Anti-LOXL1 antibody [EPR28297-2] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-LOXL1 antibody [EPR28297-2] - BSA and Azide free (AB313586)

This data was developed using ab313585, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human LYOX. In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. Exposure time : 15 seconds

All lanes:

Western blot - Anti-LOXL1 antibody [EPR28297-2] (<a href='/en-us/products/primary-antibodies/loxl1-antibody-epr28297-2-ab313585'>ab313585</a>) at 1/1000 dilution

Lane 1:

His-tagged human LOXL1 recombinant protein at 20 ng

Lane 2:

293T cells transfected with a human LYOX vector containi a myc-His-tag® whole cell lysate at 800 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 63 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-LOXL1 antibody [EPR28297-2] - BSA and Azide free (AB313586)

This data was developed using ab313585, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : K562, THP-1 The bands beneath the target band (63 kDa) are likely to be degraded target fragments. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 48 seconds

All lanes:

Western blot - Anti-LOXL1 antibody [EPR28297-2] (<a href='/en-us/products/primary-antibodies/loxl1-antibody-epr28297-2-ab313585'>ab313585</a>) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 40 µg

Lane 2:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 3:

K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 40 µg

Lane 4:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 63 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-LOXL1 antibody [EPR28297-2] - BSA and Azide free (AB313586)

This data was developed using ab313585, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST The bands beneath the target band (63 kDa) are likely to be degraded target fragments. Exposure time : 59 seconds

All lanes:

Western blot - Anti-LOXL1 antibody [EPR28297-2] (<a href='/en-us/products/primary-antibodies/loxl1-antibody-epr28297-2-ab313585'>ab313585</a>) at 1/1000 dilution

All lanes:

Mouse lung tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 63 kDa

false

Exposure time: 59s