Rabbit Recombinant Monoclonal LOXL1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Recombinant fragment - Human samples.
Images
![Western blot - Anti-LOXL1 antibody [EPR28297-2] - BSA and Azide free (AB313586), expandable thumbnail](https://content.abcam.com/products/images/loxl1-antibody-epr28297-2-bsa-and-azide-free-ab313586--western-blot-img418213.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LOXL1 antibody [EPR28297-2] - BSA and Azide free (AB313586)")
![Western blot - Anti-LOXL1 antibody [EPR28297-2] - BSA and Azide free (AB313586), expandable thumbnail](https://content.abcam.com/products/images/loxl1-antibody-epr28297-2-bsa-and-azide-free-ab313586--western-blot-img418211.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LOXL1 antibody [EPR28297-2] - BSA and Azide free (AB313586)")
![Western blot - Anti-LOXL1 antibody [EPR28297-2] - BSA and Azide free (AB313586), expandable thumbnail](https://content.abcam.com/products/images/loxl1-antibody-epr28297-2-bsa-and-azide-free-ab313586--western-blot-img418212.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LOXL1 antibody [EPR28297-2] - BSA and Azide free (AB313586)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Recombinant fragment - Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Catalyzes the oxidative deamination of lysine and hydroxylysine residues in collagen and elastin, resulting in the formation of covalent cross-linkages, and the stabilization of collagen and elastin fibers (By similarity). Essential for the elastic fiber homeostasis and for their maintenance at adult age (By similarity).
Alternative names
LOXL, LOXL1, Lysyl oxidase homolog 1, Lysyl oxidase-like protein 1, LOL
Recommended products
Rabbit Recombinant Monoclonal LOXL1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Recombinant fragment - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28297-2
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-LOXL1 antibody [EPR28297-2] - BSA and Azide free (ab313586), expandable thumbnail](https://content.abcam.com/products/images/loxl1-antibody-epr28297-2-bsa-and-azide-free-ab313586--western-blot-img418213.jpg "Western blot - Anti-LOXL1 antibody [EPR28297-2] - BSA and Azide free (ab313586)")
Western blot - Anti-LOXL1 antibody [EPR28297-2] - BSA and Azide free (ab313586)
This data was developed using Anti-LOXL1 antibody [EPR28297-2] ab313585, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with human LYOX.
In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
Exposure time: 15 secondsAll lanes: Western blot - Anti-LOXL1 antibody [EPR28297-2] (Anti-LOXL1 antibody [EPR28297-2] ab313585) at 1/1000 dilution
Lane 1: His-tagged human LOXL1 recombinant protein at 20 ng
Lane 2: 293T cells transfected with a human LYOX vector containi a myc-His-tag® whole cell lysate at 800 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 63 kDa
Exposure time: 15s
![Western blot - Anti-LOXL1 antibody [EPR28297-2] - BSA and Azide free (ab313586), expandable thumbnail](https://content.abcam.com/products/images/loxl1-antibody-epr28297-2-bsa-and-azide-free-ab313586--western-blot-img418211.jpg "Western blot - Anti-LOXL1 antibody [EPR28297-2] - BSA and Azide free (ab313586)")
Western blot - Anti-LOXL1 antibody [EPR28297-2] - BSA and Azide free (ab313586)
This data was developed using Anti-LOXL1 antibody [EPR28297-2] ab313585, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: K562, THP-1
The bands beneath the target band (63 kDa) are likely to be degraded target fragments.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 48 secondsAll lanes: Western blot - Anti-LOXL1 antibody [EPR28297-2] (Anti-LOXL1 antibody [EPR28297-2] ab313585) at 1/1000 dilution
Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 40 µg
Lane 2: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 40 µg
Lane 3: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 40 µg
Lane 4: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 63 kDa
Exposure time: 48s
![Western blot - Anti-LOXL1 antibody [EPR28297-2] - BSA and Azide free (ab313586), expandable thumbnail](https://content.abcam.com/products/images/loxl1-antibody-epr28297-2-bsa-and-azide-free-ab313586--western-blot-img418212.jpg "Western blot - Anti-LOXL1 antibody [EPR28297-2] - BSA and Azide free (ab313586)")
Western blot - Anti-LOXL1 antibody [EPR28297-2] - BSA and Azide free (ab313586)
This data was developed using Anti-LOXL1 antibody [EPR28297-2] ab313585, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The bands beneath the target band (63 kDa) are likely to be degraded target fragments.
Exposure time: 59 secondsAll lanes: Western blot - Anti-LOXL1 antibody [EPR28297-2] (Anti-LOXL1 antibody [EPR28297-2] ab313585) at 1/1000 dilution
All lanes: Mouse lung tissue lysate at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 63 kDa
Exposure time: 59s
Downloads
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