Rabbit Recombinant Monoclonal Loricrin antibody. C-terminal. Suitable for ICC/IF, IHC-P and reacts with Human, Rat samples. Cited in 8 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Loricrin antibody [EPR7149(2)] - C-terminal (AB198994), expandable thumbnail](https://content.abcam.com/products/images/loricrin-antibody-epr71492-c-terminal-ab198994--immunocytochemistry-immunofluorescence-img73127.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Loricrin antibody [EPR7149(2)] - C-terminal (AB198994)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - C-terminal (AB198994), expandable thumbnail](https://content.abcam.com/products/images/loricrin-antibody-epr71492-c-terminal-ab198994--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128931.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - C-terminal (AB198994)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - C-terminal (AB198994), expandable thumbnail](https://content.abcam.com/products/images/loricrin-antibody-epr71492-c-terminal-ab198994--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117491.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - C-terminal (AB198994)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - C-terminal (AB198994), expandable thumbnail](https://content.abcam.com/products/images/loricrin-antibody-epr71492-c-terminal-ab198994--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img68375.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - C-terminal (AB198994)")
Publications
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- Science advances 9:eade25142023Engineering edgeless human skin with enhanced biomechanical properties.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlberto Pappalardo,David Alvarez Cespedes,Shuyang Fang,Abigail R Herschman,Eun Young Jeon,Kristin M Myers,Jeffrey W Kysar,Hasan Erbil AbaciPubMed 36706190
- International journal of molecular sciences 23:2022An Inflamed and Infected Reconstructed Human Epidermis to Study Atopic Dermatitis and Skin Care Ingredients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSébastien Cadau,Manon Gault,Nicolas Berthelemy,Chiung-Yueh Hsu,Louis Danoux,Nicolas Pelletier,Dominique Goudounèche,Carole Pons,Corinne Leprince,Valérie André-Frei,Michel Simon,Sabine PainPubMed 36361668
- Experimental dermatology 32:30-402022RNA sequencing reveals the transcriptome profile of the atopic prurigo nodularis with severe itching.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYixin Shao,Yiqi Zhu,Zijing Xiao,Yanyun Shen,Beiying Dai,Hui Tang,Duoqin WangPubMed 36134503
- Stem cells (Dayton, Ohio) 40:435-4452022CDK12 Is Necessary to Promote Epidermal Differentiation Through Transcription Elongation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingting Li,Manisha Tiwari,Yifang Chen,Sudjit Luanpitpong,George L SenPubMed 35325240
- PLoS biology 19:e30013782021Regulation of integrin and extracellular matrix genes by HNRNPL is necessary for epidermal renewal.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingting Li,Yifang Chen,Manisha Tiwari,Varun Bansal,George L SenPubMed 34543262
- Nature communications 12:7842021SPT6 promotes epidermal differentiation and blockade of an intestinal-like phenotype through control of transcriptional elongation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingting Li,Xiaojun Xu,Manisha Tiwari,Yifang Chen,Mackenzie Fuller,Varun Bansal,Pablo Tamayo,Soumita Das,Pradipta Ghosh,George L SenPubMed 33542242
- The Journal of investigative dermatology 140:2077-2081.e52020BRD4 Is Necessary for Differentiation Downstream of Epidermal Lineage-Determining Transcription Factors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJackson Jones et. al.PubMed 32142793
- The Journal of investigative dermatology 140:2081-2084.e42020TEAD1 and TEAD3 Play Redundant Roles in the Regulation of Human Epidermal Proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingting Li et. al.PubMed 32142794
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Expected | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
5 products for Alternative Product
Alternative names
LOR, LRN, LORICRIN, Loricrin
Recommended products
Rabbit Recombinant Monoclonal Loricrin antibody. C-terminal. Suitable for ICC/IF, IHC-P and reacts with Human, Rat samples. Cited in 8 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR7149(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Loricrin antibody [EPR7149(2)] - C-terminal (ab198994), expandable thumbnail](https://content.abcam.com/products/images/loricrin-antibody-epr71492-c-terminal-ab198994--immunocytochemistry-immunofluorescence-img73127.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Loricrin antibody [EPR7149(2)] - C-terminal (ab198994)")
Immunocytochemistry/ Immunofluorescence - Anti-Loricrin antibody [EPR7149(2)] - C-terminal (ab198994)
Immunocytochemistry/ Immunofluorescence analysis of A431 cell line labeling Loricrin with ab198994 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized using 0.1% Triton X-100. Alexa Fluor® 488 Goat anti-Rabbit Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 used as the secondary at a 1/1000 dilution. Counterstained using Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 anti-Tubulin (mouse mAb) at a 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Alexa Fluor® 594 Goat anti-Mouse secondary at a 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - C-terminal (ab198994), expandable thumbnail](https://content.abcam.com/products/images/loricrin-antibody-epr71492-c-terminal-ab198994--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128931.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - C-terminal (ab198994)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - C-terminal (ab198994)
Immunohistochemical analysis of paraffin-embedded human skin tissue sections labeling Loricrin with ab198994 at a 1/500 dilution. Goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - C-terminal (ab198994), expandable thumbnail](https://content.abcam.com/products/images/loricrin-antibody-epr71492-c-terminal-ab198994--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117491.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - C-terminal (ab198994)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - C-terminal (ab198994)
Immunohistochemical analysis of paraffin-embedded rat skin tissue sections labeling Loricrin with ab198994 at a 1/500 dilution. Goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - C-terminal (ab198994), expandable thumbnail](https://content.abcam.com/products/images/loricrin-antibody-epr71492-c-terminal-ab198994--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img68375.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - C-terminal (ab198994)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - C-terminal (ab198994)
Immunohistochemical analysis of paraffin-embedded human uterus tissue sections labeling Loricrin with ab198994 at a 1/500 dilution. Goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin. Used as a negative tissue control.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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