Anti-LOXL2 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LOXL2 antibody (AB96233)

Immunocytochemistry/ Immunofluorescence analysis of A431 cells labeling LOXL2 with ab96233 at 1 : 500 (green). Cells were fixed in ice cold methanol for 5 minutes. Cells were co-stained with Hoechst 33342 (blue).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOXL2 antibody (AB96233)

Immunohistochemical analysis of Paraffin-embedded human esophageal carcinoma tissue labeling LOXL2 with ab96233 at 1/3000 dilution. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking : 3% H₂O₂, RT, 30min. Blocking : 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 min. Primary antibody incubation : 4°C overnight. Secondary antibody incubation : ABC HRP Kit (Rabbit IgG), 1 : 200, RT, 30min. Washing condition : PBS, 2 x 5 mins. Chromogen system : DAB.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOXL2 antibody (AB96233)

IHC image of ab96233 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96233, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LOXL2 antibody (AB96233)

Immunofluorescence analysis of 4% paraformaldehyde fixed at RT for 15 mins HeLa cells labelling LOXL2 protein at nucleus using ab96233 at a 1/500 dilution (Green). Alpha tubulin, a cytoskeleton marker stained by anti-alpha tubulin antibody at a 1/1000 dilution (Red).

• I-ELISA

Supplier Data

Indirect ELISA - Anti-LOXL2 antibody (AB96233)

Indirect ELISA analysis was performed by coating the plate with recombinant Human LOXL2 protein, His tagged (44.94-0.7 nM). Coated protein was probed with ab96233 (1 μg/mL). A Goat anti-rabbit IgG antibody (HRP) conjugated (1 : 10000) was used to detect the bound primary antibody.

• WB

Supplier Data

Western blot - Anti-LOXL2 antibody (AB96233)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-LOXL2 antibody (ab96233) at 1/5000 dilution

Lane 1:

Non-transfected (-) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Predicted band size: 87 kDa

false

• WB

Supplier Data

Western blot - Anti-LOXL2 antibody (AB96233)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-LOXL2 antibody (ab96233) at 1/500 dilution

Lane 1:

Wild-type (WT) HeLa cell extracts at 30 µg

Lane 2:

LOXL2 knockout (KO) HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 87 kDa

false

• WB

Supplier Data

Western blot - Anti-LOXL2 antibody (AB96233)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-LOXL2 antibody (ab96233) at 1/5000 dilution

Lane 1:

MCF-7 whole cell extracts at 30 µg

Lane 2:

MDA-MB-231 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 87 kDa

false

• WB

Supplier Data

Western blot - Anti-LOXL2 antibody (AB96233)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-LOXL2 antibody (ab96233) at 1/5000 dilution

All lanes:

U87-MG Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 87 kDa

false