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Rabbit Recombinant Monoclonal LONP1/Lon antibody. Carrier free. Suitable for WB, IP, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (AB317562), expandable thumbnail
  • Western blot - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (AB317562), expandable thumbnail
  • Western blot - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (AB317562), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (AB317562), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (AB317562), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PFlow Cyt (Intra)ICC/IF
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Tested
Expected
Tested
Not recommended
Not recommended
Rat
Tested
Expected
Tested
Not recommended
Not recommended

Tested
Tested

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix (PubMed:12198491, PubMed:15870080, PubMed:17579211, PubMed:37327776, PubMed:8248235). Endogenous substrates include mitochondrial steroidogenic acute regulatory (StAR) protein, DELE1, helicase Twinkle (TWNK) and the large ribosomal subunit protein MRPL32/bL32m (PubMed:17579211, PubMed:28377575, PubMed:37327776). MRPL32/bL32m is protected from degradation by LONP1 when it is bound to a nucleic acid (RNA), but TWNK is not (PubMed:17579211, PubMed:28377575). May also have a chaperone function in the assembly of inner membrane protein complexes (By similarity). Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome (PubMed:17420247). Binds to mitochondrial promoters and RNA in a single-stranded, site-specific, and strand-specific manner (PubMed:17420247). May regulate mitochondrial DNA replication and/or gene expression using site-specific, single-stranded DNA binding to target the degradation of regulatory proteins binding to adjacent sites in mitochondrial promoters (PubMed:14739292, PubMed:17420247).

Alternative names

PRSS15, PRSS15, LONP1, LONHs, Lon protease-like protein, Mitochondrial ATP-dependent protease Lon, Serine protease 15, LONP

Recommended products

Rabbit Recombinant Monoclonal LONP1/Lon antibody. Carrier free. Suitable for WB, IP, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR28510-62

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab317562 is the carrirer-free version of Anti-LONP1/Lon antibody [EPR28510-62] ab317561.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

  • Western blot - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562), expandable thumbnail

    Western blot - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

    This data was developed using Anti-LONP1/Lon antibody [EPR28510-62] ab317561, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-LONP1/Lon antibody [EPR28510-62] (Anti-LONP1/Lon antibody [EPR28510-62] ab317561) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HeLa transfected with siRNA specifically targeting LONP1/Lon whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 106 kDa, 36 kDa

    Exposure time: 10s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  • Western blot - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562), expandable thumbnail

    Western blot - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

    This data was developed using Anti-LONP1/Lon antibody [EPR28510-62] ab317561, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: thymus (PMID: 8119403).

    Lanes 1-7 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 and lane 8-9 is applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

    Bands observed below the major band at 106kDa represent the isoforms of LONP1/Lon and can be knocked down using siRNA.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-8 : 10 seconds; Lane 9: 3 seconds

    All lanes: Western blot - Anti-LONP1/Lon antibody [EPR28510-62] (Anti-LONP1/Lon antibody [EPR28510-62] ab317561) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 2: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

    Lane 3: Rat liver tissue lysate at 20 µg

    Lane 4: Rat thymus tissue lysate at 20 µg

    Lane 5: C2C12 (mouse myoblast) at 20 µg

    Lane 6: Mouse liver tissue lysate at 20 µg

    Lane 7: Mouse thymus tissue lysate at 20 µg

    Lane 8: Human liver tissue lysate at 20 µg

    Lane 9: human placenta tissue lysate at 20 µg

    Secondary

    Lanes 1 - 9: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lanes 1 - 9: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 106 kDa, 36 kDa

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: thymus (PMID: 8119403).

