AB31238

Anti-LOX antibody

Name: Anti-LOX antibody. Rabbit polyclonal (ab31238) | Abcam
Brand: Abcam Limited
SKU: AB31238
Price: 520 USD
Availability: InStock
Rating: 4 (17 reviews)

(17 Reviews)

(87 Publications)

Rabbit Polyclonal LOX antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 87 publications.

View Alternative Names

Protein-lysine 6-oxidase, Lysyl oxidase, LOX

2 Images

Western blot - Anti-LOX antibody (AB31238)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab31238 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-LOX antibody (ab31238) at 1 µg/mL

Lane 1:

MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

NIH 3T3 (Mouse) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

Predicted band size: 47 kDa

Observed band size: 15 kDa,36 kDa,47 kDa,52 kDa

true

Exposure time: 4min

Supplier Data

Western blot - Anti-LOX antibody (AB31238)

False colour image of Western blot : Anti-LOX antibody staining at 1 ug/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab31238 was shown to bind specifically to LOX. A band was observed at 50 kDa in wild-type HeLa cell lysates with no signal observed at this size in Lox knockout cell line ab261801 (knockout cell lysate ab256981). To generate this image, wild-type and Lox knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-LOX antibody (ab31238) at 1 µg/mL

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Wild-type HeLa Treated DFO (0.5 mM, 24 h) cell lysate at 20 µg

Lane 2:

Western blot - Human LOX knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-lox-knockout-hela-cell-line-ab261801'>ab261801</a>)

Lane 3:

LOX knockout HeLa Vehicle Control DFO (0 mM, 24 h) cell lysate at 20 µg

Lane 4:

LOX knockout HeLa Treated DFO (0.5 mM, 24 h) cell lysate at 20 µg

Predicted band size: 47 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p>Abcam recommends using Milk as the blocking agent.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p>Abcam recommends using Milk as the blocking agent.</p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin (PubMed : 26838787). Regulator of Ras expression. May play a role in tumor suppression. Plays a role in the aortic wall architecture (By similarity).

See full target information Protein-lysine 6-oxidase

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Publications (87)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1736 PubMed39966385

2025

A glucose-enriched lung pre-metastatic niche triggered by matrix stiffness-tuned exosomal miRNAs in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Zhao,Hongmei Yu,Jiajun Li,Jiali Qian,Miao Li,Xi Zhang,Mimi Wang,Yaohui Wang,Yinying Dong,Yang You,Qiwen Zhou,Dongmei Gao,Yan Zhao,Binbin Liu,Rongxin Chen,Zhenggang Ren,Zhiming Wang,Kezhi Zhang,Jiefeng Cui

Journal of translational medicine 23:35 PubMed39789539

2025

LOX-induced tubulointerstitial fibrosis via the TGF-β/LOX/Snail axis in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yicheng Lu,Heyangzi Li,Mohan Chen,Yicheng Lin,Xiaoming Zhang

Redox biology 76:103346 PubMed39260063

2024

LOX-mediated ECM mechanical stress induces Piezo1 activation in hypoxic-ischemic brain damage and identification of novel inhibitor of LOX.

Applications

Unspecified application

Species

Unspecified reactive species

Dongya Jiang,Jing Zhao,Jie Zheng,Yingmin Zhao,Meini Le,Dani Qin,Qiong Huang,Jinyu Huang,Qingshun Zhao,Long Wang,Xiaohua Dong

Antioxidants (Basel, Switzerland) 13: PubMed38790628

2024

Lysyl Oxidase in Ectopic Cardiovascular Calcification: Role of Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Carme Ballester-Servera,Judith Alonso,Laia Cañes,Paula Vázquez-Sufuentes,Ana B García-Redondo,Cristina Rodríguez,José Martínez-González

Nutrients 16: PubMed38337716

2024

Moderate-Intensity and High-Intensity Interval Exercise Training Offer Equal Cardioprotection, with Different Mechanisms, during the Development of Type 2 Diabetes in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah D'Haese,Lisa Claes,Iris de Laat,Sven Van Campenhout,Dorien Deluyker,Ellen Heeren,Sibren Haesen,Ivo Lambrichts,Kristiaan Wouters,Casper G Schalkwijk,Dominique Hansen,B O Eijnde,Virginie Bito

Nutrients 15: PubMed37764732

2023

Moderate- and High-Intensity Endurance Training Alleviate Diabetes-Induced Cardiac Dysfunction in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah D'Haese,Maxim Verboven,Lize Evens,Dorien Deluyker,Ivo Lambrichts,B O Eijnde,Dominique Hansen,Virginie Bito

Journal of advanced research 54:223-237 PubMed36706988

2023

Single-cell analysis reveals lysyl oxidase (Lox) fibroblast subset involved in cardiac fibrosis of diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Heyangzi Li,Xiaoqing Zhu,Xi Cao,Yicheng Lu,Jianwei Zhou,Xiaoming Zhang

Tissue engineering. Part A 29:225-243 PubMed36597287

2023

Sodium Nitroprusside Stimulation of Elastic Matrix Regeneration by Aneurysmal Smooth Muscle Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Suraj Bastola,Chandrasekhar Kothapalli,Anand Ramamurthi

American journal of human genetics 109:2230-2252 PubMed36351433

2022

EMILIN1 deficiency causes arterial tortuosity with osteopenia and connects impaired elastogenesis with defective collagen fibrillogenesis.

Applications

WB, ICC/IF

Species

Mouse, Mouse

Christin S Adamo,Aude Beyens,Alvise Schiavinato,Douglas R Keene,Sara F Tufa,Matthias Mörgelin,Jürgen Brinckmann,Takako Sasaki,Anja Niehoff,Maren Dreiner,Lore Pottie,Laura Muiño-Mosquera,Elif Yilmaz Gulec,Alper Gezdirici,Paola Braghetta,Paolo Bonaldo,Raimund Wagener,Mats Paulsson,Helen Bornaun,Riet De Rycke,Michiel De Bruyne,Femke Baeke,Walter P Devine,Balram Gangaram,Allison Tam,Meena Balasubramanian,Sian Ellard,Sandra Moore,Sofie Symoens,Joseph Shen,Stacey Cole,Ulrike Schwarze,Kathryn W Holmes,Susan J Hayflick,Wojciech Wiszniewski,Sheela Nampoothiri,Elaine C Davis,Lynn Y Sakai,Gerhard Sengle,Bert Callewaert

International journal of molecular sciences 23: PubMed36232621

2022

Knock-Out of the Five Lysyl-Oxidase Family Genes Enables Identification of Lysyl-Oxidase Pro-Enzyme Regulated Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Tatyana Liburkin-Dan,Inbal Nir-Zvi,Hila Razon,Ofra Kessler,Gera Neufeld

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LOX antibody

Western blot - Anti-LOX antibody (AB31238)

Western blot - Anti-LOX antibody (AB31238)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (87)

Product promise