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Rabbit Polyclonal LONP1/Lon antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LONP1 aa 350-550.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human LONP1 aa 350-550. The exact immunogen used to generate this antibody is proprietary information. Database link P36776
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected
Cow
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/1000.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Species

Rat

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Species

Human

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Target data

Function

ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial promoters and RNA in a single-stranded, site-specific, and strand-specific manner. May regulate mitochondrial DNA replication and/or gene expression using site-specific, single-stranded DNA binding to target the degradation of regulatory proteins binding to adjacent sites in mitochondrial promoters (PubMed:12198491, PubMed:15870080, PubMed:17420247, PubMed:8248235). Endogenous substrates include mitochondrial steroidogenic acute regulatory (StAR) protein, helicase Twinkle (TWNK) and the large ribosomal subunit protein bL32m. bL32m is protected from degradation by LONP1 when it is bound to a nucleic acid (RNA), but TWNK is not (PubMed:17579211, PubMed:28377575).

Alternative names

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Rabbit Polyclonal LONP1/Lon antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LONP1 aa 350-550.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human LONP1 aa 350-550. The exact immunogen used to generate this antibody is proprietary information. Database link P36776
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

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6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody (ab224316), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody (ab224316)

    Paraffin embedded human duodenum tissue stained for LONP1/Lon with ab224316 (1/500 dilution) in immunohistochemical analysis.

  • Western blot - Anti-LONP1/Lon antibody (ab224316), expandable thumbnail

    Western blot - Anti-LONP1/Lon antibody (ab224316)

    All lanes: Western blot - Anti-LONP1/Lon antibody (AB224316) at 1/100 dilution

    All lanes: SK-MEL-30 cell lysate

    Predicted band size: 106 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody (ab224316), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody (ab224316)

    Paraffin embedded human tonsil tissue stained for LONP1/Lon with ab224316 (1/500 dilution) in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-LONP1/Lon antibody (ab224316), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-LONP1/Lon antibody (ab224316)

    PFA-fixed/Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for LONP1/Lon (green) using ab224316 (4 μg/ml) in ICC/IF.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody (ab224316), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody (ab224316)

    Paraffin embedded human fallopian tissue stained for LONP1/Lon with ab224316 (1/500 dilution) in immunohistochemical analysis.

  • Western blot - Anti-LONP1/Lon antibody (ab224316), expandable thumbnail

    Western blot - Anti-LONP1/Lon antibody (ab224316)

    All lanes: Western blot - Anti-LONP1/Lon antibody (AB224316) at 1/100 dilution

    Lane 1: NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

    Lane 2: NBT-II cell lysate

    Lane 3: PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate

    Predicted band size: 106 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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