Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free

• IP

Lab

Immunoprecipitation - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (AB248352)

This data was developed using ab131030, the same antibody clone in a different buffer formulation.

Purified ab131030 at 1/20 immunoprecipitating LMO4 in Jurkat whole cell lysate observed at 18 KDa (lanes 1 and 2).

Lane 1 (input) : Jurkat whole cell lysate 10ug

Lane 2 (+) : ab131030 + Jurkat whole cell lysate.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab131030 in Jurkat whole cell lysate

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Blocking/Diluting buffer 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-LMO4 antibody [EPR6731(2)] (<a href='/en-us/products/primary-antibodies/lmo4-antibody-epr67312-ab131030'>ab131030</a>)

Predicted band size: 17 kDa

false

• WB

Unknown

Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (AB248352)

This data was developed using ab131030, the same antibody clone in a different buffer formulation.

Actual band size : 17 kDa.

All lanes:

Western blot - Anti-LMO4 antibody [EPR6731(2)] (<a href='/en-us/products/primary-antibodies/lmo4-antibody-epr67312-ab131030'>ab131030</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Lane 4:

NIH3T3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 17 kDa

false

• WB

Unknown

Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (AB248352)

This data was developed using ab131030, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-LMO4 antibody [EPR6731(2)] (<a href='/en-us/products/primary-antibodies/lmo4-antibody-epr67312-ab131030'>ab131030</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Jurkat (human acute T cell leukemia) whole cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

• WB

Unknown

Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (AB248352)

This data was developed using ab131030, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-LMO4 antibody [EPR6731(2)] (<a href='/en-us/products/primary-antibodies/lmo4-antibody-epr67312-ab131030'>ab131030</a>) at 1/1000 dilution

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 15 µg

Lane 2:

Mouse cerebral cortex tissue lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (AB248352)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.