Anti-Loricrin antibody [EPR28296-23] - BSA and Azide free

10 product images

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  Multiplex immunohistochemistry - Anti-Loricrin antibody [EPR28296-23] - BSA and Azide free (ab315807)

  This data was developed using Anti-Loricrin antibody [EPR28296-23] ab315806, the same antibody clone in a different buffer formulation.

  
  Multiplex immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections of mouse skin tissue staining with Anti-Collagen VII antibody [EPR27012-157] ab309143 at 1/100 dilution, Anti-Langerin antibody [EPR28089-89] ab317813 at 1/500 dilution, and Anti-Loricrin antibody [EPR28296-23] ab315806 at 1/500 dilution. The secondary used was Opal Polymer HRP Ms + Rb. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
  Panel A: merged staining of anti-Collagen VII (green; Opal™520), anti-Langerin (gray; Opal™570) and anti-Loricrin (magenta; Opal™690) on mouse skin.
  Panel B: anti-Collagen VII staining the dermal–epidermal junction in mouse skin.
  Panel C: anti-Langerin staining Langerhans cells in mouse skin.
  Panel D: anti-Loricrin staining terminally differentiated epidermal cells in mouse skin.
  Nuclear DNA was labeled with DAPI (shown in blue).
  The section was incubated in three rounds of staining: in the order of Anti-Collagen VII antibody [EPR27012-157] ab309143, Anti-Langerin antibody [EPR28089-89] ab317813 and Anti-Loricrin antibody [EPR28296-23] ab315806 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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  Immunohistochemistry (Frozen sections) - Anti-Loricrin antibody [EPR28296-23] - BSA and Azide free (ab315807)

  This data was developed using Anti-Loricrin antibody [EPR28296-23] ab315806, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skin (fresh frozen) tissue labeling Loricrin with Anti-Loricrin antibody [EPR28296-23] ab315806 at 1/100 (5.22 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

  Confocal image showing positive staining on mouse skin. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Loricrin antibody [EPR28296-23] ab315806 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

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  Immunohistochemistry (Frozen sections) - Anti-Loricrin antibody [EPR28296-23] - BSA and Azide free (ab315807)

  This data was developed using Anti-Loricrin antibody [EPR28296-23] ab315806, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh frozen) tissue labeling Loricrin with Anti-Loricrin antibody [EPR28296-23] ab315806 at 1/100 (5.22 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

  Negative control: confocal image showing no staining on mouse kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Loricrin antibody [EPR28296-23] ab315806 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR28296-23] - BSA and Azide free (ab315807)

  This data was developed using Anti-Loricrin antibody [EPR28296-23] ab315806, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse Loricrin expression vector containing a His tag. (B) HEK-293T transfected with empty vector containing a His tag. tissue labeling Loricrin with Anti-Loricrin antibody [EPR28296-23] ab315806 at 1/500 (1.044 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on HEK-293T transfected with a His-tagged mouse Loricrin construct (image A). No staining on HEK-293T transfected with empty plasmid (image B). The section was incubated with Anti-Loricrin antibody [EPR28296-23] ab315806 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR28296-23] - BSA and Azide free (ab315807)

  This data was developed using Anti-Loricrin antibody [EPR28296-23] ab315806, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Loricrin with Anti-Loricrin antibody [EPR28296-23] ab315806 at 1/500 (1.044 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: no staining on rat spleen. The section was incubated with Anti-Loricrin antibody [EPR28296-23] ab315806 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR28296-23] - BSA and Azide free (ab315807)

  This data was developed using Anti-Loricrin antibody [EPR28296-23] ab315806, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Loricrin with Anti-Loricrin antibody [EPR28296-23] ab315806 at 1/500 (1.044 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: no staining on rat cerebrum. The section was incubated with Anti-Loricrin antibody [EPR28296-23] ab315806 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR28296-23] - BSA and Azide free (ab315807)

  This data was developed using Anti-Loricrin antibody [EPR28296-23] ab315806, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Loricrin with Anti-Loricrin antibody [EPR28296-23] ab315806 at 1/500 (1.044 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: no staining on mouse kidney. The section was incubated with Anti-Loricrin antibody [EPR28296-23] ab315806 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR28296-23] - BSA and Azide free (ab315807)

  This data was developed using Anti-Loricrin antibody [EPR28296-23] ab315806, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Loricrin with Anti-Loricrin antibody [EPR28296-23] ab315806 at 1/500 (1.044 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: no staining on mouse cerebrum. The section was incubated with Anti-Loricrin antibody [EPR28296-23] ab315806 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR28296-23] - BSA and Azide free (ab315807)

  This data was developed using Anti-Loricrin antibody [EPR28296-23] ab315806, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Rat skin tissue labeling Loricrin with Anti-Loricrin antibody [EPR28296-23] ab315806 at 1/500 (1.044 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on rat skin (PMID: 23349860). The section was incubated with Anti-Loricrin antibody [EPR28296-23] ab315806 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR28296-23] - BSA and Azide free (ab315807)

  This data was developed using Anti-Loricrin antibody [EPR28296-23] ab315806, the same antibody clone in a different buffer formulation.

  Immunohistochemical analysis of paraffin-embedded Mouse skin tissue labeling Loricrin with Anti-Loricrin antibody [EPR28296-23] ab315806 at 1/500 (1.044 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on mouse skin. The section was incubated with Anti-Loricrin antibody [EPR28296-23] ab315806 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.