AB79650

Anti-LMW Kininogen antibody

Name: Anti-LMW Kininogen antibody (ab79650) | Abcam
Brand: Abcam Limited
SKU: AB79650
Rating: 4 (2 reviews)

(2 Reviews)

(3 Publications)

Rabbit Polyclonal Kininogen antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human KNG1.

View Alternative Names

BDK, KNG, KNG1, Kininogen-1, Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, High molecular weight kininogen, Williams-Fitzgerald-Flaujeac factor, HMWK

2 Images

AbReview30413****

Western blot - Anti-LMW Kininogen antibody (AB79650)

Blocked with 5% non-fat milk for 15 hours at 4°C

Lane 1:

Western blot - Anti-LMW Kininogen antibody (ab79650) at 1/100 dilution

Lane 2:

Coomassie Blue staining

All lanes:

Western blot - Native Human LMW Kininogen protein (<a href='/en-us/products/proteins-peptides/native-human-lmw-kininogen-protein-ab91118'>ab91118</a>) at 0.01 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit monoclonal at 1/1000 dilution

Predicted band size: 72 kDa

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Exposure time: 20min

This image is courtesy of an anonymous Abreview

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Western blot - Anti-LMW Kininogen antibody (AB79650)

All lanes:

Western blot - Anti-LMW Kininogen antibody (ab79650) at 1 µg/mL

All lanes:

Western blot - Native Human LMW Kininogen protein (<a href='/en-us/products/proteins-peptides/native-human-lmw-kininogen-protein-ab91118'>ab91118</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 72 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Native Full Length Protein corresponding to Human KNG1.

P01042

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "20 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LMW Kininogen also known as Low Molecular Weight Kininogen has a molecular mass of approximately 68 kDa. This protein is produced mainly in the liver and circulates in the plasma. It serves as a precursor for the vasoactive peptide bradykinin and plays an important role in the kallikrein-kinin system. LMW Kininogen cleaves to form bradykinin upon activation which is mediated by tissue kallikrein and plasma kallikrein. Additionally LMW Kininogen interacts with high molecular weight kininogen sharing some similar functions in the body.

Biological function summary

LMW Kininogen contributes to processes like inflammation and blood pressure regulation by releasing bradykinin. This protein does not act alone; it is integral to a larger complex involving kallikreins and kininogens. Bradykinin generated from LMW Kininogen increases vascular permeability and causes smooth muscle contractions. This ability to form active peptides makes LMW Kininogen significant in vascular homeostasis and in controlling inflammatory responses.

Pathways

LMW Kininogen participates in the kallikrein-kinin and coagulation pathways. In the kallikrein-kinin pathway LMW Kininogen acts as a substrate leading to the generation of bradykinin and other kinins. It also influences the coagulation pathway by modulating the activity of intrinsic coagulation enzymes. This protein has connections with other pathway elements like high molecular weight kininogen and factor XII highlighting its role in inflammation and coagulation regulation.

LMW Kininogen relates to conditions like hereditary angioedema and inflammatory diseases. Hereditary angioedema often involves a deficiency or dysfunction in the enzymes that interact with LMW Kininogen leading to excessive bradykinin production and swelling. Inflammatory disorders may also connect to LMW Kininogen through its regulation of blood pressure and inflammation via the bradykinin pathway. Examining the links with other proteins such as factor XII and high molecular weight kininogen can provide further insights into these disease processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Kininogens are inhibitors of thiol proteases. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. LMW-kininogen inhibits the aggregation of thrombocytes. LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.. Bradykinin. The active peptide bradykinin is a potent vasodilatator that is released from HMW-kininogen shows a variety of physiological effects : (A) influence in smooth muscle contraction, (B) induction of hypotension, (C) natriuresis and diuresis, (D) decrease in blood glucose level, (E) it is a mediator of inflammation and causes (E1) increase in vascular permeability, (E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action).

See full target information KNG1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

RNA (New York, N.Y.) 25:255-263 PubMed30463937

2018

An investigational RNAi therapeutic targeting Factor XII (ALN-F12) for the treatment of hereditary angioedema.

Applications

Unspecified application

Species

Unspecified reactive species

Jingxuan Liu,June Qin,Anna Borodovsky,Timothy Racie,Adam Castoreno,Mark Schlegel,Martin A Maier,Tracy Zimmerman,Kevin Fitzgerald,James Butler,Akin Akinc

Cancer epidemiology, biomarkers & prevention : a p 26:795-803 PubMed28223431

2017

Changes in the Glycosylation of Kininogen and the Development of a Kininogen-Based Algorithm for the Early Detection of HCC.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjun Wang,Miloslav Sanda,Mary Ann Comunale,Harmin Herrera,Charles Swindell,Yuko Kono,Amit G Singal,Jorge Marrero,Timothy Block,Radoslav Goldman,Anand Mehta

Journal of proteomics 91:478-85 PubMed23938224

2013

Proteomic analysis of B-cell receptor signaling in chronic lymphocytic leukaemia reveals a possible role for kininogen.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Kashuba,Gina L Eagle,James Bailey,Paul Evans,Kevin J Welham,David Allsup,Lynn Cawkwell

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LMW Kininogen antibody

Western blot - Anti-LMW Kininogen antibody (AB79650)

Western blot - Anti-LMW Kininogen antibody (AB79650)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise