JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB272683

Anti-LMX1b antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal LMX1B antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LMX1B aa 1-100.

View Alternative Names

LIM homeobox transcription factor 1-beta, LIM/homeobox protein 1.2, LIM/homeobox protein LMX1B, LMX-1.2, LMX1B

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-LMX1b antibody (AB272683)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LMX1b antibody (AB272683)

PFA fixed, Triton X-100 permeablized MCF7 cells (Human breast adenocarcinoma cell line) labeling LMX1b (green) using ab272683 at 2 ug/ml in ICC/IF analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human LMX1B aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

O60663

style
left-column

Complementary Products

Primary Antibodies

AB137869

Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

  • 5
  • 9
Primary Antibodies

AB108422

Anti-FOXA2 antibody [EPR4466]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (AB108422)

  • 4
  • 11
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB305355

Anti-Nurr1 antibody [EPR24121-44] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Nurr1 antibody [EPR24121-44] - BSA and Azide free (Detector) (AB305355)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Syrian hamster": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LMX1b also known as LIM homeobox transcription factor 1 beta functions as a transcription factor critical for the development of various tissues. This protein has a mass of approximately 42 kDa. LMX1b is mainly expressed in tissues such as the limb bud kidney and central nervous system during developmental stages. It regulates gene expression by binding to specific DNA sequences facilitating the transcription of target genes vital for tissue specification.
Biological function summary

LMX1b plays a significant role in differentiation and patterning processes. It acts in a transcriptional complex to regulate morphogenetic genes in developing tissues. Specifically LMX1b regulates dorsal-ventral patterning in limbs and kidney development. Mutations in this protein can result in critical errors during embryogenesis reflecting its importance in organogenesis and maintaining tissue homeostasis.

Pathways

LMX1b is involved in essential developmental signaling pathways such as the Wnt and Shh pathways. It interacts with other proteins like LDB1 and ISL1 in these pathways influencing gene networks that dictate cell fate and tissue architecture. The Wnt signaling pathway in particular is important for limb bud development where LMX1b ensures proper dorsal structures highlighting its regulatory role in morphogenetic signaling.

LMX1b mutations have strong associations with Nail-Patella Syndrome and Focal Segmental Glomerulosclerosis. Nail-Patella Syndrome results from disrupted LMX1b function leading to abnormalities in limb and nail development. The protein BMPR1B collaborates with LMX1b in bone morphogenesis and mutations can exacerbate disorder symptoms. Understanding LMX1b’s interactions offers insights into potential therapeutic pathways for managing these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcription factor involved in the regulation of podocyte-expressed genes (PubMed : 24042019, PubMed : 28059119). Essential for the specification of dorsal limb fate at both the zeugopodal and autopodal levels.
See full target information LMX1B
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Genes 15: PubMed38927597

2024

Neuronal Ceroid Lipofuscinosis in a Mixed-Breed Dog with a Splice Site Variant in .

Applications

Unspecified application

Species

Unspecified reactive species

Tendai Mhlanga-Mutangadura,Garrett Bullock,Sofia Cerda-Gonzalez,Martin L Katz
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com