Rabbit Recombinant Monoclonal LMX1B antibody. Suitable for IP, Dot, WB, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
ICC/IF | IP | Dot | WB | IHC-P | IHC-Fr | Flow Cyt (Intra) | |
---|---|---|---|---|---|---|---|
Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Mouse | Not recommended | Tested | Tested | Tested | Tested | Not recommended | Not recommended |
Rat | Not recommended | Expected | Expected | Tested | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/2000 | Notes - |
Species Rat | Dilution info 1/2000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Transcription factor involved in the regulation of podocyte-expressed genes. Essential for the specification of dorsal limb fate at both the zeugopodal and autopodal levels.
LIM homeobox transcription factor 1-beta, LIM/homeobox protein 1.2, LIM/homeobox protein LMX1B, LMX-1.2, Lmx1b
Rabbit Recombinant Monoclonal LMX1B antibody. Suitable for IP, Dot, WB, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
LMX1b also known as LIM homeobox transcription factor 1 beta functions as a transcription factor critical for the development of various tissues. This protein has a mass of approximately 42 kDa. LMX1b is mainly expressed in tissues such as the limb bud kidney and central nervous system during developmental stages. It regulates gene expression by binding to specific DNA sequences facilitating the transcription of target genes vital for tissue specification.
LMX1b plays a significant role in differentiation and patterning processes. It acts in a transcriptional complex to regulate morphogenetic genes in developing tissues. Specifically LMX1b regulates dorsal-ventral patterning in limbs and kidney development. Mutations in this protein can result in critical errors during embryogenesis reflecting its importance in organogenesis and maintaining tissue homeostasis.
LMX1b is involved in essential developmental signaling pathways such as the Wnt and Shh pathways. It interacts with other proteins like LDB1 and ISL1 in these pathways influencing gene networks that dictate cell fate and tissue architecture. The Wnt signaling pathway in particular is important for limb bud development where LMX1b ensures proper dorsal structures highlighting its regulatory role in morphogenetic signaling.
LMX1b mutations have strong associations with Nail-Patella Syndrome and Focal Segmental Glomerulosclerosis. Nail-Patella Syndrome results from disrupted LMX1b function leading to abnormalities in limb and nail development. The protein BMPR1B collaborates with LMX1b in bone morphogenesis and mutations can exacerbate disorder symptoms. Understanding LMX1b’s interactions offers insights into potential therapeutic pathways for managing these conditions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and diluting buffer and concentration: 5% NFDM/TBST Negative control: stomach and ovary (PMID: 24056967).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes
All lanes: Western blot - Anti-LMX1b antibody [EPR24053-161] (ab259926) at 1/1000 dilution
Lane 1: Mouse hypothalamus tissue lysate at 40 µg
Lane 2: Mouse stomach tissue lysate at 40 µg
Lane 3: Mouse ovary tissue lysate at 40 µg
Lane 4: Rat hypothalamus tissue lysate at 40 µg
Lane 5: Rat E17 embryo tissue lysate at 40 µg
Lane 6: Rat stomach tissue lysate at 40 µg
All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 45 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody has a weak cross-reactivity with LMX1a.
Exposure time: 5.5 seconds
All lanes: Western blot - Anti-LMX1b antibody [EPR24053-161] (ab259926) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) transfected with mouse LMX1b full length expression vector containing a myc-His-tag®, whole cell lysate at 10 µg
Lane 2: E.coli extracts containing his-tagged mouse LMX1a full length recombinant protein at 20 µL
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Immunohistochemical analysis of paraffin-embedded Mouse hypothalamus tissue labelling LMX1b with ab259926 at 1/2000 (0.278 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse hypothalamus (PMID: 27407143). The section was incubated with ab259926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling LMX1b with ab259926 at 1/2000 (0.278 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on the glomerulus of mouse kidney. The section was incubated with ab259926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded Rat hypothalamus tissue labelling LMX1b with ab259926 at 1/2000 (0.278 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on rat hypothalamus. The section was incubated with ab259926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labelling LMX1b with ab259926 at 1/2000 (0.278 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on the glomerulus of rat kidney (PMID: 24785190). The section was incubated with ab259926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
LMX1b was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) transfected with mouse LMX1b expression vector containing a myc-His-tag®, whole cell lysate 10 ug with ab259926 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259926 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HEK-293T (human embryonic kidney) transfected with mouse LMX1b expression vector containing a myc-His-tag®, whole cell lysate 10 ug
Lane 2: ab259926 IP in HEK-293T transfected with mouse LMX1b expression vector containing a myc-His-tag®, whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab259926 in HEK-293T transfected with mouse LMX1b expression vector containing a myc-His-tag®, whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
All lanes: Immunoprecipitation - Anti-LMX1b antibody [EPR24053-161] (ab259926)
Predicted band size: 42 kDa
Observed band size: 45 kDa
Immunohistochemical analysis of paraffin-embedded Rat E14.5 embryo tissue labelling LMX1b with ab259926 at 1/2000 (0.278 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on the spinal cord of rat E14.5 embryo. The section was incubated with ab259926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labelling LMX1b with ab259926 at 1/2000 (0.278 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: almost no staining on mouse stomach. The section was incubated with ab259926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Dot blot analysis of LMX1b using ab259926 at 1:1000 (0.556 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Exposure time: 3 minutes
Blocking and diluting buffer and concentration: 5% NFDM/TBST
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