    Lanes 1-7 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 and lane 8-9 is applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

    Bands observed below the major band at 106kDa represent the isoforms of LONP1/Lon and can be knocked down using siRNA.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-8 : 10 seconds; Lane 9: 3 seconds

  • Western blot - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562), expandable thumbnail

    Western blot - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

    This data was developed using Anti-LONP1/Lon antibody [EPR28510-62] ab317561, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    Bands observed below the major band at 106kDa represent the isoforms of LONP1/Lon and can be knocked down using siRNA

    All lanes: Western blot - Anti-LONP1/Lon antibody [EPR28510-62] (Anti-LONP1/Lon antibody [EPR28510-62] ab317561) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 2: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

    Lane 3: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 4: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 106 kDa

    Exposure time: 15s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    Bands observed below the major band at 106kDa represent the isoforms of LONP1/Lon and can be knocked down using siRNA

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

    This data was developed using Anti-LONP1/Lon antibody [EPR28510-62] ab317561, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling LONP1/Lon with Anti-LONP1/Lon antibody [EPR28510-62] ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat colon. The section was incubated with Anti-LONP1/Lon antibody [EPR28510-62] ab317561 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

    This data was developed using Anti-LONP1/Lon antibody [EPR28510-62] ab317561, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling LONP1/Lon with Anti-LONP1/Lon antibody [EPR28510-62] ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat lung. The section was incubated with Anti-LONP1/Lon antibody [EPR28510-62] ab317561 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

    This data was developed using Anti-LONP1/Lon antibody [EPR28510-62] ab317561, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse lung cancer tissue labeling LONP1/Lon with Anti-LONP1/Lon antibody [EPR28510-62] ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse lung cancer. The section was incubated with Anti-LONP1/Lon antibody [EPR28510-62] ab317561 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

    This data was developed using Anti-LONP1/Lon antibody [EPR28510-62] ab317561, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling LONP1/Lon with Anti-LONP1/Lon antibody [EPR28510-62] ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse colon. The section was incubated with Anti-LONP1/Lon antibody [EPR28510-62] ab317561 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

    This data was developed using Anti-LONP1/Lon antibody [EPR28510-62] ab317561, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling LONP1/Lon with Anti-LONP1/Lon antibody [EPR28510-62] ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse lung. The section was incubated with Anti-LONP1/Lon antibody [EPR28510-62] ab317561 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

    This data was developed using Anti-LONP1/Lon antibody [EPR28510-62] ab317561, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling LONP1/Lon with Anti-LONP1/Lon antibody [EPR28510-62] ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human colon carcinoma. The section was incubated with Anti-LONP1/Lon antibody [EPR28510-62] ab317561 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

    This data was developed using Anti-LONP1/Lon antibody [EPR28510-62] ab317561, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling LONP1/Lon with Anti-LONP1/Lon antibody [EPR28510-62] ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human colon. The section was incubated with Anti-LONP1/Lon antibody [EPR28510-62] ab317561 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

    This data was developed using Anti-LONP1/Lon antibody [EPR28510-62] ab317561, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling LONP1/Lon with Anti-LONP1/Lon antibody [EPR28510-62] ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human lung. The section was incubated with Anti-LONP1/Lon antibody [EPR28510-62] ab317561 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunoprecipitation - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562), expandable thumbnail

    Immunoprecipitation - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

    This data was developed using Anti-LONP1/Lon antibody [EPR28510-62] ab317561, the same antibody clone in a different buffer formulation.

    LONP1/Lon was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with Anti-LONP1/Lon antibody [EPR28510-62] ab317561 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-LONP1/Lon antibody [EPR28510-62] ab317561 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 2: Anti-LONP1/Lon antibody [EPR28510-62] ab317561 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-LONP1/Lon antibody [EPR28510-62] ab317561 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Bands observed below the major band at 106kDa in lane2 represent the isoforms of LONP1/Lon.

    All lanes: Immunoprecipitation - Anti-LONP1/Lon antibody [EPR28510-62] (Anti-LONP1/Lon antibody [EPR28510-62] ab317561) at 1/30 dilution

    All lanes: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 3s

    LONP1/Lon was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with Anti-LONP1/Lon antibody [EPR28510-62] ab317561 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-LONP1/Lon antibody [EPR28510-62] ab317561 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 2: Anti-LONP1/Lon antibody [EPR28510-62] ab317561 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-LONP1/Lon antibody [EPR28510-62] ab317561 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Bands observed below the major band at 106kDa in lane2 represent the isoforms of LONP1/Lon.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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