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AB121416

Anti-LNP antibody

4

(2 Reviews)

|

(2 Publications)

Rabbit Polyclonal LNP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LNPK aa 250-400.

View Alternative Names

KIAA1715, LNP, LNPK, Endoplasmic reticulum junction formation protein lunapark, ER junction formation factor lunapark

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LNP antibody (AB121416)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LNP antibody (AB121416)

Immunohistochemical analysis of human tonsil tissue labeling LNP with ab121416 at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LNP antibody (AB121416)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LNP antibody (AB121416)

Immunohistochemical analysis of human cervix tissue labeling LNP with ab121416 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-LNP antibody (AB121416)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LNP antibody (AB121416)

Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121416 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LNP antibody (AB121416)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LNP antibody (AB121416)

Immunohistochemical analysis of human testis tissue labeling LNP with ab121416 at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LNP antibody (AB121416)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LNP antibody (AB121416)

Immunohistochemical analysis of human cerebellum tissue labeling LNP with ab121416 at 1/500 dilution.

Western blot - Anti-LNP antibody (AB121416)
  • WB

Supplier Data

Western blot - Anti-LNP antibody (AB121416)

Loading Control : Anti-GAPDH

All lanes:

Western blot - Anti-LNP antibody (ab121416) at 0.4 µg/mL

Lane 1:

U-138 MG (human glioblastoma cell line) transfected with control siRNA, whole cell lysate

Lane 2:

U-138 MG transfected with target specific siRNA probe #1, whole cell lysate

Lane 3:

U-138 MG transfected with target specific siRNA probe #2, whole cell lysate

Predicted band size: 48 kDa

false

Western blot - Anti-LNP antibody (AB121416)
  • WB

Supplier Data

Western blot - Anti-LNP antibody (AB121416)

Western blot analysis of human U-138 MG cell lysate labeling LNP with ab121416 at 0.04 ug/ml.

All lanes:

Western blot - Anti-LNP antibody (ab121416) at 0.04 µg/mL

All lanes:

U-138 MG cell lysate

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human LNPK aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

Q9C0E8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lipid nanoparticles (LNPs) serve as important carriers in drug delivery systems especially for mRNA vaccines. They are composed of lipids and possess an average mass that varies depending on the lipid composition and payload they carry. LNPs are often used to encapsulate nucleic acids such as mRNA providing protection and enhancing cellular uptake. Expression of LNP content localizes primarily to sites where mRNA vaccines are administered delivering cargo into target cells efficiently.
Biological function summary

Lipid nanoparticles facilitate the delivery and release of payloads like mRNA inside cells. They do not inherently form part of a biological complex but when combined with mRNA they act as a pseudo-complex that helps ensure the stability and proper cellular entry of the genetic material. LNPs utilize endocytic pathways for cellular uptake where they eventually release their payload into the cytosol through endosomal escape mechanisms.

Pathways

One should note that LNPs significantly engage in endocytosis a critical cellular uptake pathway. Through their delivery mechanism they interact indirectly with proteins involved in membrane fusion and endosomal escape such as SNARE proteins and proton pumps that regulate endosomal pH levels. Moreover when carrying mRNA constructs they indirectly participate in the protein translation pathways by facilitating the mRNA's availability in the cytosol for ribosomes to function.

Lipid nanoparticles have revolutionized vaccine development prominently evidenced by their role in mRNA COVID-19 vaccines. They relate to the efficacy of vaccine-induced immune responses. Conditions such as viral infections and genetic disorders have therefore seen therapeutic advancements through the use of LNPs linked to the successful delivery of mRNA sequences coding for immunogenic proteins or therapeutic peptides. In these cases proteins like spike protein (from SARS-CoV-2) become the focus of the robust immune response facilitated through LNP-mediated delivery systems.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Endoplasmic reticulum (ER)-shaping membrane protein that plays a role in determining ER morphology (PubMed : 30032983). Involved in the stabilization of nascent three-way ER tubular junctions within the ER network (PubMed : 24223779, PubMed : 25404289, PubMed : 25548161, PubMed : 27619977). May also play a role as a curvature-stabilizing protein within the three-way ER tubular junction network (PubMed : 25404289). May be involved in limb development (By similarity). Is involved in central nervous system development (PubMed : 30032983).
See full target information LNPK
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 82:585-597.e11 PubMed35120648

2022

Co-adaptor driven assembly of a CUL3 E3 ligase complex.

Applications

Unspecified application

Species

Unspecified reactive species

David Akopian,Colleen A McGourty,Michael Rapé

Cell 167:525-538.e14 PubMed27716508

2016

Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor.

Applications

Unspecified application

Species

Unspecified reactive species

Colleen A McGourty,David Akopian,Carolyn Walsh,Amita Gorur,Achim Werner,Randy Schekman,Diana Bautista,Michael Rape
View all publications
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