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Rabbit Recombinant Multiclonal LNX2 antibody. Suitable for ICC/IF, WB and reacts with Human samples.

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Images

Western blot - Anti-LNX2 antibody [RP23040098] (AB313433), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-LNX2 antibody [RP23040098] (AB313433), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form
Liquid
Clonality
Multiclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
1.00000-3.00000 µg/mL
Notes

-

Target data

Rabbit Recombinant Multiclonal LNX2 antibody. Suitable for ICC/IF, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Clone number
RP23040098
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.

Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LNX2 also known as Ligand of Numb-protein X 2 is an E3 ubiquitin-protein ligase that plays a central role in controlling protein degradation. The molecular mass of LNX2 is approximately 73 kDa. This protein commonly appears in tissues such as the brain and liver. LNX2 contains several PDZ domains which facilitate protein-protein interactions and it is involved in the ubiquitination pathway marking proteins for degradation.

Biological function summary

LNX2 interacts with other proteins to regulate cellular signaling processes. LNX2 often forms part of a larger protein complex that includes Numb a protein involved in cell fate determination. Through these interactions LNX2 modulates important cellular functions like endocytosis and cell signaling important for maintaining cellular homeostasis. LNX2's role in interacting with several proteins indicates its importance in cellular regulatory networks.

Pathways

LNX2 significantly contributes to the Notch signaling pathway which regulates cell differentiation and proliferation. LNX2 interacts with the Numb protein within this pathway influencing the outcome of signal transduction. Additionally LNX2 has roles within the Wnt signaling pathway another critical pathway in cell fate decisions and embryonic development. These pathways highlight LNX2's involvement in regulating key cellular processes in developmental biology.

Associated diseases and disorders

LNX2 has associations with cancers particularly in the brain and liver where its expression is prominent. Alterations in LNX2 expression or function may disrupt the balance of cellular signaling pathways and contribute to tumorigenesis. Additionally LNX2 has connections with neurological disorders potentially due to its regulation of Numb and subsequent effects on neurodevelopmental pathways. LNX2's involvement in these diseases underlines its potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-LNX2 antibody [RP23040098] (ab313433), expandable thumbnail

    Western blot - Anti-LNX2 antibody [RP23040098] (ab313433)

    All lanes: Western blot - Anti-LNX2 antibody [RP23040098] (ab313433) at 1 µg/mL

    Lane 1: K562 whole cell lysate at 50 µg

    Lane 2: Jurkat whole cell lysate at 50 µg

    Lane 3: HT-29 whole cell lysate at 50 µg

    Lane 4: HeLa whole cell lysate at 50 µg

    Lane 5: PANC-1 whole cell lysate at 50 µg

    Lane 6: HCT116 whole cell lysate at 50 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 76.004 kDa

    Observed band size: 65 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-LNX2 antibody [RP23040098] (ab313433), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-LNX2 antibody [RP23040098] (ab313433)

    Immunofluorescent analysis of A-431 cells fixed using 4% formaldehyde (reconstituted in 1X PBS) for 10 min at room temperature and permeabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling LNX2 with ab313433 at 1 ?g/mL followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2500 dilution. Panel a) shows representative cells that were stained for detection and localization of LNX2 protein (green), Panel b) is stained for nuclei (blue) using DAPI at 1/50 dilution. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor 594 Phalloidin at 1/200 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of LNX2. Panel e) represents control cells with no primary antibody to assess background.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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