AB103809

Anti-LONP1/Lon antibody

Name: Anti-LONP1/Lon antibody (ab103809) | Abcam
Brand: Abcam Limited
SKU: AB103809
Price: 535 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(35 Publications)

Rabbit Polyclonal LONP1/Lon antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 35 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LONP1.

View Alternative Names

PRSS15, LONP1, LONHs, Lon protease-like protein, Mitochondrial ATP-dependent protease Lon, Serine protease 15, LONP

5 Images

Supplier Data

Western blot - Anti-LONP1/Lon antibody (AB103809)

All lanes:

Western blot - Anti-LONP1/Lon antibody (ab103809) at 5 µg/mL

All lanes:

HepG2 cell lysate at 50 µg

Predicted band size: 106 kDa

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Supplier Data

Western blot - Anti-LONP1/Lon antibody (AB103809)

All lanes:

Western blot - Anti-LONP1/Lon antibody (ab103809) at 5 µg/mL

Lane 1:

LONP1 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Predicted band size: 106 kDa

false

Supplier Data

Western blot - Anti-LONP1/Lon antibody (AB103809)

All lanes:

Western blot - Anti-LONP1/Lon antibody (ab103809) at 5 µg/mL

All lanes:

Human kidney tissue lysate at 50 µg

Predicted band size: 104 kDa,106 kDa,117 kDa

Observed band size: 125 kDa,135 kDa

false

Supplier Data

Western blot - Anti-LONP1/Lon antibody (AB103809)

All lanes:

Western blot - Anti-LONP1/Lon antibody (ab103809) at 5 µg/mL

All lanes:

Mouse kidney tissue lysate at 50 µg

Predicted band size: 106 kDa

false

CiteAb

Western blot - Anti-LONP1/Lon antibody (AB103809)

LONP1/Lon western blot using anti-LONP1/Lon antibody ab103809. Publication image and figure legend from Kühl, I., Miranda, M., et al., 2017, Elife, PubMed 29132502.

ab103809 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab103809 please see the product overview.

Immunoblot of several mitochondrial proteins in extracts from L/L, cre and L/L hearts; Loading of 6 different membranes : SDHA, SDHB or VDAC1.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human LONP1.

P36776

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix (PubMed : 12198491, PubMed : 15870080, PubMed : 17579211, PubMed : 37327776, PubMed : 8248235). Endogenous substrates include mitochondrial steroidogenic acute regulatory (StAR) protein, DELE1, helicase Twinkle (TWNK) and the large ribosomal subunit protein MRPL32/bL32m (PubMed : 17579211, PubMed : 28377575, PubMed : 37327776). MRPL32/bL32m is protected from degradation by LONP1 when it is bound to a nucleic acid (RNA), but TWNK is not (PubMed : 17579211, PubMed : 28377575). May also have a chaperone function in the assembly of inner membrane protein complexes (By similarity). Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome (PubMed : 17420247). Binds to mitochondrial promoters and RNA in a single-stranded, site-specific, and strand-specific manner (PubMed : 17420247). May regulate mitochondrial DNA replication and/or gene expression using site-specific, single-stranded DNA binding to target the degradation of regulatory proteins binding to adjacent sites in mitochondrial promoters (PubMed : 14739292, PubMed : 17420247).

See full target information LONP1

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Publications (35)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:3221 PubMed40185737

2025

The Futile Creatine Cycle powers UCP1-independent thermogenesis in classical BAT.

Applications

Unspecified application

Species

Unspecified reactive species

Jakub Bunk,Mohammed F Hussain,Maria Delgado-Martin,Bozena Samborska,Mina Ersin,Abhirup Shaw,Janane F Rahbani,Lawrence Kazak

Cellular and molecular life sciences : CMLS 80:258 PubMed37594630

2023

Protease-independent control of parthanatos by HtrA2/Omi.

Applications

Unspecified application

Species

Unspecified reactive species

Jonas Weiß,Michelle Heib,Thiemo Korn,Justus Hoyer,Johaiber Fuchslocher Chico,Susann Voigt,Tomas Koudelka,Andreas Tholey,Dieter Adam

Cardiovascular research 119:2213-2229 PubMed37395010

2023

Tissue-specific differences in the assembly of mitochondrial Complex I are revealed by a novel ENU mutation in ECSIT.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Nicol,Sara Falcone,Andrew Blease,Pratik Vikhe,Gabriele Civiletto,Saleh Salman Omairi,Carlo Viscomi,Ketan Patel,Paul K Potter

iScience 26:107136 PubMed37408687

2023

Glutamine supplementation reverses manganese neurotoxicity by eliciting the mitochondrial unfolded protein response.

