AB224316

Anti-LONP1/Lon antibody

Name: Anti-LONP1/Lon antibody (ab224316) | Abcam
Brand: Abcam Limited
SKU: AB224316
Rating: 5 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal LONP1/Lon antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LONP1 aa 350-550.

View Alternative Names

PRSS15, LONP1, LONHs, Lon protease-like protein, Mitochondrial ATP-dependent protease Lon, Serine protease 15, LONP

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-LONP1/Lon antibody (AB224316)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LONP1/Lon antibody (AB224316)

PFA-fixed/Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for LONP1/Lon (green) using ab224316 (4 μg/ml) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody (AB224316)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody (AB224316)

Paraffin embedded human fallopian tissue stained for LONP1/Lon with ab224316 (1/500 dilution) in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody (AB224316)

Paraffin embedded human duodenum tissue stained for LONP1/Lon with ab224316 (1/500 dilution) in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody (AB224316)

Paraffin embedded human tonsil tissue stained for LONP1/Lon with ab224316 (1/500 dilution) in immunohistochemical analysis.

Supplier Data

Western blot - Anti-LONP1/Lon antibody (AB224316)

All lanes:

Western blot - Anti-LONP1/Lon antibody (ab224316) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate

Predicted band size: 106 kDa

false

Supplier Data

Western blot - Anti-LONP1/Lon antibody (AB224316)

All lanes:

Western blot - Anti-LONP1/Lon antibody (ab224316) at 1/100 dilution

All lanes:

SK-MEL-30 cell lysate

Predicted band size: 106 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human, Mouse

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human LONP1 aa 350-550. The exact immunogen used to generate this antibody is proprietary information.

P36776

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix (PubMed : 12198491, PubMed : 15870080, PubMed : 17579211, PubMed : 37327776, PubMed : 8248235). Endogenous substrates include mitochondrial steroidogenic acute regulatory (StAR) protein, DELE1, helicase Twinkle (TWNK) and the large ribosomal subunit protein MRPL32/bL32m (PubMed : 17579211, PubMed : 28377575, PubMed : 37327776). MRPL32/bL32m is protected from degradation by LONP1 when it is bound to a nucleic acid (RNA), but TWNK is not (PubMed : 17579211, PubMed : 28377575). May also have a chaperone function in the assembly of inner membrane protein complexes (By similarity). Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome (PubMed : 17420247). Binds to mitochondrial promoters and RNA in a single-stranded, site-specific, and strand-specific manner (PubMed : 17420247). May regulate mitochondrial DNA replication and/or gene expression using site-specific, single-stranded DNA binding to target the degradation of regulatory proteins binding to adjacent sites in mitochondrial promoters (PubMed : 14739292, PubMed : 17420247).

See full target information LONP1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Renal failure 46:2431150 PubMed39566913

2024

TRPC6 knockdown-mediated ERK1/2 inactivation alleviates podocyte injury in minimal change disease via upregulating Lon peptidase 1.

Applications

Unspecified application

Species

Unspecified reactive species

Jianwei Ma,Liling Ren,Qin Su,Xiuyi Lv,Min Sun,Yunbo Wei,Lili Dai,Xueyan Bian

Scientific reports 14:12314 PubMed38811617

2024

A novel strategy to facilitate uniform epithelial cell maturation using liquid-liquid interfaces.

Applications

Unspecified application

Species

Unspecified reactive species

Rie Sonoi,Masamichi Kamihira

Life (Basel, Switzerland) 13: PubMed37240849

2023

Aerobic Exercise Delays Alzheimer's Disease by Regulating Mitochondrial Proteostasis in the Cerebral Cortex and Hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiyin Cui,Chaoyang Li,Guoliang Fang

Cancers 13: PubMed33671345

2021

The Mitochondrial Protease LonP1 Promotes Proteasome Inhibitor Resistance in Multiple Myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Laure Maneix,Melanie A Sweeney,Sukyeong Lee,Polina Iakova,Shannon E Moree,Ergun Sahin,Premal Lulla,Sarvari V Yellapragada,Francis T F Tsai,Andre Catic

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LONP1/Lon antibody

Immunocytochemistry/ Immunofluorescence - Anti-LONP1/Lon antibody (AB224316)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody (AB224316)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody (AB224316)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody (AB224316)

Western blot - Anti-LONP1/Lon antibody (AB224316)

Western blot - Anti-LONP1/Lon antibody (AB224316)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

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