Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (AB317690)

This data was developed using ab317689, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse placenta tissue labeling LOX 1 with ab317689 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in trophoblast of mouse placenta (PMID : 18474940).
The section was incubated with ab317689 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (AB317690)

This data was developed using ab317689, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse placenta tissue staining LOX 1 with ab317689 at a 1/2000 dilution, ab324045 anti-AP2 gamma used at 1/500 dilution and ab320823 anti-Trophoblast specific protein alpha used at a 1/1000 dilution.

Panel A : merged staining of anti-LOX 1 (green; Opal™520), anti-AP2 gamma (magenta; Opal™690) and anti-Trophoblast specific protein alpha (gray; Opal™570) on mouse placenta.

Panel B : anti-LOX 1 staining trophoblast in mouse placenta.

Panel C : ant-AP2 gamma staining trophoblast stem cell in mouse placenta.

Panel D : ant-Trophoblast specific protein alpha staining junctional zone in mouse placenta.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab317689, ab324045 and ab320823 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (AB317690)

This data was developed using ab317689, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat placenta tissue labeling LOX 1 with ab317689 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in trophoblast of rat placenta.
The section was incubated with ab317689 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (AB317690)

This data was developed using ab317689, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling LOX 1 with ab317689 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in immune cell of mouse spleen.
The section was incubated with ab317689 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (AB317690)

This data was developed using ab317689, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling LOX 1 with ab317689 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining in mouse skeletal muscle.
The section was incubated with ab317689 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (AB317690)

This data was developed using ab317689, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling LOX 1 with ab317689 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining in rat skeletal muscle.
The section was incubated with ab317689 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (AB317690)

This data was developed using ab317689, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse placenta tissue staining LOX 1 with ab317689 at a 1/2000 dilution, ab324045 anti-AP2 gamma used at 1/500 dilution and ab182981 anti-CD31 used at a 1/5000 dilution.

Panel A : merged staining of anti-LOX 1 (green; Opal™520), anti-AP2 gamma (magenta; Opal™690) and anti-CD31 (gray; Opal™570) on mouse placenta.

Panel B : anti-LOX 1 staining trophoblast in mouse placenta.

Panel C : ant-AP2 gamma staining trophoblast stem cell in mouse placenta.

Panel D : ant-CD31 staining endothelium in mouse placenta.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab317689, ab324045 and ab182981 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (AB317690)

This data was developed using ab317689, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse placenta tissue staining LOX 1 with ab317689 at a 1/2000 dilution, ab324045 anti-AP2 gamma used at 1/500 dilution and ab182981 anti-CD31 used at a 1/5000 dilution.

Panel A : merged staining of anti-LOX 1 (green; Opal™520), anti-AP2 gamma (magenta; Opal™690) and anti-CD31 (gray; Opal™570) on mouse placenta.

Panel B : anti-LOX 1 staining trophoblast in mouse placenta.

Panel C : ant-AP2 gamma staining trophoblast stem cell in mouse placenta.

Panel D : ant-CD31 staining endothelium in mouse placenta.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab317689, ab324045 and ab182981 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (AB317690)

This data was developed using ab317689, the same antibody clone in a different buffer formulation.

LOX 1 was immunoprecipitated from 0.35 mg Mouse placenta tissue lysate with ab317689 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317689 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse placenta tissue lysate
Lane 2 : ab317689 IP in Mouse placenta tissue lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab317689 in mouse placenta tissue lysate

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 9052782, PMID : 12354387).

All lanes:

Immunoprecipitation - Anti-LOX 1 antibody [EPR27871-67] (<a href='/en-us/products/primary-antibodies/lox-1-antibody-epr27871-67-ab317689'>ab317689</a>) at 1/30 dilution

All lanes:

Mouse placenta tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (AB317690)

This data was developed using ab317689, the same antibody clone in a different buffer formulation.

Low expression : liver, skeletal muscle, brain (PMID : 9828121, PMID : 9052782).

LOX1 is a glycoprotein of approximately 50-75 kDa and detected as a 30-37 kDa band after treated with Peptide : N-glycosidase F (PNGase F).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 9052782, PMID : 12354387).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-LOX 1 antibody [EPR27871-67] (<a href='/en-us/products/primary-antibodies/lox-1-antibody-epr27871-67-ab317689'>ab317689</a>) at 1/1000 dilution

Lane 1:

Mouse placenta tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Untreated Mouse placenta tissue lysate, at 20 µg

Lane 6:

Mouse placenta tissue lysate treated with Peptide:N-glycosidase F (PNGase F), at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30-37 kDa,50-75 kDa,36 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-LOX 1 antibody [EPR27871-67] - BSA and Azide free (AB317690)

This data was developed using ab317689, the same antibody clone in a different buffer formulation.

Low expression : liver (PMID : 9828121, PMID : 9052782).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 9052782, PMID : 12354387).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-LOX 1 antibody [EPR27871-67] (<a href='/en-us/products/primary-antibodies/lox-1-antibody-epr27871-67-ab317689'>ab317689</a>) at 1/1000 dilution

Lane 1:

Rat placenta tissue lysate at 20 µg

Lane 2:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30-37 kDa,36 kDa

false

Exposure time: 37s