Rabbit Polyclonal LOX antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 77 publications.
Images
Publications
Filter by
- Nutrients 15:2023Moderate- and High-Intensity Endurance Training Alleviate Diabetes-Induced Cardiac Dysfunction in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah D'Haese,Maxim Verboven,Lize Evens,Dorien Deluyker,Ivo Lambrichts,B O Eijnde,Dominique Hansen,Virginie BitoPubMed 37764732
- American journal of human genetics 109:2230-22522022EMILIN1 deficiency causes arterial tortuosity with osteopenia and connects impaired elastogenesis with defective collagen fibrillogenesis.Applications:WB, ICC/IFReactive species:MouseChristin S Adamo,Aude Beyens,Alvise Schiavinato,Douglas R Keene,Sara F Tufa,Matthias Mörgelin,Jürgen Brinckmann,Takako Sasaki,Anja Niehoff,Maren Dreiner,Lore Pottie,Laura Muiño-Mosquera,Elif Yilmaz Gulec,Alper Gezdirici,Paola Braghetta,Paolo Bonaldo,Raimund Wagener,Mats Paulsson,Helen Bornaun,Riet De Rycke,Michiel De Bruyne,Femke Baeke,Walter P Devine,Balram Gangaram,Allison Tam,Meena Balasubramanian,Sian Ellard,Sandra Moore,Sofie Symoens,Joseph Shen,Stacey Cole,Ulrike Schwarze,Kathryn W Holmes,Susan J Hayflick,Wojciech Wiszniewski,Sheela Nampoothiri,Elaine C Davis,Lynn Y Sakai,Gerhard Sengle,Bert CallewaertPubMed 36351433
- International journal of molecular sciences 23:2022Knock-Out of the Five Lysyl-Oxidase Family Genes Enables Identification of Lysyl-Oxidase Pro-Enzyme Regulated Genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTatyana Liburkin-Dan,Inbal Nir-Zvi,Hila Razon,Ofra Kessler,Gera NeufeldPubMed 36232621
- Nature communications 13:55552022Topical application of an irreversible small molecule inhibitor of lysyl oxidases ameliorates skin scarring and fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNutan Chaudhari,Alison D Findlay,Andrew W Stevenson,Tristan D Clemons,Yimin Yao,Amar Joshi,Sepidar Sayyar,Gordon Wallace,Suzanne Rea,Priyanka Toshniwal,Zhenjun Deng,Philip E Melton,Nicole Hortin,K Swaminathan Iyer,Wolfgang Jarolimek,Fiona M Wood,Mark W FearPubMed 36138009
- Frontiers in cell and developmental biology 10:9441262022Craniofacial tendon development-Characterization of extracellular matrix morphology and spatiotemporal protein distribution.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefanie H Korntner,Aniket Jana,Elizabeth Kinnard,Emily Leo,Timothy Beane,Xianmu Li,Rohit Sengupta,Lauren Becker,Catherine K KuoPubMed 36158210
- International journal of molecular sciences 23:2022Correlation of Matrisome-Associatted Gene Expressions with LOX Family Members in Astrocytomas Stratified by IDH Mutation Status.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTalita de Sousa Laurentino,Roseli da Silva Soares,Suely Kazue Nagahashi Marie,Sueli Mieko Oba-ShinjoPubMed 36076905
- International journal of molecular sciences 23:2022Pan-Lysyl Oxidase Inhibitor PXS-5505 Ameliorates Multiple-Organ Fibrosis by Inhibiting Collagen Crosslinks in Rodent Models of Systemic Sclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYimin Yao,Alison Findlay,Jessica Stolp,Benjamin Rayner,Kjetil Ask,Wolfgang JarolimekPubMed 35628342
- Theranostics 12:910-9282022Dysregulation of interaction between LOX fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYinan Chen,Tao Zhang,Fang Yao,Xiang Gao,Dandan Li,Shufang Fu,Lin Mao,Fei Liu,Xuelin Zhang,Yongle Xu,Jianqing Deng,Weihao Li,Guangpu Fan,Cangsong Xiao,Yu Chen,Li Wang,Wei Guo,Bingying ZhouPubMed 34976220
- Antioxidants (Basel, Switzerland) 11:2021Human Lysyl Oxidase Over-Expression Enhances Baseline Cardiac Oxidative Stress but Does Not Aggravate ROS Generation or Infarct Size Following Myocardial Ischemia-Reperfusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Valls-Lacalle,Lídia Puertas-Umbert,Saray Varona,José Martínez-González,Cristina Rodríguez,Antonio Rodríguez-SinovasPubMed 35052579
- Reproductive medicine and biology 21:e124232021Higher fibrotic content of endometriotic lesions is associated with diminished prostaglandin E2 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingqing Huang,Xishi Liu,Sun-Wei GuoPubMed 34938147
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Predicted |
| Chicken | Predicted |
| Dog | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1-5 µg/mL | Notes Abcam recommends using Milk as the blocking agent. |
Species Human | Dilution info 1-5 µg/mL | Notes Abcam recommends using Milk as the blocking agent. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Dog | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin (PubMed:26838787). Regulator of Ras expression. May play a role in tumor suppression. Plays a role in the aortic wall architecture (By similarity).
Alternative names
Protein-lysine 6-oxidase, Lysyl oxidase, LOX
Recommended products
Rabbit Polyclonal LOX antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 77 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-LOX antibody (ab31238)
False colour image of Western blot: Anti-LOX antibody staining at 1 ug/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab31238 was shown to bind specifically to LOX. A band was observed at 50 kDa in wild-type HeLa cell lysates with no signal observed at this size in Lox knockout cell line Human LOX knockout HeLa cell line ab261801 (knockout cell lysate ab256981). To generate this image, wild-type and Lox knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-LOX antibody (ab31238) at 1 µg/mL
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: Wild-type HeLa Treated DFO (0.5 mM, 24 h) cell lysate at 20 µg
Lane 2: Western blot - Human LOX knockout HeLa cell line (Human LOX knockout HeLa cell line ab261801)
Lane 3: LOX knockout HeLa Vehicle Control DFO (0 mM, 24 h) cell lysate at 20 µg
Lane 4: LOX knockout HeLa Treated DFO (0.5 mM, 24 h) cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 50 kDa
Western blot - Anti-LOX antibody (ab31238)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab31238 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-LOX antibody (ab31238) at 1 µg/mL
Lane 1: MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: NIH 3T3 (Mouse) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 15 kDa, 36 kDa, 47 kDa, 52 kDa
Exposure time: 4min
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com