AB214427

Anti-LOX 1 antibody [EPR20750]

RabMAb
Recombinant
20ul selling size
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(11 Publications)

Rabbit Recombinant Monoclonal LOX 1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 11 publications.

View Alternative Names

CLEC8A, LOX1, OLR1, Oxidized low-density lipoprotein receptor 1, Ox-LDL receptor 1, C-type lectin domain family 8 member A, Lectin-like oxidized LDL receptor 1, Lectin-type oxidized LDL receptor 1, LOX-1, Lectin-like oxLDL receptor 1, hLOX-1

2 Images

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Western blot - Anti-LOX 1 antibody [EPR20750] (AB214427)

All lanes:

Western blot - Anti-LOX 1 antibody [EPR20750] (ab214427) at 1/1000 dilution

Lane 1:

Human fetal vessel lysate at 20 µg

Lane 2:

Mouse placenta lysate at 20 µg

Lane 3:

Rat placenta lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 31 kDa

Observed band size: 52 kDa

false

Supplier Data

Western blot - Anti-LOX 1 antibody [EPR20750] (AB214427)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile is consistent with what has been described in the literature (PMID : 10692464, PMID : 15016631).

All lanes:

Western blot - Anti-LOX 1 antibody [EPR20750] (ab214427) at 1/1000 dilution

Lane 1:

Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate at 20 µg

Lane 2:

THP-1 treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 hours, whole cell lysate at 20 µg

Lane 3:

Untreated HUVEC (human umbilical vein endothelial cell line) whole cell lysate at 20 µg

Lane 4:

HUVEC treated with 100 ng/ml phorbol-12-myristate-13-acetate (PMA) for 8 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 31 kDa

Observed band size: 52 kDa

false

Exposure time: 3min

Carrier free

Anti-LOX 1 antibody [EPR20750] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20750

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.. (Microbial infection) May serve as a receptor for adhesin A variant 3 (nadA) of N.meningitidis.

See full target information OLR1

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Genes & diseases 12:101761 PubMed40837406

2025

Tirzepatide, a dual GIP/GLP-1 receptor agonist, alleviates metabolic dysfunction-associated steatotic liver disease by reducing the expression of CD36 and OBP2A.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Li,Wencong Sun,Hong Liu,Xiong Z Ruan

Endokrynologia Polska 76:116-123 PubMed40071808

2025

PCSK9/LOX-1 is associated with T2DM and regulates high glucose-induced lipid metabolism dysfunction in human microvascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Yao,Hong Liu

Food science & nutrition 12:8000-8012 PubMed39479713

2024

The potential of in the treatment of hyperlipidemia and its interaction with the LOX1-PI3K-AKT-eNOS pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuyang Shen,Xin Gao,Dan Huang,Xiaojin Xu,Jianping Shen

Frontiers in neuroscience 18:1442110 PubMed39234183

2024

Lectin-type oxidized LDL receptor-1 as a potential therapeutic target for cerebral cavernous malformations treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Karthik Ashok,Tyra Martinez,Julie Sesen,Sana Nasim,Shih-Shan Lang,Gregory Heuer,Alexander Tucker,Miguel Alejandro Lopez-Ramirez,Edward R Smith,Aram Ghalali

Annals of translational medicine 11:42 PubMed36819529

2023

TRIM59 attenuates ox-LDL-induced endothelial cell inflammation, apoptosis, and monocyte adhesion through AnxA2.

Applications

Unspecified application

Species

Unspecified reactive species

Qilin Zeng,Jingli Xie,Fang Li

Annals of translational medicine 10:700 PubMed35845495

2022

Anti-atherosclerotic effects of genistein in preventing ox-low-density lipoprotein-induced smooth muscle-derived foam cell formation via inhibiting expression and L-Ca channel currents.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Liming Zhang,Xiaosi Zhang

Bioengineered 13:13872-13881 PubMed35707830

2022

Protective roles of apremilast via Sirtuin 1 in atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dongkui Sui,Hua Yu

Molecular medicine reports 25: PubMed35293601

2022

SESN1 attenuates the Ox‑LDL‑induced inflammation, apoptosis and endothelial‑mesenchymal transition of human umbilical vein endothelial cells by regulating AMPK/SIRT1/LOX1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Gao,Yongcheng Zhao,Bin Zhang,Chunwei Xiao,Zhanfa Sun,Yuan Gao,Xueyong Dou

Cell death & disease 13:4 PubMed34921134

2021

Knockdown of OLR1 weakens glycolytic metabolism to repress colon cancer cell proliferation and chemoresistance by downregulating SULT2B1 via c-MYC.

Applications

Unspecified application

Species

Unspecified reactive species

Tiancheng Zhao,Yezhou Li,Kexin Shen,Quan Wang,Jiayu Zhang

BioMed research international 2021:9983725 PubMed34471643

2021

Curcumin Alleviates Palmitic Acid-Induced LOX-1 Upregulation by Suppressing Endoplasmic Reticulum Stress in HUVECs.

Applications

Unspecified application

Species

Unspecified reactive species

Ruixi Luo,Lifeng Zhao,Shuaishuai Li,Peng Chen,La Wang,Honghong Yu,Kun Cai,Qi Yu,Weiyi Tian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LOX 1 antibody [EPR20750]

Western blot - Anti-LOX 1 antibody [EPR20750] (AB214427)

Western blot - Anti-LOX 1 antibody [EPR20750] (AB214427)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (11)

Product promise