Anti-LOXL2 antibody [EPR12733] - BSA and Azide free

• WB

Lab

Western blot - Anti-LOXL2 antibody [EPR12733] - BSA and Azide free (AB213598)

This data was developed using the same antibody clone in a different buffer formulation (ab179810).

Lanes 1-4 : Merged signal (red and green). Green - ab179810 observed at 105 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab179810 Anti-LOXL2 antibody [EPR12733] - C-terminal was shown to specifically react with LOXL2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264807 (knockout cell lysate ab257166) was used. Wild-type and LOXL2 knockout samples were subjected to SDS-PAGE. ab179810 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (<a href='/en-us/products/primary-antibodies/loxl2-antibody-epr12733-c-terminal-ab179810'>ab179810</a>) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

LOXL2 knockout HeLa cell lysate at 20 µg

Lane 3:

HeLa treated with 0.5nM CoCl2 for 6 hours whole cell lysate at 20 µg

Lane 4:

Untreated HeLa cell lysate at 20 µg

Predicted band size: 87 kDa

Observed band size: 105 kDa

false

• WB

Lab

Western blot - Anti-LOXL2 antibody [EPR12733] - BSA and Azide free (AB213598)

This data was developed using the same antibody clone in a different buffer formulation (ab179810).

Lanes 1-4 : Merged signal (red and green). Green - ab179810 observed at 105 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab179810 Anti-LOXL2 antibody [EPR12733] - C-terminal was shown to specifically react with LOXL2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261804 (knockout cell lysate ab257168) was used. Wild-type and LOXL2 knockout samples were subjected to SDS-PAGE. ab179810 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (<a href='/en-us/products/primary-antibodies/loxl2-antibody-epr12733-c-terminal-ab179810'>ab179810</a>) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

LOXL2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human LOXL2 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-loxl2-knockout-hela-cell-line-ab261804'>ab261804</a>)

Lane 3:

HeLa treated with 0.5nM CoCl2 for 6 hours whole cell lysate at 20 µg

Lane 4:

Untreated HeLa cell lysate at 20 µg

Predicted band size: 87 kDa

Observed band size: 105 kDa

false