Anti-LMX1b antibody [EPR24053-161] (ab259926)

Rabbit Recombinant Monoclonal LMX1B antibody. Suitable for IP, Dot, WB, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.

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Images

Western blot - Anti-LMX1b antibody [EPR24053-161] (AB259926), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	IP	Dot	WB	IHC-P	IHC-Fr	Flow Cyt (Intra)
Human	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Not recommended	Tested	Tested	Tested	Tested	Not recommended	Not recommended
Rat	Not recommended	Expected	Expected	Tested	Tested	Not recommended	Not recommended

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/2000	Notes -
Species Rat	Dilution info 1/2000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Target data

See full target information Lmx1b

Function

Transcription factor involved in the regulation of podocyte-expressed genes. Essential for the specification of dorsal limb fate at both the zeugopodal and autopodal levels.

Alternative names

LIM homeobox transcription factor 1-beta, LIM/homeobox protein 1.2, LIM/homeobox protein LMX1B, LMX-1.2, Lmx1b

Recommended products

Rabbit Recombinant Monoclonal LMX1B antibody. Suitable for IP, Dot, WB, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR24053-161
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

LMX1b also known as LIM homeobox transcription factor 1 beta functions as a transcription factor critical for the development of various tissues. This protein has a mass of approximately 42 kDa. LMX1b is mainly expressed in tissues such as the limb bud kidney and central nervous system during developmental stages. It regulates gene expression by binding to specific DNA sequences facilitating the transcription of target genes vital for tissue specification.

Biological function summary

LMX1b plays a significant role in differentiation and patterning processes. It acts in a transcriptional complex to regulate morphogenetic genes in developing tissues. Specifically LMX1b regulates dorsal-ventral patterning in limbs and kidney development. Mutations in this protein can result in critical errors during embryogenesis reflecting its importance in organogenesis and maintaining tissue homeostasis.

Pathways

LMX1b is involved in essential developmental signaling pathways such as the Wnt and Shh pathways. It interacts with other proteins like LDB1 and ISL1 in these pathways influencing gene networks that dictate cell fate and tissue architecture. The Wnt signaling pathway in particular is important for limb bud development where LMX1b ensures proper dorsal structures highlighting its regulatory role in morphogenetic signaling.

Associated diseases and disorders

LMX1b mutations have strong associations with Nail-Patella Syndrome and Focal Segmental Glomerulosclerosis. Nail-Patella Syndrome results from disrupted LMX1b function leading to abnormalities in limb and nail development. The protein BMPR1B collaborates with LMX1b in bone morphogenesis and mutations can exacerbate disorder symptoms. Understanding LMX1b’s interactions offers insights into potential therapeutic pathways for managing these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-LMX1b antibody [EPR24053-161] (ab259926)
Blocking and diluting buffer and concentration: 5% NFDM/TBST Negative control: stomach and ovary (PMID: 24056967).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes
All lanes: Western blot - Anti-LMX1b antibody [EPR24053-161] (ab259926) at 1/1000 dilution
Lane 1: Mouse hypothalamus tissue lysate at 40 µg
Lane 2: Mouse stomach tissue lysate at 40 µg
Lane 3: Mouse ovary tissue lysate at 40 µg
Lane 4: Rat hypothalamus tissue lysate at 40 µg
Lane 5: Rat E17 embryo tissue lysate at 40 µg
Lane 6: Rat stomach tissue lysate at 40 µg
Secondary
All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 45 kDa
Western blot - Anti-LMX1b antibody [EPR24053-161] (ab259926)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody has a weak cross-reactivity with LMX1a.
Exposure time: 5.5 seconds
All lanes: Western blot - Anti-LMX1b antibody [EPR24053-161] (ab259926) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) transfected with mouse LMX1b full length expression vector containing a myc-His-tag®, whole cell lysate at 10 µg
Lane 2: E.coli extracts containing his-tagged mouse LMX1a full length recombinant protein at 20 µL
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1b antibody [EPR24053-161] (ab259926)
Immunohistochemical analysis of paraffin-embedded Mouse hypothalamus tissue labelling LMX1b with ab259926 at 1/2000 (0.278 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse hypothalamus (PMID: 27407143). The section was incubated with ab259926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1b antibody [EPR24053-161] (ab259926)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling LMX1b with ab259926 at 1/2000 (0.278 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on the glomerulus of mouse kidney. The section was incubated with ab259926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1b antibody [EPR24053-161] (ab259926)
Immunohistochemical analysis of paraffin-embedded Rat hypothalamus tissue labelling LMX1b with ab259926 at 1/2000 (0.278 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on rat hypothalamus. The section was incubated with ab259926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1b antibody [EPR24053-161] (ab259926)
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labelling LMX1b with ab259926 at 1/2000 (0.278 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on the glomerulus of rat kidney (PMID: 24785190). The section was incubated with ab259926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunoprecipitation - Anti-LMX1b antibody [EPR24053-161] (ab259926)
LMX1b was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) transfected with mouse LMX1b expression vector containing a myc-His-tag®, whole cell lysate 10 ug with ab259926 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259926 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HEK-293T (human embryonic kidney) transfected with mouse LMX1b expression vector containing a myc-His-tag®, whole cell lysate 10 ug
Lane 2: ab259926 IP in HEK-293T transfected with mouse LMX1b expression vector containing a myc-His-tag®, whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab259926 in HEK-293T transfected with mouse LMX1b expression vector containing a myc-His-tag®, whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
All lanes: Immunoprecipitation - Anti-LMX1b antibody [EPR24053-161] (ab259926)
Predicted band size: 42 kDa
Observed band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1b antibody [EPR24053-161] (ab259926)
Immunohistochemical analysis of paraffin-embedded Rat E14.5 embryo tissue labelling LMX1b with ab259926 at 1/2000 (0.278 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on the spinal cord of rat E14.5 embryo. The section was incubated with ab259926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1b antibody [EPR24053-161] (ab259926)
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labelling LMX1b with ab259926 at 1/2000 (0.278 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: almost no staining on mouse stomach. The section was incubated with ab259926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Dot Blot - Anti-LMX1b antibody [EPR24053-161] (ab259926)
Dot blot analysis of LMX1b using ab259926 at 1:1000 (0.556 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Exposure time: 3 minutes
Blocking and diluting buffer and concentration: 5% NFDM/TBST