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AB70484

Anti-LOK antibody

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(3 Publications)

Rabbit Polyclonal LOK antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human STK10 aa 900 to C-terminus.

View Alternative Names

LOK, STK10, Serine/threonine-protein kinase 10, Lymphocyte-oriented kinase

2 Images
Immunoprecipitation - Anti-LOK antibody (AB70484)
  • IP

Unknown

Immunoprecipitation - Anti-LOK antibody (AB70484)

Detection of Human LOK by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP), using ab70484 at 1 µg/mg lysate for IP.
Lane 1 : input of Human LOK at 50μg
Lane 2 : Other anti-human LOK antibody
Lane 3 : Other anti-human LOK antibody plus blocking peptide
Lane 4 : ab70484
Lane 5 : ab70484 plus blocking peptide
Subsequent Western blot LOK detection was performed with 0.1 µg/mg ab70484.
Chemiluminescence with exposure times of 1 to 2 minutes.

All lanes:

Immunoprecipitation - Anti-LOK antibody (ab70484)

Predicted band size: 112 kDa

false

Western blot - Anti-LOK antibody (AB70484)
  • WB

Unknown

Western blot - Anti-LOK antibody (AB70484)

Chemiluminescence with exposure times of 1 to 2 minutes.

All lanes:

Western blot - Anti-LOK antibody (ab70484) at 0.1 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 5 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 50 µg

Predicted band size: 112 kDa

Observed band size: 112 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human STK10 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O94804

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1-4 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/15000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase involved in regulation of lymphocyte migration. Phosphorylates MSN, and possibly PLK1. Involved in regulation of lymphocyte migration by mediating phosphorylation of ERM proteins such as MSN. Acts as a negative regulator of MAP3K1/MEKK1. May also act as a cell cycle regulator by acting as a polo kinase kinase : mediates phosphorylation of PLK1 in vitro; however such data require additional evidences in vivo.
See full target information STK10
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

FEBS open bio 14:1087-1100 PubMed38720471

2024

HELQ deficiency impairs the induction of primordial germ cell-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Wan,Yaping Huang,Xingguo Xue,Gang Chang,Mei Wang,Xiao-Yang Zhao,Fang Luo,Zhi-Zhong Tang

Experimental and therapeutic medicine 22:851 PubMed34149897

2021

STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Zhang,Shun-Yuan Lu,Rui Guo,Jin-Xia Ma,Ling-Yun Tang,Jin-Jin Wang,Chun-Ling Shen,Li-Ming Lu,Jie Liu,Zhu-Gang Wang,Hong-Xin Zhang

Translational cancer research 9:7079-7090 PubMed35117313

2020

Knockout of promotes the migration and invasion of cervical cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Zhang,Shun-Yuan Lu,Rui Guo,Jin-Xia Ma,Ling-Yun Tang,Yan Shen,Chun-Ling Shen,Li-Ming Lu,Zhu-Gang Wang,Jie Liu,Hong-Xin Zhang
View all publications
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Product promise

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