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Rabbit Recombinant Monoclonal LMX1A antibody. Carrier free. Suitable for WB, IHC-P and reacts with Recombinant fragment - Human, Mouse, Rat samples.

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Images

Western blot - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (AB309539), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (AB309539), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (AB309539), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (AB309539), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (AB309539), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)IP
Human
Not recommended
Predicted
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species

Rat

Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat, Recombinant fragment - Human

Dilution info

-

Notes

-

Target data

Function

Acts as a transcriptional activator by binding to an A/T-rich sequence, the FLAT element, in the insulin gene promoter. Required for development of the roof plate and, in turn, for specification of dorsal cell fates in the CNS and developing vertebrae (By similarity).

Alternative names

LIM homeobox transcription factor 1-alpha, LIM/homeobox protein 1.1, LIM/homeobox protein LMX1A, LMX-1.1, LMX1A

Recommended products

Rabbit Recombinant Monoclonal LMX1A antibody. Carrier free. Suitable for WB, IHC-P and reacts with Recombinant fragment - Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR27269-57

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

LMX1A also known as LIM homeobox transcription factor 1 alpha serves as a transcription factor involved in embryonic development. It belongs to the LIM homeobox gene family and weighs approximately 42 kDa. This protein primarily localizes in the central nervous system and developing limb tissues. It plays an important role in the differentiation and development of dopamine-producing neurons which are important for proper nervous system function.

Biological function summary

The presence of LMX1A is essential for the development of dopaminergic neurons in the midbrain. It functions as part of a complex transcriptional network with other key transcription factors such as NURR1 and PITX3 to regulate genes essential for dopamine synthesis and neuron maintenance. Through its actions LMX1A influences the dopaminergic system contributing to motor control and behavior regulation. This makes it important for studies in neurobiology and developmental biology.

Pathways

LMX1A is an integral component in the Wnt signaling pathway which is vital for maintaining dopaminergic neuron populations. It directly impacts the regulation and expression of genes related to dopamine synthesis. In collaboration with transcription factors like NURR1 LMX1A promotes the production of enzymes necessary for dopamine metabolism. This pathway plays an important role in neuronal survival and connectivity illustrating the connection of LMX1A to other proteins within this system.

Associated diseases and disorders

Alterations in LMX1A expression or function are linked to Parkinson's disease and neural developmental disorders. In Parkinson's disease the degeneration of dopaminergic neurons occurs where LMX1A plays an important role in neuronal development and maintenance showcasing its potential therapeutic significance. Furthermore downregulation of LMX1A can lead to a disrupted neuronal development illustrating its impact on diseases associated with neurological development while the protein NURR1 is also critically involved in these processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (ab309539), expandable thumbnail

    Western blot - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (ab309539)

    This data was developed using Anti-LMX1A antibody [EPR27269-57] ab309538, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This antibody does not cross-react with human LMX1B.
    In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
    Exposure time: 15 seconds

    All lanes: Western blot - Anti-LMX1A antibody [EPR27269-57] (Anti-LMX1A antibody [EPR27269-57] ab309538) at 1/1000 dilution

    Lane 1: His-tagged human LMX1B recombinant protein at 20 µg

    Lane 2: His-tagged human LMX1A recombinant protein at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 42 kDa

    Exposure time: 15s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This antibody does not cross-react with human LMX1B.
    In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
    Exposure time: 15 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (ab309539), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (ab309539)

    This data was developed using Anti-LMX1A antibody [EPR27269-57] ab309538, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling LMX1A with Anti-LMX1A antibody [EPR27269-57] ab309538 at 1/5000 (0.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on rat liver. The section was incubated with Anti-LMX1A antibody [EPR27269-57] ab309538 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (ab309539), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (ab309539)

    This data was developed using Anti-LMX1A antibody [EPR27269-57] ab309538, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling LMX1A with Anti-LMX1A antibody [EPR27269-57] ab309538 at 1/500 (1.002 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on mouse liver. The section was incubated with Anti-LMX1A antibody [EPR27269-57] ab309538 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (ab309539), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (ab309539)

    This data was developed using Anti-LMX1A antibody [EPR27269-57] ab309538, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Rat E12.5 brain tissue labeling LMX1A with Anti-LMX1A antibody [EPR27269-57] ab309538 at 1/5000 (0.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat E12.5 brain. The section was incubated with Anti-LMX1A antibody [EPR27269-57] ab309538 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (ab309539), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (ab309539)

    This data was developed using Anti-LMX1A antibody [EPR27269-57] ab309538, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Mouse E12.5 brain tissue labeling LMX1A with Anti-LMX1A antibody [EPR27269-57] ab309538 at 1/500 (1.002 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse E12.5 brain (PMID: 16439212). The section was incubated with Anti-LMX1A antibody [EPR27269-57] ab309538 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (ab309539), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (ab309539)

    This data was developed using Anti-LMX1A antibody [EPR27269-57] ab309538, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling LMX1A with Anti-LMX1A antibody [EPR27269-57] ab309538 at 1/500 (1.002 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on ventral midbrain of mouse (PMID: 27407143). The section was incubated with Anti-LMX1A antibody [EPR27269-57] ab309538 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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