Anti-LONP1/Lon antibody [EPR28510-62]

12 product images

• 

  Western blot - Anti-LONP1/Lon antibody [EPR28510-62] (ab317561)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-LONP1/Lon antibody [EPR28510-62] (ab317561) at 1/1000 dilution

  Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

  Lane 2: HeLa transfected with siRNA specifically targeting LONP1/Lon whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 106 kDa, 36 kDa

  Exposure time: 10s

• 

  Western blot - Anti-LONP1/Lon antibody [EPR28510-62] (ab317561)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Low expression: thymus (PMID: 8119403).

  Lanes 1-7 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 and lane 8-9 is applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

  Bands observed below the major band at 106kDa represent the isoforms of LONP1/Lon and can be knocked down using siRNA.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  Exposure time: Lanes 1-8 : 10 seconds; Lane 9: 3 seconds

  All lanes: Western blot - Anti-LONP1/Lon antibody [EPR28510-62] (ab317561) at 1/1000 dilution

  Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

  Lane 2: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

  Lane 3: Rat liver tissue lysate at 20 µg

  Lane 4: Rat thymus tissue lysate at 20 µg

  Lane 5: C2C12 (mouse myoblast) at 20 µg

  Lane 6: Mouse liver tissue lysate at 20 µg

  Lane 7: Mouse thymus tissue lysate at 20 µg

  Lane 8: Human liver tissue lysate at 20 µg

  Lane 9: human placenta tissue lysate at 20 µg

  Secondary

  Lanes 1 - 9: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Lanes 1 - 9: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Observed band size: 106 kDa, 36 kDa

• 

  Western blot - Anti-LONP1/Lon antibody [EPR28510-62] (ab317561)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

  Bands observed below the major band at 106kDa represent the isoforms of LONP1/Lon and can be knocked down using siRNA

  All lanes: Western blot - Anti-LONP1/Lon antibody [EPR28510-62] (ab317561) at 1/1000 dilution

  Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

  Lane 2: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

  Lane 3: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

  Lane 4: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

  Lane 5: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 106 kDa

  Exposure time: 15s

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] (ab317561)

  Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling LONP1/Lon with ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on rat colon. The section was incubated with ab317561 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] (ab317561)

  Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling LONP1/Lon with ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on rat lung. The section was incubated with ab317561 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] (ab317561)

  Immunohistochemical analysis of paraffin-embedded Mouse lung cancer tissue labeling LONP1/Lon with ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on mouse lung cancer. The section was incubated with ab317561 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] (ab317561)

  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling LONP1/Lon with ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on mouse colon. The section was incubated with ab317561 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] (ab317561)

  Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling LONP1/Lon with ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on mouse lung. The section was incubated with ab317561 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] (ab317561)

  Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling LONP1/Lon with ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on human colon carcinoma. The section was incubated with ab317561 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] (ab317561)

  Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling LONP1/Lon with ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on human colon. The section was incubated with ab317561 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] (ab317561)

  Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling LONP1/Lon with ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on human lung. The section was incubated with ab317561 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunoprecipitation - Anti-LONP1/Lon antibody [EPR28510-62] (ab317561)

  LONP1/Lon was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab317561 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317561 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

  Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
  
  Lane 2: ab317561 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
  
  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab317561 in HeLa whole cell lysate

  Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  Bands observed below the major band at 106kDa in lane2 represent the isoforms of LONP1/Lon.

  All lanes: Immunoprecipitation - Anti-LONP1/Lon antibody [EPR28510-62] (ab317561) at 1/30 dilution

  All lanes: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Exposure time: 3s