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AB244278

Anti-LNK antibody

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(2 Publications)

Rabbit Polyclonal LNK antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SH2B3 aa 200-400.

View Alternative Names

LNK, SH2B3, SH2B adapter protein 3, Lymphocyte adapter protein, Lymphocyte-specific adapter protein Lnk, Signal transduction protein Lnk

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-LNK antibody (AB244278)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LNK antibody (AB244278)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for LNK (green) using ab244278 at 4 μg/ml in ICC/IF.

Western blot - Anti-LNK antibody (AB244278)
  • WB

Unknown

Western blot - Anti-LNK antibody (AB244278)

All lanes:

Western blot - Anti-LNK antibody (ab244278) at 0.4 µg/mL

All lanes:

HEL (human erythroleukemia cell line) cell lysate

Predicted band size: 63 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SH2B3 aa 200-400. The exact immunogen used to generate this antibody is proprietary information.

Q9UQQ2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LNK also known as SH2B3 is an adaptor protein about 70 kDa in mass. LNK contains an SH2 domain and is expressed mainly in hematopoietic cells including stem and progenitor cells. This protein inhibits signaling pathways by binding with receptors and blocking further transduction. It works by negatively regulating signals mediated by cytokine receptors and receptor tyrosine kinases.
Biological function summary

The LNK protein functions as a negative regulator in the hematopoietic system helping to control cell proliferation and immune response. LNK interacts with other signaling proteins as part of regulatory complexes often utilizing its SH2 domain to bind phosphorylated tyrosines. By doing so it inhibits the activity of various kinases involved in cell division ensuring proper signaling and growth control. This role is important to maintaining cellular homeostasis and preventing excessive cell proliferation.

Pathways

The LNK protein plays significant roles in the JAK-STAT and PI3K-AKT signaling pathways. In these pathways LNK interacts with signaling molecules like JAK2 and STAT5 to limit excessive signaling. This interaction is known to affect cellular responses to growth factors and cytokines impacting processes such as cell survival and proliferation. Through its activity in these pathways LNK helps ensure appropriate cellular responses to external stimuli.

LNK has connections with conditions like myeloproliferative neoplasms and hypertension. Mutations or aberrant expression of LNK can lead to dysregulated signaling that contributes to these diseases. In myeloproliferative neoplasms altered LNK function can lead to uncontrolled cell proliferation through interactions with proteins such as JAK2. In hypertension its role in regulating vascular tone and inflammation highlights its potential involvement in the pathogenesis of the disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Links T-cell receptor activation signal to phospholipase C-gamma-1, GRB2 and phosphatidylinositol 3-kinase.
See full target information SH2B3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:114309 PubMed38848215

2024

Lineage specification in glioblastoma is regulated by METTL7B.

Applications

Unspecified application

Species

Unspecified reactive species

Myrianni Constantinou,James Nicholson,Xinyu Zhang,Eleni Maniati,Sara Lucchini,Gabriel Rosser,Claire Vinel,Jun Wang,Yau Mun Lim,Sebastian Brandner,Sven Nelander,Sara Badodi,Silvia Marino

Aging 12:17150-17166 PubMed32911464

2020

LNK deficiency decreases obesity-induced insulin resistance by regulating GLUT4 through the PI3K-Akt-AS160 pathway in adipose tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaozhu Zhong,Chuanfeng Ke,Zhaoxi Cai,Hao Wu,Yang Ye,Xiaolin Liang,Liqun Yu,Sushi Jiang,Jun Shen,Laiyou Wang,Meiqing Xie,Guanlei Wang,Xiaomiao Zhao
View all publications
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