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AB308029

Anti-LMX1A antibody [RP23040133]

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Rabbit Recombinant Multiclonal LMX1A antibody. Suitable for WB and reacts with Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human LMX1A.

View Alternative Names

LIM homeobox transcription factor 1-alpha, LIM/homeobox protein 1.1, LIM/homeobox protein LMX1A, LMX-1.1, LMX1A

1 Images
Western blot - Anti-LMX1A antibody [RP23040133] (AB308029)
  • WB

Supplier Data

Western blot - Anti-LMX1A antibody [RP23040133] (AB308029)

Western blot analysis was performed on tissue extracts (30 µg lysate) of Rat Brain (Lane 1), Mouse Brain (Lane 2), Mouse Cerebellum (Lane 3) and Mouse heart (Lane 4). The blots were probed withab308029, 1-2 µg/mL and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (0.4 µg/mL, 1 : 2500 dilution). A 42 kDa band corresponding to Lmx1a was observed according to the tested tissues. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane.. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).

All lanes:

Western blot - Anti-LMX1A antibody [RP23040133] (ab308029) at 1 µg/mL

Lane 1:

Rat brain tissue extracts at 30 µg

Lane 2:

Mouse Brain tissue extracts at 30 µg

Lane 3:

Mouse Cerebellum tissue extracts at 30 µg

Lane 4:

Mouse Heart tissue extracts at 30 µg

Secondary

All lanes:

HRP-conjugate Goat anti-Rabbit IgG (H+L) Secondary Antibody at 0.4 µg/mL

Predicted band size: 102 kDa

Observed band size: 102 kDa

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Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040133

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human LMX1A.

Q8TE12

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LMX1A also known as LIM homeobox transcription factor 1 alpha serves as a transcription factor involved in embryonic development. It belongs to the LIM homeobox gene family and weighs approximately 42 kDa. This protein primarily localizes in the central nervous system and developing limb tissues. It plays an important role in the differentiation and development of dopamine-producing neurons which are important for proper nervous system function.
Biological function summary

The presence of LMX1A is essential for the development of dopaminergic neurons in the midbrain. It functions as part of a complex transcriptional network with other key transcription factors such as NURR1 and PITX3 to regulate genes essential for dopamine synthesis and neuron maintenance. Through its actions LMX1A influences the dopaminergic system contributing to motor control and behavior regulation. This makes it important for studies in neurobiology and developmental biology.

Pathways

LMX1A is an integral component in the Wnt signaling pathway which is vital for maintaining dopaminergic neuron populations. It directly impacts the regulation and expression of genes related to dopamine synthesis. In collaboration with transcription factors like NURR1 LMX1A promotes the production of enzymes necessary for dopamine metabolism. This pathway plays an important role in neuronal survival and connectivity illustrating the connection of LMX1A to other proteins within this system.

Alterations in LMX1A expression or function are linked to Parkinson's disease and neural developmental disorders. In Parkinson's disease the degeneration of dopaminergic neurons occurs where LMX1A plays an important role in neuronal development and maintenance showcasing its potential therapeutic significance. Furthermore downregulation of LMX1A can lead to a disrupted neuronal development illustrating its impact on diseases associated with neurological development while the protein NURR1 is also critically involved in these processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a transcriptional activator by binding to an A/T-rich sequence, the FLAT element, in the insulin gene promoter. Required for development of the roof plate and, in turn, for specification of dorsal cell fates in the CNS and developing vertebrae (By similarity).
See full target information LMX1A
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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