Rabbit Polyclonal Kininogen antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human KNG1.
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Publications
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- RNA (New York, N.Y.) 25:255-2632018An investigational RNAi therapeutic targeting Factor XII (ALN-F12) for the treatment of hereditary angioedema.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingxuan Liu,June Qin,Anna Borodovsky,Timothy Racie,Adam Castoreno,Mark Schlegel,Martin A Maier,Tracy Zimmerman,Kevin Fitzgerald,James Butler,Akin AkincPubMed 30463937
- Cancer epidemiology, biomarkers & prevention : a p 26:795-8032017Changes in the Glycosylation of Kininogen and the Development of a Kininogen-Based Algorithm for the Early Detection of HCC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengjun Wang et. al.PubMed 28223431
- Journal of proteomics 91:478-852013Proteomic analysis of B-cell receptor signaling in chronic lymphocytic leukaemia reveals a possible role for kininogen.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElena Kashuba et. al.PubMed 23938224
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.98% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Native Full Length Protein corresponding to Human KNG1. Database link P01042
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 20 µg/mL | Notes - |
Target data
Function
Kininogens are inhibitors of thiol proteases. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. LMW-kininogen inhibits the aggregation of thrombocytes. LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting. Bradykinin. The active peptide bradykinin is a potent vasodilatator that is released from HMW-kininogen shows a variety of physiological effects: (A) influence in smooth muscle contraction, (B) induction of hypotension, (C) natriuresis and diuresis, (D) decrease in blood glucose level, (E) it is a mediator of inflammation and causes (E1) increase in vascular permeability, (E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action).
Alternative names
BDK, KNG, KNG1, Kininogen-1, Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, High molecular weight kininogen, Williams-Fitzgerald-Flaujeac factor, HMWK
Recommended products
Rabbit Polyclonal Kininogen antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human KNG1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.98% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Native Full Length Protein corresponding to Human KNG1. Database link P01042
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LMW Kininogen also known as Low Molecular Weight Kininogen has a molecular mass of approximately 68 kDa. This protein is produced mainly in the liver and circulates in the plasma. It serves as a precursor for the vasoactive peptide bradykinin and plays an important role in the kallikrein-kinin system. LMW Kininogen cleaves to form bradykinin upon activation which is mediated by tissue kallikrein and plasma kallikrein. Additionally LMW Kininogen interacts with high molecular weight kininogen sharing some similar functions in the body.
- Biological function summary
LMW Kininogen contributes to processes like inflammation and blood pressure regulation by releasing bradykinin. This protein does not act alone; it is integral to a larger complex involving kallikreins and kininogens. Bradykinin generated from LMW Kininogen increases vascular permeability and causes smooth muscle contractions. This ability to form active peptides makes LMW Kininogen significant in vascular homeostasis and in controlling inflammatory responses.
- Pathways
LMW Kininogen participates in the kallikrein-kinin and coagulation pathways. In the kallikrein-kinin pathway LMW Kininogen acts as a substrate leading to the generation of bradykinin and other kinins. It also influences the coagulation pathway by modulating the activity of intrinsic coagulation enzymes. This protein has connections with other pathway elements like high molecular weight kininogen and factor XII highlighting its role in inflammation and coagulation regulation.
- Associated diseases and disorders
LMW Kininogen relates to conditions like hereditary angioedema and inflammatory diseases. Hereditary angioedema often involves a deficiency or dysfunction in the enzymes that interact with LMW Kininogen leading to excessive bradykinin production and swelling. Inflammatory disorders may also connect to LMW Kininogen through its regulation of blood pressure and inflammation via the bradykinin pathway. Examining the links with other proteins such as factor XII and high molecular weight kininogen can provide further insights into these disease processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-LMW Kininogen antibody (ab79650)
All lanes: Western blot - Anti-LMW Kininogen antibody (ab79650) at 1 µg/mL
All lanes: Western blot - Native Human LMW Kininogen protein (Native Human LMW Kininogen protein ab91118) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Exposure time: 10s
This image is courtesy of an anonymous customer reviewWestern blot - Anti-LMW Kininogen antibody (ab79650)
Blocked with 5% non-fat milk for 15 hours at 4°C
Lane 1: Western blot - Anti-LMW Kininogen antibody (ab79650) at 1/100 dilution
Lane 2: Coomassie Blue staining
All lanes: Western blot - Native Human LMW Kininogen protein (Native Human LMW Kininogen protein ab91118) at 0.01 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit monoclonal at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 72 kDa
Exposure time: 20min
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