Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (AB309539)

This data was developed using ab309538, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling LMX1A with ab309538 at 1/5000 (0.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on rat liver. The section was incubated with ab309538 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (AB309539)

This data was developed using ab309538, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling LMX1A with ab309538 at 1/500 (1.002 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse liver. The section was incubated with ab309538 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (AB309539)

This data was developed using ab309538, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse E12.5 brain tissue labeling LMX1A with ab309538 at 1/500 (1.002 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse E12.5 brain (PMID : 16439212). The section was incubated with ab309538 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (AB309539)

This data was developed using ab309538, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat E12.5 brain tissue labeling LMX1A with ab309538 at 1/5000 (0.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat E12.5 brain. The section was incubated with ab309538 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (AB309539)

This data was developed using ab309538, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling LMX1A with ab309538 at 1/500 (1.002 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on ventral midbrain of mouse (PMID : 27407143). The section was incubated with ab309538 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-LMX1A antibody [EPR27269-57] - BSA and Azide free (AB309539)

This data was developed using ab309538, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human LMX1B. In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. Exposure time : 15 seconds

All lanes:

Western blot - Anti-LMX1A antibody [EPR27269-57] (<a href='/en-us/products/primary-antibodies/lmx1a-antibody-epr27269-57-ab309538'>ab309538</a>) at 1/1000 dilution

Lane 1:

His-tagged human LMX1B recombinant protein at 20 µg

Lane 2:

His-tagged human LMX1A recombinant protein at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 42 kDa

false

Exposure time: 15s