Rabbit Recombinant Monoclonal LMO4 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse samples.
Images
![Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (AB248352), expandable thumbnail](https://content.abcam.com/products/images/lmo4-antibody-epr67312-bsa-and-azide-free-ab248352--western-blot-img111679.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (AB248352)")
![OI-RD Scanning - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (AB248352), expandable thumbnail](https://content.abcam.com/products/images/lmo4-antibody-epr67312-bsa-and-azide-free-ab248352--oi-rd-scanning-img138241.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (AB248352)")
![Immunoprecipitation - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (AB248352), expandable thumbnail](https://content.abcam.com/products/images/lmo4-antibody-epr67312-bsa-and-azide-free-ab248352--immunoprecipitation-img20197.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (AB248352)")
![Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (AB248352), expandable thumbnail](https://content.abcam.com/products/images/lmo4-antibody-epr67312-bsa-and-azide-free-ab248352--western-blot-img87385.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (AB248352)")
![Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (AB248352), expandable thumbnail](https://content.abcam.com/products/images/lmo4-antibody-epr67312-bsa-and-azide-free-ab248352--western-blot-img105689.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (AB248352)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Not recommended |
| Mouse | Not recommended | Expected | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Target data
Function
Transcription cofactor. Plays a role in establishing motor neuron identity, in concert with MNX1, acting, at least in part, to disrupt LDB1-LHX3 complexes thereby negatively modulating interneuron genes in motor neurons.
Alternative names
LIM domain transcription factor LMO4, Breast tumor autoantigen, LIM domain only protein 4, LMO-4, LMO4
Recommended products
Rabbit Recombinant Monoclonal LMO4 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6731(2)
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 7.9 x 10-12 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248352 is the carrier-free version of Anti-LMO4 antibody [EPR6731(2)] ab131030.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The LIM-only protein 4 (LMO4) is a transcriptional regulator distinguished by its two LIM domains. It has an estimated molecular weight of approximately 22 kDa. LMO4 is expressed in several tissues but is notably present in the nervous system particularly the developing brain and spinal cord. The protein is also referred to as LMO4a in some contexts and plays a significant role in cellular processes by modulating gene expression.
- Biological function summary
LMO4 participates in various developmental processes and acts as a cofactor within transcriptional complexes. It interacts with other proteins to regulate the transcription of genes critical for neural development and differentiation. LMO4 does not function in isolation but as part of larger multiprotein complexes influencing various signaling pathways linked to cell growth and differentiation.
- Pathways
The LMO4 protein has strong connections to key signaling pathways including the Notch and Wnt pathways. These pathways are essential in neurogenesis and cellular differentiation processes. LMO4 interacts with proteins such as GATA3 and neurogenin acting as an intermediary that influences the transcriptional activity required for cellular development.
- Associated diseases and disorders
Scientists have linked alterations in LMO4 expression to conditions like breast cancer and neurodevelopmental disorders. Abnormal levels of LMO4 expression can disrupt cellular proliferation and differentiation contributing to pathogenesis. In breast cancer LMO4 interacts with the protein estrogen receptor promoting tumor growth and malignancy. Understanding these interactions helps unravel the molecular mechanisms underlying these diseases and offers targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352), expandable thumbnail](https://content.abcam.com/products/images/lmo4-antibody-epr67312-bsa-and-azide-free-ab248352--western-blot-img111679.jpg "Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)")
Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)
This data was developed using Anti-LMO4 antibody [EPR6731(2)] ab131030, the same antibody clone in a different buffer formulation.
Actual band size : 17 kDa.
All lanes: Western blot - Anti-LMO4 antibody [EPR6731(2)] (Anti-LMO4 antibody [EPR6731(2)] ab131030) at 1/1000 dilution
Lane 1: 293T cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: SH-SY5Y cell lysate at 10 µg
Lane 4: NIH3T3 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 17 kDa
![OI-RD Scanning - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352), expandable thumbnail](https://content.abcam.com/products/images/lmo4-antibody-epr67312-bsa-and-azide-free-ab248352--oi-rd-scanning-img138241.jpg "OI-RD Scanning - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)")
OI-RD Scanning - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.![Immunoprecipitation - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352), expandable thumbnail](https://content.abcam.com/products/images/lmo4-antibody-epr67312-bsa-and-azide-free-ab248352--immunoprecipitation-img20197.jpg "Immunoprecipitation - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)")
Immunoprecipitation - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)
This data was developed using Anti-LMO4 antibody [EPR6731(2)] ab131030, the same antibody clone in a different buffer formulation.Purified Anti-LMO4 antibody [EPR6731(2)] ab131030 at 1/20 immunoprecipitating LMO4 in Jurkat whole cell lysate observed at 18 KDa (lanes 1 and 2). Lane 1 (input): Jurkat whole cell lysate 10ug Lane 2 (+): Anti-LMO4 antibody [EPR6731(2)] ab131030 + Jurkat whole cell lysate. Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-LMO4 antibody [EPR6731(2)] ab131030 in Jurkat whole cell lysate VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution. Blocking/Diluting buffer 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-LMO4 antibody [EPR6731(2)] (Anti-LMO4 antibody [EPR6731(2)] ab131030)
Predicted band size: 17 kDa
![Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352), expandable thumbnail](https://content.abcam.com/products/images/lmo4-antibody-epr67312-bsa-and-azide-free-ab248352--western-blot-img87385.jpg "Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)")
Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)
This data was developed using Anti-LMO4 antibody [EPR6731(2)] ab131030, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-LMO4 antibody [EPR6731(2)] (Anti-LMO4 antibody [EPR6731(2)] ab131030) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney) whole cell lysate at 20 µg
Lane 2: Jurkat (human acute T cell leukemia) whole cell lysate at 20 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
![Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352), expandable thumbnail](https://content.abcam.com/products/images/lmo4-antibody-epr67312-bsa-and-azide-free-ab248352--western-blot-img105689.jpg "Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)")
Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)
This data was developed using Anti-LMO4 antibody [EPR6731(2)] ab131030, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-LMO4 antibody [EPR6731(2)] (Anti-LMO4 antibody [EPR6731(2)] ab131030) at 1/1000 dilution
Lane 1: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 15 µg
Lane 2: Mouse cerebral cortex tissue lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
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