AB190255

Anti-LNPEP antibody - N-terminal

Name: Anti-LNPEP antibody - N-terminal (ab190255) | Abcam
Brand: Abcam Limited
SKU: AB190255
Price: 520 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal LNPEP antibody. N-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human LNPEP aa 1-50.

View Alternative Names

OTASE, LNPEP, Leucyl-cystinyl aminopeptidase, Cystinyl aminopeptidase, Insulin-regulated membrane aminopeptidase, Insulin-responsive aminopeptidase, Oxytocinase, Placental leucine aminopeptidase, IRAP, OTase, P-LAP

2 Images

Supplier Data

Immunoprecipitation - Anti-LNPEP antibody - N-terminal (AB190255)

Detection of LNPEP by Western Blot of Immunoprecipitate.
ab190255 at 1 μg/ml labeling LNPEP in Jurkat whole cell lysate immunoprecipitated using ab190255 at 6 μg/reaction (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-LNPEP antibody - N-terminal (ab190255)

Predicted band size: 117 kDa

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Supplier Data

Western blot - Anti-LNPEP antibody - N-terminal (AB190255)

All lanes:

Western blot - Anti-LNPEP antibody - N-terminal (ab190255) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 117 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human LNPEP aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9UIQ6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Common marmoset": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab190255 was affinity purified using an epitope specific to LNPEP immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LNPEP also called leucyl/cystinyl aminopeptidase or insulin-regulated membrane aminopeptidase (IRAP) serves as a zinc-dependent aminopeptidase. This protein cleaves N-terminal amino acids from peptide substrates functioning inside various tissues like the heart brain and placenta. The mass of LNPEP is approximately 140 kDa. It associates with the endosome compartment in cells and resides at the plasma membrane linking to vesicular transport processes.

Biological function summary

LNPEP plays a role in peptide hormone regulation and recognition involving itself in glucose homeostasis and blood pressure regulation. It joins part of complexes involved in peptide recycling and processing within endocytic vesicles. This protein regulates the degradation of peptide hormones like oxytocin and vasopressin indicating its function extends to neuropeptide modulation.

Pathways

The activity of LNPEP spans significant biological pathways such as the renin-angiotensin system where it modulates the levels of angiotensin IV impacting cardiovascular functions and influencing blood pressure regulation. It closely relates to proteins like insulin-responsive aminopeptidase and angiotensin IV receptor enhancing its regulatory role within these pathways.

LNPEP significantly links to hypertension and diabetes. The modulation of angiotensin IV by LNPEP directly impacts blood pressure associating its activity with the development and management of hypertension. Additionally LNPEP's impact on glucose uptake and insulin action connects it with diabetic conditions. It exhibits interactions with proteins such as the glucose transporter type 4 (GLUT4) in the context of diabetes highlighting its role in metabolic regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Release of an N-terminal amino acid, cleaves before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain.

See full target information LNPEP

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:199 PubMed40399808

2025

Metformin reverses 5-FU resistance induced by radiotherapy through mediating folate metabolism in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shuxuan Wang,Yanyan Lin,Qianqian Zhao,Huanliang Chen,Shisuo Du,Zhaochong Zeng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LNPEP antibody - N-terminal

Immunoprecipitation - Anti-LNPEP antibody - N-terminal (AB190255)

Western blot - Anti-LNPEP antibody - N-terminal (AB190255)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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