Applications

Unspecified application

Species

Unspecified reactive species

Shixuan Zhang,Junrou Zhang,Luli Wu,Li Chen,Piye Niu,Jie Li

Cells 11: PubMed36497197

2022

Differential Expression of Lonp1 Isoforms in Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Giada Zanini,Valentina Selleri,Anna De Gaetano,Lara Gibellini,Mara Malerba,Anna Vittoria Mattioli,Milena Nasi,Nadezda Apostolova,Marcello Pinti

Nature metabolism 4:1369-1401 PubMed36217034

2022

The aldolase inhibitor aldometanib mimics glucose starvation to activate lysosomal AMPK.

Applications

Unspecified application

Species

Unspecified reactive species

Chen-Song Zhang,Mengqi Li,Yu Wang,Xiaoyang Li,Yue Zong,Shating Long,Mingliang Zhang,Jin-Wei Feng,Xiaoyan Wei,Yan-Hui Liu,Baoding Zhang,Jianfeng Wu,Cixiong Zhang,Wenhua Lian,Teng Ma,Xiao Tian,Qi Qu,Yaxin Yu,Jinye Xiong,Dong-Tai Liu,Zhenhua Wu,Mingxia Zhu,Changchuan Xie,Yaying Wu,Zheni Xu,Chunyan Yang,Junjie Chen,Guohong Huang,Qingxia He,Xi Huang,Lei Zhang,Xiufeng Sun,Qingfeng Liu,Abdul Ghafoor,Fu Gui,Kaili Zheng,Wen Wang,Zhi-Chao Wang,Yong Yu,Qingliang Zhao,Shu-Yong Lin,Zhi-Xin Wang,Hai-Long Piao,Xianming Deng,Sheng-Cai Lin

Journal of lipid research 63:100279 PubMed36100091

2022

ER Stress-Induced Sphingosine-1-Phosphate Lyase Phosphorylation Potentiates the Mitochondrial Unfolded Protein Response.

Applications

Unspecified application

Species

Unspecified reactive species

Asli D Yildirim,Mevlut Citir,Asli E Dogan,Zehra Veli,Zehra Yildirim,Ozlem Tufanli,Alexis Traynor-Kaplan,Carsten Schultz,Ebru Erbay

Nature metabolism 4:739-758 PubMed35760869

2022

Impaired oxygen-sensitive regulation of mitochondrial biogenesis within the von Hippel-Lindau syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Shuijie Li,Wenyu Li,Juan Yuan,Petra Bullova,Jieyu Wu,Xuepei Zhang,Yong Liu,Monika Plescher,Javier Rodriguez,Oscar C Bedoya-Reina,Paulo R Jannig,Paula Valente-Silva,Meng Yu,Marie Arsenian Henriksson,Roman A Zubarev,Anna Smed-Sörensen,Carolyn K Suzuki,Jorge L Ruas,Johan Holmberg,Catharina Larsson,C Christofer Juhlin,Alex von Kriegsheim,Yihai Cao,Susanne Schlisio

Frontiers in pharmacology 13:859978 PubMed35652041

2022

Rosmarinic Acid Inhibits Mitochondrial Damage by Alleviating Unfolded Protein Response.

Applications

Unspecified application

Species

Unspecified reactive species

Guoen Cai,Fabin Lin,Dihang Wu,Chenxin Lin,Huiyun Chen,Yicong Wei,Huidan Weng,Zhiting Chen,Minxia Wu,En Huang,Zucheng Ye,Qinyong Ye

Cell reports 38:110475 PubMed35263592

2022

Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole M Sayles,Nneka Southwell,Kevin McAvoy,Kihwan Kim,Alba Pesini,Corey J Anderson,Catarina Quinzii,Suzanne Cloonan,Hibiki Kawamata,Giovanni Manfredi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-LONP1/Lon antibody

Western blot - Anti-LONP1/Lon antibody (AB103809)

Western blot - Anti-LONP1/Lon antibody (AB103809)

Western blot - Anti-LONP1/Lon antibody (AB103809)

Western blot - Anti-LONP1/Lon antibody (AB103809)

Western blot - Anti-LONP1/Lon antibody (AB103809)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (35)

Product promise