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AB224113

Anti-LMO7 antibody

5

(1 Review)

|

(4 Publications)

Rabbit Polyclonal LMO7 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LMO7 aa 650-850.

View Alternative Names

FBX20, FBXO20, KIAA0858, LMO7, LIM domain only protein 7, LMO-7, F-box only protein 20, LOMP

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO7 antibody (AB224113)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO7 antibody (AB224113)

Paraffin-embedded human skeletal muscle tissue stained for LMO7 using ab224113 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO7 antibody (AB224113)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO7 antibody (AB224113)

Paraffin-embedded human testis tissue stained for LMO7 using ab224113 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO7 antibody (AB224113)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO7 antibody (AB224113)

Paraffin-embedded human uterine cervix tissue stained for LMO7 using ab224113 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-LMO7 antibody (AB224113)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LMO7 antibody (AB224113)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for LMO7 (green) using ab224113 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO7 antibody (AB224113)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO7 antibody (AB224113)

Paraffin-embedded human duodenum tissue stained for LMO7 using ab224113 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-LMO7 antibody (AB224113)
  • WB

Supplier Data

Western blot - Anti-LMO7 antibody (AB224113)

Western blot analysis of HeLa cell lysate using ab224113 at 0.4 μg/ml.

All lanes:

Western blot - Anti-LMO7 antibody (ab224113) at 0.4 µg/mL

Predicted band size: 193 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human LMO7 aa 650-850. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LMO7 known also as LIM-only protein 7 plays a significant mechanical role in cellular architecture and gene expression. This protein with a molecular mass of approximately 165 kDa not only localizes to the nuclear envelope but also to the cytoplasm. It is found in many tissues with higher expression levels noted in skeletal muscle and heart tissue. LMO7 contains LIM domains that facilitate protein-protein interactions important for its varied functions within the cell.
Biological function summary

LMO7 supports cellular integrity and function through its roles at the nuclear periphery. It interacts with nuclear and cytoskeletal elements contributing to processes such as nuclear shaping and stability. LMO7 participates in the assembly of the mechanotransduction complex at the nuclear membrane. This role links external mechanical signals to gene expression regulation involving other proteins in the complex and helping cells respond to mechanical stress.

Pathways

This protein integrates into the mechanotransduction pathway and the actin cytoskeleton signaling. It interacts with cellular components such as emerin and nesprin impacting cytoskeletal dynamics and nuclear positioning. In doing so LMO7 influences signal transduction pathways that affect cell migration and structural maintenance which represent important physiological functions.

LMO7 associations appear in certain muscular dystrophies and cancer. Its interaction with proteins like emerin suggests implications in Emery-Dreifuss muscular dystrophy a condition characterized by weakened skeletal muscles. Additionally altered LMO7 expression or function has connections to certain cancers where it may affect tumor suppressor pathways and metastatic potential linking it indirectly to aberrations in cell adhesion and motility.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information LMO7
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106600 PubMed37213233

2023

FAP promotes metastasis and chemoresistance via regulating YAP1 and macrophages in mucinous colorectal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dengbo Ji,Jinying Jia,Xinxin Cui,Zhaowei Li,Aiwen Wu

Stem cells international 2023:2826815 PubMed37964983

2023

A Novel Molecular Classification Method for Glioblastoma Based on Tumor Cell Differentiation Trajectories.

Applications

Unspecified application

Species

Unspecified reactive species

Guanghao Zhang,Xiaolong Xu,Luojiang Zhu,Sisi Li,Rundong Chen,Nan Lv,Zifu Li,Jing Wang,Qiang Li,Wang Zhou,Pengfei Yang,Jianmin Liu

Bone research 11:1 PubMed36588108

2023

A novel molecular classification method for osteosarcoma based on tumor cell differentiation trajectories.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhang,Ting Wang,Haiyi Gong,Runyi Jiang,Wang Zhou,Haitao Sun,Runzhi Huang,Yao Wang,Zhipeng Wu,Wei Xu,Zhenxi Li,Quan Huang,Xiaopan Cai,Zaijun Lin,Jinbo Hu,Qi Jia,Chen Ye,Haifeng Wei,Jianru Xiao

JOR spine 5:e1210 PubMed36601375

2022

Comparative proteome analysis of the ligamentum flavum of patients with lumbar spinal canal stenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yutaka Yabe,Yoshihiro Hagiwara,Masahiro Tsuchiya,Takashi Minowa,Taro Takemura,Shinya Hattori,Shinichirou Yoshida,Takahiro Onoki,Keisuke Ishikawa
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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