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Rabbit Recombinant Monoclonal LMO4 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-P
Human
Not recommended
Tested
Tested
Not recommended
Mouse
Not recommended
Expected
Tested
Not recommended

Not recommended
Not recommended

Species

Mouse, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human

Dilution info

-

Notes

-

Target data

Function

Probable transcriptional factor.

Alternative names

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Rabbit Recombinant Monoclonal LMO4 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR6731(2)

Purification technique

Affinity purification Protein A

Dissociation constant

7.9 x 10-12 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab248352 is the carrier-free version of ab131030.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

Biological function summary

LMO4 participates in various developmental processes and acts as a cofactor within transcriptional complexes. It interacts with other proteins to regulate the transcription of genes critical for neural development and differentiation. LMO4 does not function in isolation but as part of larger multiprotein complexes influencing various signaling pathways linked to cell growth and differentiation.

Activity summary

The LIM-only protein 4 (LMO4) is a transcriptional regulator distinguished by its two LIM domains. It has an estimated molecular weight of approximately 22 kDa. LMO4 is expressed in several tissues but is notably present in the nervous system particularly the developing brain and spinal cord. The protein is also referred to as LMO4a in some contexts and plays a significant role in cellular processes by modulating gene expression.

Pathways

The LMO4 protein has strong connections to key signaling pathways including the Notch and Wnt pathways. These pathways are essential in neurogenesis and cellular differentiation processes. LMO4 interacts with proteins such as GATA3 and neurogenin acting as an intermediary that influences the transcriptional activity required for cellular development.

Associated diseases and disorders

Scientists have linked alterations in LMO4 expression to conditions like breast cancer and neurodevelopmental disorders. Abnormal levels of LMO4 expression can disrupt cellular proliferation and differentiation contributing to pathogenesis. In breast cancer LMO4 interacts with the protein estrogen receptor promoting tumor growth and malignancy. Understanding these interactions helps unravel the molecular mechanisms underlying these diseases and offers targets for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352), expandable thumbnail

    Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)

    This data was developed using ab131030, the same antibody clone in a different buffer formulation.

    Actual band size : 17 kDa.

    All lanes: Western blot - Anti-LMO4 antibody [EPR6731(2)] (AB131030) at 1/1000 dilution

    Lane 1: 293T cell lysate at 10 µg

    Lane 2: Jurkat cell lysate at 10 µg

    Lane 3: SH-SY5Y cell lysate at 10 µg

    Lane 4: NIH3T3 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 17 kDa

  • OI-RD Scanning - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352), expandable thumbnail

    OI-RD Scanning - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Immunoprecipitation - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352), expandable thumbnail

    Immunoprecipitation - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)

    This data was developed using ab131030, the same antibody clone in a different buffer formulation.Purified ab131030 at 1/20 immunoprecipitating LMO4 in Jurkat whole cell lysate observed at 18 KDa (lanes 1 and 2).

    Lane 1 (input): Jurkat whole cell lysate 10ug

    Lane 2 (+): ab131030 + Jurkat whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab131030 in Jurkat whole cell lysate

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Blocking/Diluting buffer 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-LMO4 antibody [EPR6731(2)] (AB131030)

    Predicted band size: 17 kDa

  • Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352), expandable thumbnail

    Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)

    This data was developed using ab131030, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-LMO4 antibody [EPR6731(2)] (AB131030) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney) whole cell lysate at 20 µg

    Lane 2: Jurkat (human acute T cell leukemia) whole cell lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 17 kDa

    Observed band size: 17 kDa

  • Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352), expandable thumbnail

    Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)

    This data was developed using ab131030, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-LMO4 antibody [EPR6731(2)] (AB131030) at 1/1000 dilution

    Lane 1: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 15 µg

    Lane 2: Mouse cerebral cortex tissue lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 17 kDa

    Observed band size: 17 kDa

    Blocking/Diluting buffer 5% NFDM/TBST

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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