Rabbit Recombinant Monoclonal LOXL4 antibody. Suitable for WB, IP, Flow Cyt (Intra) and reacts with Human, Mouse samples.
Images
![Western blot - Anti-LOXL4 antibody [EPR28300-90] (AB313797), expandable thumbnail](https://content.abcam.com/products/images/loxl4-antibody-epr28300-90-ab313797--western-blot-img419004.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LOXL4 antibody [EPR28300-90] (AB313797)")
![Western blot - Anti-LOXL4 antibody [EPR28300-90] (AB313797), expandable thumbnail](https://content.abcam.com/products/images/loxl4-antibody-epr28300-90-ab313797--western-blot-img419003.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LOXL4 antibody [EPR28300-90] (AB313797)")
![Flow Cytometry (Intracellular) - Anti-LOXL4 antibody [EPR28300-90] (AB313797), expandable thumbnail](https://content.abcam.com/products/images/loxl4-antibody-epr28300-90-ab313797--flow-cytometry-intracellular-img419071.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-LOXL4 antibody [EPR28300-90] (AB313797)")
![Immunoprecipitation - Anti-LOXL4 antibody [EPR28300-90] (AB313797), expandable thumbnail](https://content.abcam.com/products/images/loxl4-antibody-epr28300-90-ab313797--immunoprecipitation-img419012.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-LOXL4 antibody [EPR28300-90] (AB313797)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | Flow Cyt (Intra) | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Not recommended |
| Mouse | Tested | Expected | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Catalyzes the oxidative deamination of lysine and hydroxylysine residues in collagen and elastin, resulting in the formation of covalent cross-linkages, and the stabilization of collagen and elastin fibers.
Alternative names
LOXC, LOXL4, Lysyl oxidase homolog 4, Lysyl oxidase-like protein 4, Lysyl oxidase-related protein C
Recommended products
Rabbit Recombinant Monoclonal LOXL4 antibody. Suitable for WB, IP, Flow Cyt (Intra) and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR28300-90
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-LOXL4 antibody [EPR28300-90] (ab313797), expandable thumbnail](https://content.abcam.com/products/images/loxl4-antibody-epr28300-90-ab313797--western-blot-img419004.jpg "Western blot - Anti-LOXL4 antibody [EPR28300-90] (ab313797)")
Western blot - Anti-LOXL4 antibody [EPR28300-90] (ab313797)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: NIH/3T3(PMID:11292829).
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
All lanes: Western blot - Anti-LOXL4 antibody [EPR28300-90] (ab313797) at 1/1000 dilution
Lane 1: Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 30 µg
Lane 2: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 30 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 90 kDa
Exposure time: 125s
![Western blot - Anti-LOXL4 antibody [EPR28300-90] (ab313797), expandable thumbnail](https://content.abcam.com/products/images/loxl4-antibody-epr28300-90-ab313797--western-blot-img419003.jpg "Western blot - Anti-LOXL4 antibody [EPR28300-90] (ab313797)")
Western blot - Anti-LOXL4 antibody [EPR28300-90] (ab313797)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: LoVo, HeLa(PMID: 30728460).
The molecular weight observed is consistent with what has been described in the literature (PMID: 30728460).
This blot of Lane 4 was developed using a high sensitivity ECL substrate.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: Lane1-3: 15 seconds, Lane 4: 180 seconds.All lanes: Western blot - Anti-LOXL4 antibody [EPR28300-90] (ab313797) at 1/1000 dilution
Lane 1: A375 (human malignant melanoma epithelial cell) whole cell lysate at 20 µg
Lane 2: LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: Huh7 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 90 kDa
![Flow Cytometry (Intracellular) - Anti-LOXL4 antibody [EPR28300-90] (ab313797), expandable thumbnail](https://content.abcam.com/products/images/loxl4-antibody-epr28300-90-ab313797--flow-cytometry-intracellular-img419071.jpg "Flow Cytometry (Intracellular) - Anti-LOXL4 antibody [EPR28300-90] (ab313797)")
Flow Cytometry (Intracellular) - Anti-LOXL4 antibody [EPR28300-90] (ab313797)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell, Left) / A375 (human malignant melanoma epithelial cell, Right) cells labelling LOXL4 with ab313797 at 1/500 dilution (0.1 ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Low expression: HeLa (PMID: 30728460).
![Immunoprecipitation - Anti-LOXL4 antibody [EPR28300-90] (ab313797), expandable thumbnail](https://content.abcam.com/products/images/loxl4-antibody-epr28300-90-ab313797--immunoprecipitation-img419012.jpg "Immunoprecipitation - Anti-LOXL4 antibody [EPR28300-90] (ab313797)")
Immunoprecipitation - Anti-LOXL4 antibody [EPR28300-90] (ab313797)
LOXL4 was immunoprecipitated from 0.35 mg A375 (human malignant melanoma epithelial cell) whole cell lysate with ab313797 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313797 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: A375 (human malignant melanoma epithelial cell) whole cell lysate
Lane 2: A375 (human malignant melanoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab313797 in A375 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.All lanes: Immunoprecipitation - Anti-LOXL4 antibody [EPR28300-90] (ab313797) at 1/30 dilution
All lanes: A375 (human malignant melanoma epithelial cell) whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
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