Anti-LNX2 antibody [RP23040098]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LNX2 antibody [RP23040098] (AB313433)

Immunofluorescent analysis of A-431 cells fixed using 4% formaldehyde (reconstituted in 1X PBS) for 10 min at room temperature and permeabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling LNX2 with ab313433 at 1 ?g/mL followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2500 dilution. Panel a) shows representative cells that were stained for detection and localization of LNX2 protein (green), Panel b) is stained for nuclei (blue) using DAPI at 1/50 dilution. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor 594 Phalloidin at 1/200 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of LNX2. Panel e) represents control cells with no primary antibody to assess background.

• WB

Supplier Data

Western blot - Anti-LNX2 antibody [RP23040098] (AB313433)

All lanes:

Western blot - Anti-LNX2 antibody [RP23040098] (ab313433) at 1 µg/mL

Lane 1:

K562 whole cell lysate at 50 µg

Lane 2:

Jurkat whole cell lysate at 50 µg

Lane 3:

HT-29 whole cell lysate at 50 µg

Lane 4:

HeLa whole cell lysate at 50 µg

Lane 5:

PANC-1 whole cell lysate at 50 µg

Lane 6:

HCT116 whole cell lysate at 50 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate at 1/2500 dilution

Predicted band size: 76.004 kDa

Observed band size: 65 kDa

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