa. LMO4 is expressed in several tissues but is notably present in the nervous system particularly the developing brain and spinal cord. The protein is also referred to as LMO4a in some contexts and plays a significant role in cellular processes by modulating gene expression.

Biological function summary

LMO4 participates in various developmental processes and acts as a cofactor within transcriptional complexes. It interacts with other proteins to regulate the transcription of genes critical for neural development and differentiation. LMO4 does not function in isolation but as part of larger multiprotein complexes influencing various signaling pathways linked to cell growth and differentiation.

Pathways

The LMO4 protein has strong connections to key signaling pathways including the Notch and Wnt pathways. These pathways are essential in neurogenesis and cellular differentiation processes. LMO4 interacts with proteins such as GATA3 and neurogenin acting as an intermediary that influences the transcriptional activity required for cellular development.

Associated diseases and disorders

Scientists have linked alterations in LMO4 expression to conditions like breast cancer and neurodevelopmental disorders. Abnormal levels of LMO4 expression can disrupt cellular proliferation and differentiation contributing to pathogenesis. In breast cancer LMO4 interacts with the protein estrogen receptor promoting tumor growth and malignancy. Understanding these interactions helps unravel the molecular mechanisms underlying these diseases and offers targets for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-LMO4 antibody [EPR6731(2)] (ab131030), expandable thumbnail

    Western blot - Anti-LMO4 antibody [EPR6731(2)] (ab131030)

    Actual band size : 17 kDa

    All lanes: Western blot - Anti-LMO4 antibody [EPR6731(2)] (ab131030) at 1/1000 dilution

    Lane 1: 293T cell lysate at 10 µg

    Lane 2: Jurkat cell lysate at 10 µg

    Lane 3: SH-SY5Y cell lysate at 10 µg

    Lane 4: NIH3T3 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 17 kDa

  • OI-RD Scanning - Anti-LMO4 antibody [EPR6731(2)] (ab131030), expandable thumbnail

    OI-RD Scanning - Anti-LMO4 antibody [EPR6731(2)] (ab131030)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Immunoprecipitation - Anti-LMO4 antibody [EPR6731(2)] (ab131030), expandable thumbnail

    Immunoprecipitation - Anti-LMO4 antibody [EPR6731(2)] (ab131030)

    Purified ab131030 at 1/20 immunoprecipitating LMO4 in Jurkat whole cell lysate observed at 18 KDa (lanes 1 and 2).

    Lane 1 (input): Jurkat whole cell lysate 10ug

    Lane 2 (+): ab131030 + Jurkat whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab131030 in Jurkat whole cell lysate

    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Blocking/Diluting buffer 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-LMO4 antibody [EPR6731(2)] (ab131030)

    Predicted band size: 17 kDa

  • Western blot - Anti-LMO4 antibody [EPR6731(2)] (ab131030), expandable thumbnail

    Western blot - Anti-LMO4 antibody [EPR6731(2)] (ab131030)

    Blocking/Diluting buffer 5% NFDM/TBST

    All lanes: Western blot - Anti-LMO4 antibody [EPR6731(2)] (ab131030) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney) whole cell lysate at 20 µg

    Lane 2: Jurkat (human acute T cell leukemia) whole cell lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 17 kDa

    Observed band size: 17 kDa

  • Western blot - Anti-LMO4 antibody [EPR6731(2)] (ab131030), expandable thumbnail

    Western blot - Anti-LMO4 antibody [EPR6731(2)] (ab131030)

    Blocking/Diluting buffer 5% NFDM/TBST

    All lanes: Western blot - Anti-LMO4 antibody [EPR6731(2)] (ab131030) at 1/1000 dilution

    Lane 1: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 15 µg

    Lane 2: Mouse cerebral cortex tissue lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 17 kDa

    Observed band size: 17 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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