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AB237694

Anti-LOC134147/CMBL antibody

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(1 Publication)

Rabbit Polyclonal LOC134147/CMBL antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CMBL.

View Alternative Names

Carboxymethylenebutenolidase homolog, CMBL

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOC134147/CMBL antibody (AB237694)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOC134147/CMBL antibody (AB237694)

Paraffin-embedded human small intestine tissue stained for LOC134147/CMBL with ab237694 at a 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-LOC134147/CMBL antibody (AB237694)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LOC134147/CMBL antibody (AB237694)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for LOC134147/CMBL (Green) using ab237694 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOC134147/CMBL antibody (AB237694)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOC134147/CMBL antibody (AB237694)

Paraffin-embedded human kidney tissue stained for LOC134147/CMBL with ab237694 at a 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CMBL.

Q96DG6

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Complementary Products

Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiphysin antibody [EP2061Y] (AB76124)

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Proteins & Peptides

AB130050

Recombinant Human LOC134147/CMBL protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human LOC134147/CMBL protein (His tag N-Terminus) (AB130050)

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Assay Kits

AB126286

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Assay Kits

AB189576

Human Apolipoprotein AI ELISA Kit

SimpleStep

ELISA - Human Apolipoprotein AI ELISA Kit (AB189576)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LOC134147 often called CMBL (carboxymethylenebutenolidase-like protein) functions as an enzyme with a pivotal role in detoxification processes. This protein weighs approximately 30 kDa and exhibits carboxymethylenebutenolidase activity catalyzing the hydrolysis of carboxymethylenebutenolide to produce carboxymethylenebutenolide lactone and an alcohol. Expression of CMBL occurs in various tissues but it is highly present in the liver indicating its connection to metabolic processes in detoxification and drug metabolism.
Biological function summary

CMBL facilitates the efficient breakdown of certain xenobiotics and endogenous compounds contributing to cellular detoxification mechanisms. As a standalone enzyme it does not participate in large protein complexes but its activity assists the liver in processing numerous therapeutic drugs and harmful compounds. The liver's expression of CMBL highlights its role in maintaining metabolic balance and cellular homeostasis against various chemical exposures.

Pathways

The function of CMBL fits into the broader context of metabolic and detoxification pathways specifically xenobiotic metabolism and retinoic acid biosynthesis. CMBL is actively involved in the hydrolysis step working alongside enzymes like aldehyde dehydrogenase in detoxification processes. This association enables efficient processing and neutralization of exogenous substances aiding in the maintenance of chemical homeostasis within the human body.

Alterations in CMBL activity can affect disease states notably impacting drug efficacy and safety. Anomalous function of CMBL is suspected to be connected with variations in drug response including drug-induced liver injury. Through drug metabolic pathways it indirectly interacts with proteins like cytochrome P450 which also modulate drug metabolism and detoxification influencing therapeutic outcomes. Understanding CMBL's role aids in improving drug safety and personalized medicine strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cysteine hydrolase whose endogenous substrates and physiological function are unknown. Catalyzes the hydrolytic activation of several ester-based prodrugs, by cleaving the dioxolone ring to yield their respective active metabolites, diacetyl and CO2. Can thus convert the prodrugs Olmesartan medoxomil and Azilsartan medoxomil into their active metabolites (Olmesartan and Azilsartan), two angiotensin receptor blockers. Is also able to activate beta-lactam antibiotics Faropenem medoxomil and Lenampicillin.
See full target information CMBL
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 299:104982 PubMed37390992

2023

Persistent ER stress causes GPI anchor deficit to convert a GPI-anchored prion protein into pro-PrP via the ATF6-miR449c-5p-PIGV axis.

Applications

Unspecified application

Species

Unspecified reactive species

JingFeng Li,SaSa Li,ShuPei Yu,Jie Yang,JingRu Ke,Huan Li,Heng Chen,MingJian Lu,Man-Sun Sy,ZhenXing Gao,Chaoyang Li
View all publications
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Product promise

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