Rabbit Polyclonal LMO7 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LIM domain only protein 7 aa 650-850.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Select an associated product type
FBX20, FBXO20, KIAA0858, FBX20, KIAA0858, FBXO20, LMO7, LIM domain only protein 7, LMO-7, F-box only protein 20, LOMP
Rabbit Polyclonal LMO7 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LIM domain only protein 7 aa 650-850.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This supplementary information is collated from multiple sources and compiled automatically.
LMO7 known also as LIM-only protein 7 plays a significant mechanical role in cellular architecture and gene expression. This protein with a molecular mass of approximately 165 kDa not only localizes to the nuclear envelope but also to the cytoplasm. It is found in many tissues with higher expression levels noted in skeletal muscle and heart tissue. LMO7 contains LIM domains that facilitate protein-protein interactions important for its varied functions within the cell.
LMO7 supports cellular integrity and function through its roles at the nuclear periphery. It interacts with nuclear and cytoskeletal elements contributing to processes such as nuclear shaping and stability. LMO7 participates in the assembly of the mechanotransduction complex at the nuclear membrane. This role links external mechanical signals to gene expression regulation involving other proteins in the complex and helping cells respond to mechanical stress.
This protein integrates into the mechanotransduction pathway and the actin cytoskeleton signaling. It interacts with cellular components such as emerin and nesprin impacting cytoskeletal dynamics and nuclear positioning. In doing so LMO7 influences signal transduction pathways that affect cell migration and structural maintenance which represent important physiological functions.
LMO7 associations appear in certain muscular dystrophies and cancer. Its interaction with proteins like emerin suggests implications in Emery-Dreifuss muscular dystrophy a condition characterized by weakened skeletal muscles. Additionally altered LMO7 expression or function has connections to certain cancers where it may affect tumor suppressor pathways and metastatic potential linking it indirectly to aberrations in cell adhesion and motility.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for LMO7 (green) using ab224113 at 4 μg/ml in ICC/IF.
Western blot analysis of HeLa cell lysate using ab224113 at 0.4 μg/ml.
All lanes: Western blot - Anti-LMO7 antibody (ab224113) at 0.4 µg/mL
Predicted band size: 193 kDa
Paraffin-embedded human duodenum tissue stained for LMO7 using ab224113 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human skeletal muscle tissue stained for LMO7 using ab224113 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human testis tissue stained for LMO7 using ab224113 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human uterine cervix tissue stained for LMO7 using ab224113 at 1/200 dilution in immunohistochemical analysis.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com