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AB249151

Anti-LNK antibody [EPR7342] - BSA and Azide free

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Rabbit Recombinant Monoclonal LNK antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

LNK, SH2B3, SH2B adapter protein 3, Lymphocyte adapter protein, Lymphocyte-specific adapter protein Lnk, Signal transduction protein Lnk

1 Images
Western blot - Anti-LNK antibody [EPR7342] - BSA and Azide free (AB249151)
  • WB

Unknown

Western blot - Anti-LNK antibody [EPR7342] - BSA and Azide free (AB249151)

This data was developed using ab154848, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LNK antibody [EPR7342] (<a href='/en-us/products/primary-antibodies/lnk-antibody-epr7342-ab154848'>ab154848</a>) at 1/1000 dilution

Lane 1:

Ramos cell lysate at 10 µg

Lane 2:

MOLT4 cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Predicted band size: 63 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7342

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249151 is the carrier-free version of ab154848.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LNK also known as SH2B3 is an adaptor protein about 70 kDa in mass. LNK contains an SH2 domain and is expressed mainly in hematopoietic cells including stem and progenitor cells. This protein inhibits signaling pathways by binding with receptors and blocking further transduction. It works by negatively regulating signals mediated by cytokine receptors and receptor tyrosine kinases.
Biological function summary

The LNK protein functions as a negative regulator in the hematopoietic system helping to control cell proliferation and immune response. LNK interacts with other signaling proteins as part of regulatory complexes often utilizing its SH2 domain to bind phosphorylated tyrosines. By doing so it inhibits the activity of various kinases involved in cell division ensuring proper signaling and growth control. This role is important to maintaining cellular homeostasis and preventing excessive cell proliferation.

Pathways

The LNK protein plays significant roles in the JAK-STAT and PI3K-AKT signaling pathways. In these pathways LNK interacts with signaling molecules like JAK2 and STAT5 to limit excessive signaling. This interaction is known to affect cellular responses to growth factors and cytokines impacting processes such as cell survival and proliferation. Through its activity in these pathways LNK helps ensure appropriate cellular responses to external stimuli.

LNK has connections with conditions like myeloproliferative neoplasms and hypertension. Mutations or aberrant expression of LNK can lead to dysregulated signaling that contributes to these diseases. In myeloproliferative neoplasms altered LNK function can lead to uncontrolled cell proliferation through interactions with proteins such as JAK2. In hypertension its role in regulating vascular tone and inflammation highlights its potential involvement in the pathogenesis of the disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Links T-cell receptor activation signal to phospholipase C-gamma-1, GRB2 and phosphatidylinositol 3-kinase.
See full target information SH2B3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com