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Rabbit Recombinant Monoclonal LOX 1 antibody. Carrier free. Suitable for WB, IHC-P, IP and reacts with Mouse, Rat samples.

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Images

Western blot - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (AB317690), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (AB317690), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (AB317690), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (AB317690), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (AB317690), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIPFlow CytICC/IF
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Not recommended
Not recommended
Rat
Tested
Tested
Expected
Not recommended
Not recommended

Tested
Tested

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal LOX 1 antibody. Carrier free. Suitable for WB, IHC-P, IP and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR27871-67
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab317690 is the carrier-free version of Anti-LOX 1 antibody [EPR27871-67] ab317689.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (ab317690), expandable thumbnail

    Western blot - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (ab317690)

    This data was developed using Anti-LOX 1 antibody [EPR27871-67] ab317689, the same antibody clone in a different buffer formulation.

    Low expression: liver (PMID: 9828121, PMID: 9052782).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 9052782, PMID: 12354387).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-LOX 1 antibody [EPR27871-67] (Anti-LOX 1 antibody [EPR27871-67] ab317689) at 1/1000 dilution

    Lane 1: Rat placenta tissue lysate at 20 µg with NFDM/TBST

    Lane 2: Rat liver tissue lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 30-37 kDa, 36 kDa

    Exposure time: 37s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (ab317690), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (ab317690)

    This data was developed using Anti-LOX 1 antibody [EPR27871-67] ab317689, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling LOX 1 with Anti-LOX 1 antibody [EPR27871-67] ab317689 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: No staining in rat skeletal muscle.

    The section was incubated with Anti-LOX 1 antibody [EPR27871-67] ab317689 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (ab317690), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (ab317690)

    This data was developed using Anti-LOX 1 antibody [EPR27871-67] ab317689, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat placenta tissue labeling LOX 1 with Anti-LOX 1 antibody [EPR27871-67] ab317689 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in trophoblast of rat placenta.

    The section was incubated with Anti-LOX 1 antibody [EPR27871-67] ab317689 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (ab317690), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (ab317690)

    This data was developed using Anti-LOX 1 antibody [EPR27871-67] ab317689, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling LOX 1 with Anti-LOX 1 antibody [EPR27871-67] ab317689 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: No staining in mouse skeletal muscle.

    The section was incubated with Anti-LOX 1 antibody [EPR27871-67] ab317689 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (ab317690), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (ab317690)

    This data was developed using Anti-LOX 1 antibody [EPR27871-67] ab317689, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling LOX 1 with Anti-LOX 1 antibody [EPR27871-67] ab317689 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in immune cell of mouse spleen.

    The section was incubated with Anti-LOX 1 antibody [EPR27871-67] ab317689 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (ab317690), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (ab317690)

    This data was developed using Anti-LOX 1 antibody [EPR27871-67] ab317689, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse placenta tissue labeling LOX 1 with Anti-LOX 1 antibody [EPR27871-67] ab317689 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in trophoblast of mouse placenta (PMID: 18474940).

    The section was incubated with Anti-LOX 1 antibody [EPR27871-67] ab317689 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunoprecipitation - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (ab317690), expandable thumbnail

    Immunoprecipitation - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (ab317690)

    This data was developed using Anti-LOX 1 antibody [EPR27871-67] ab317689, the same antibody clone in a different buffer formulation.

    LOX 1 was immunoprecipitated from 0.35 mg Mouse placenta tissue lysate with Anti-LOX 1 antibody [EPR27871-67] ab317689 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-LOX 1 antibody [EPR27871-67] ab317689 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse placenta tissue lysate

    Lane 2: Anti-LOX 1 antibody [EPR27871-67] ab317689 IP in Mouse placenta tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-LOX 1 antibody [EPR27871-67] ab317689 in mouse placenta tissue lysate

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 9052782, PMID: 12354387).

    All lanes: Immunoprecipitation - Anti-LOX 1 antibody [EPR27871-67] (Anti-LOX 1 antibody [EPR27871-67] ab317689) at 1/30 dilution

    All lanes: Mouse placenta tissue lysate at 10 µg with NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 3s

  • Western blot - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (ab317690), expandable thumbnail

    Western blot - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (ab317690)

    This data was developed using Anti-LOX 1 antibody [EPR27871-67] ab317689, the same antibody clone in a different buffer formulation.

    Low expression: liver, skeletal muscle, brain (PMID: 9828121, PMID: 9052782).

    LOX1 is a glycoprotein of approximately 50-75 kDa and detected as a 30-37 kDa band after treated with Peptide:N-glycosidase F (PNGase F).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 9052782, PMID: 12354387).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-LOX 1 antibody [EPR27871-67] (Anti-LOX 1 antibody [EPR27871-67] ab317689) at 1/1000 dilution

    Lane 1: Mouse placenta tissue lysate at 20 µg with NFDM/TBST

    Lane 2: Mouse liver tissue lysate at 20 µg with NFDM/TBST

    Lane 3: Mouse skeletal muscle tissue lysate at 20 µg with NFDM/TBST

    Lane 4: Mouse brain tissue lysate at 20 µg with NFDM/TBST

    Lane 5: Untreated Mouse placenta tissue lysate, at 20 µg with NFDM/TBST

    Lane 6: Mouse placenta tissue lysate treated with Peptide:N-glycosidase F (PNGase F), at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 30-37 kDa, 50-75 kDa, 36 kDa

    Exposure time: 15s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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